:

19 7 31


..............................................................................................................1

1. ..................................................................................................1

2. ..................................................................................................1

3. ........................................................................................................1
3.1 ..................................................................................................................................... 1
3.2 ......................................................................................................................... 1

4. ...............................................................................................1

5. ...............................................................................................2
5.1 .............................................................................................................. 2
5.2 .......................................................................................................... 3
5.2.1 ................................................................................................................................. 3
5.2.2 ................................................................................................................................. 3
5.2.3 ................................................................................................................................. 4
5.3 ............................................................................................................................. 4
5.3.1 ......................................................................................................................... 4
5.3.2 ................................................................................................................. 5
5.3.3 ......................................................................................................................... 5
5.3.4 .......................................................................................................................... 5
5.3.5 ..................................................................................................................... 5
5.4 .............................................................................................................................. 5
5.4.1 ................................................................................................................................. 5
1/2 .................................................................................................. 5
................................................................................................ 6
......................................................................................................................... 6
d ........................................................................................ 6
e ................................................................................................. 6
f ................................................................................................................. 6
5.4.2 ............................................................................................................................. 7
a ................................................................................................................... 7
b ..................................................................................................................... 7
c ................................................................................................... 7

i
 5.4.3 ..................................................................................................................... 7
5.5 ......................................................................................................................... 7

6. ..................................................................................................8
6.1 ................................................................................................................. 8
6.2 ..................................................................................................................... 9
6.3 ......................................................................................................................... 9
6.3.1 ............................................................................................................. 9
6.3.2 ............................................................................................................. 9
6.4 ................................................................................................................. 9
6.4.1 .................................................................................................. 9
6.4.2 ................................................................................................................... 10
6.4.3 ........................................................................................................... 10
6.5 ........................................................................................................................... 10
6.5.1 ........................................................................................................... 10
6.5.2 ........................................................................................................... 11
6.5.3 ........................................................................................... 11
6.6 ................................................................................................................... 11
6.6.1 ........................................................................................................... 11
6.6.2 ............................................................................................................... 12
6.6.3 ............................................................................................................... 12

7. ................................................................................................ 13
7.1 ....................................................................................................... 13
7.1.1 ................................................................................................... 13
7.1.2 ................................................................................... 13
7.1.3 ........................................................................................... 13
7.1.4 ................................................................................................... 14
7.2 ........................................................................................................... 15
7.3 ............................................................................................................... 15
1 ................................................................................................................... 16

................................................................................... 17
a ........................................................................................................... 17
b ........................................................................................................... 17
c ........................................................................................... 17
d ..................................................................... 17

ii
 e ....................................................................................... 17

....................................................................................................... 18
a LP ..................................................................................................................... 18

....................................................................................................... 18
a ............................................................................................................... 18
b ................................................................................................ 18
c ................................................................................................................... 18
d ........................................................................................................... 19
e ........................................................................................................... 19
f ........................................................................................... 19

............................................................................................................ 20

. ............................................................................................ 20

. ............................................................................... 20
1. .............................................................................................................................. 20
1.1 ....................................................................................................................... 20
1.2 ....................................................................................................... 20
1.3 ........................................................................................................................... 20
a ........................................................................................................ 20
b ............................................................................................................... 21
c ........................................................................................................... 21
1.4 ............................................................................................................................... 21
1.5 ....................................................................................................................... 21
a ................................................................................... 21
b ........................................................................................................... 22
1.6 ....................................................................................................................... 22
1.7 ............................................................................................................................... 22
2. .............................................................................................................................. 23
2.1 ........................................................................................................................... 23
2.2 ................................................................................................ 23

. ...................................................................................... 23
1. ................................................................................................... 23
2. .......................................................................................................................... 24
3. ................................................................................................... 24

iii
. ....................................................... 25
1. .......................................................................................................... 25
2. .......................................................................................... 25

iv


EN 13697
JIS L 1902 JIS Z 2801

1.

2.

3.

3.1

3.2

4.

1

1
1
5
20 mm 2B

1.251086.25108 cfu/mL0.01 mL
1.5 w/v Cohn Fraction



53.58 mg/LCaCO3 Ca/Mg = 3
0.05 w/v 80
0.1 mL
3

5.
5.1

1) Stahylococcus aureus
2) Escherichia coli
2 2
WFCC : World Federation of Culture
CollectionJSCC : Japan Society of Culture Collection
2
2

ATCC6538P American Type Culture Collection

FDA209P Food and Drug Administration
Stahylococcus aureus
NBRC12732

ATCC8739 American Type Culture Collection
Escherichia coli NBRC3972

2
5.2

5.2.1

C2H5OH JIS K 8101 1

14


NaCl JIS K 8150
JIS K 8263





Cohn Fraction
JIS K 8122
JIS K 8159
NaOH JIS K 8576
HCl JIS K 8180
JIS K 9007
80Tween80

5.2.2

JIS G 0203 No.2 B 20

mm SUS304 1.01.5 mm

20 mm

JIS K 0950
18 mm150 mm

3

45 cm

35 mm
JIS P 3801

5.2.3

JIS K 3800
JIS B 9922
1525 1211 103 kPa

4548 1
371
1 60
pH 25 0.1 pH
160180

100 W
540660 nm
JIS K 0970 JIS R 3505 A

4 mm 10 L

5.3
5.3.1

160180 3060 1
1

4
5.3.2

121 103 Pa 1525
100

5.3.3

23

5.3.4

0.45 m

5.3.5

5.4

5.4.1

1/2

5.2.1 1 000 mL 1.5 g 2.5 g

pH 6.67.0 25

510 1 1/2

5.2.1 1 000 mL 3.0 g 5.0 g 15.0 g

pH 6.67.0 25

d
4548 510
1

b 610 mL
15
510
1

b5.2.2
1520 mL
1 510 1

5.2.1 1 000 mL 15.0 g 5.0 g 5.0

g 15.0 g
pH 7.17.5 25
4548
510 1

5.2.1 1 000 mL 2.5 g 5.0 g 1.0 g 15.0 g

pH 7.0
7.2 25
4548 5

6
10 1

5.4.2

34.0 5.2.1 500 mL

H 6.87.2 25
1 000 mL
510 6

1.25 mL 5.2.1 1 000 mL 100 mL 9.0

mL 121 1525
510 1

5.2.1 1 000 mL 8.5 g

510
1

a b0.85 800

510 1

5.4.3

59.03 g 27.21 g
5.2.1 500 mL 1 000 mL

CaCO3 53.58 g/L

1

5.5
2

7

10 L 371
24
5.4.1 c

1 2
371 2448
510 1
10
1
2 1





10

6.
6.1
5.5 5.4.1 d371 18
24

8
37 1824

6.2
2n nn32n n n
5.2.2 .a

6.3
6.3.1

53.58 mg/LCaCO3
1 53.58 mg/L
CaCO3 5.4.3 1 000

1
1

6.3.2

0.05 w/v 80 5.2.1

1

6.4
6.4.1

5.2.1 5.2.1 Cohn Fraction.1 30 g/L

9
6.4.2

1
5.4.1 a 1/2 5 mL 35 g
6.1 1
150 3

3
1 mL 1/2
2.5108 12.5108
cfu/mL4 1
1 2

3 0.5 mL
5.2.1 5.4.2 10
30 660 nm


4
cfu colony forming unit

6.4.3

1 6.4.2 1.0 mL 6.4.1 1.0 mL

2
6.4.1 6.4.2

6.4.2

6.5
6.5.1

6.2 1
2
6.4.3 0.01 mL
1 mm

10
5

5

60 60 60




3

6.5.2

6.5.1
0.1 mL

6.5.3

5.2.2 10.0 mL
5 6.5.2
5

150
1

6.6
6.6.1

6.5.3 1.0 mL 5.4.2 9.0 mL

11
 1.0 mL
9.0 mL 10

6.6.2

6.6.1 1.0 mL 2
1 4548 5.4.1 b 5.4.1 e
5.4.1 f 1520 mL
371 4048

30300
1.0 mL 30
300 300TNTC6
1

6 Too Numerous To Count

2000 1.2 3 1
2004, 2 1

6.6.3

N = C 10M 10 = C 10M+1 1
N cfu/
C 2
M
0
10 mL
3 2 1
10
n n 3
2 1010

12
 1

7.
7.1
7.1.1

6.4.2 1 N0 6.6.1 6.6.2

N0 = C 10M 2
N0 cfu/mL
C 2
M
N0 3 2

7.1.2

N07.1.1 Nt
Nt = N0 0.5 0.01 = N0 / 2003
Nt cfu/
0.5
0.01 mL
Nt 3 2

7.1.3

NC 6.6.3 NC n
n AvC 7.1.2 Nt Lt

-1.0 Avc - Lt +0.54

Avc n

Lt Nt
Lt Avc 3 2

13
7.1.4

5 1

1
7.1.1 2.510812.5108 cfu/mL
2
n CVc
5
CVC SDC / AvC 100 10 5
CVC 7
SDC n

3
n i
7.2 6
i CV7
300 cfu/ Av

i AvC LSi 6
LSi n

i
CV SD / Av 100 7
= 20
= 25
CV i7
SD i n
Av i n
7
n 5 i3
7 2 1

4

5

14
 7.1.3 7.1.3
7
) CVC CV 1

7.2
2
1
= AvC - AvS8
AvS n
3 2

7.3

a
1
2
3
b
1
2
3
4
5
c
1
2
3
4
5
6
7
8
d
1
2
3

CVC
CV


4

15
 1
JIS B 9922
JIS G 0203
JIS K 0950
JIS K 0970
JIS K 3800
JIS K 8101 99.5
JIS K 8122
JIS K 8150
JIS K 8159
JIS K 8180
JIS K 8263
JIS K 8576
JIS K 9007
JIS P 3801
JIS R 3505
JIS Z 2801

16


6.3.1

6.4 6.4.2
6.6.1 6.6.2 7.1.1

10.0 mL a 0.1 mL
35 b 0.01 mL
35 5
5 35 10
6.6.1 6.6.2
5.4.2 0.1 mL

c2 10.0 mL
b 0.01 mL 35
5 5 35
10 6.6.1 6.6.2

1 b 2.510812.5108 cfu/mL
2 c
5.4.2 50
3 d

17
 50

a LP

5.2.1 1 000 mL 1.0 g 0.7 g

80 20.0 g H 7.07.4 25

510
1 LP

6.2
< 6.2 >
ExtranMA01MERCK 5
10

<
6.4.1 >

1/2
150
1

18
 2
< 6.4.2 >
d

< 6.5.1 >

2n

30 < 6.5.2 >

2n
2n 5

< 6.5.3 >

19


.
EN 13697 Chemical
disinfectants and antiseptics - Quantitative non-porous surface test for the evaluation of
bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic
and institutional areas - Test method and requirements without mechanical action phase 2/step
2JIS Z 2801
JIS L 1902

.
1.
1.1

1 EN 13697

1.2

EN 13697 5

5 < 6.5.2 >

1.3

< 5.1 >

20
b

1 log10
5 2.510812.5108 cfu/mL
0.01 mL
1.251066.25106 cfu/
1 log10 4
< 6.4.2 7.1.4 >

EN 13697 20 mm 0.05 mL

EN
13697 1/5 0.01 mL
EN 13697 5
< 6.5.1 >

1.4

EN 13697 Cohn Fraction

2 0.3 g/L 3 g/L

3 g/L
30 g/L

< 6.4.1 >

1.5

2.34.4 DH 1
14.2 DH 3 DH 4 DH

21
 JIS K 3362
9.2 b 14
3 DH < 6.3.1
>
1 DH 100 mL
mg DHCaCO3 mg/L 0.056

EN 13697
0.1 mL

() 0.1 mL

1 0.81.0 mL
1020 cm
57 cm 2040 cm2
3.1 cm2 0.1 mL
< 6.5.2 >

1.6

80
0.05 w/v 80
0.05 w/v 80
< 6.3.2 >

1.7

JIS L 1902 1/20

1.3 c 1/5
1/2
< 5.4.1 a>

22
2.

2.1

JIS Z 2801 2
JIS Z 2801 1824 < 6. 1 >

2.2

EN 13697 2 1
2

2
1
EN 13697 37

EN 13697 1/5 0.01 mL

5
< 6.5.1 >

.
1.
7.1.4 2 3

WG
300 cfu/i
1 300 cfu/

23
 300 cfu/
30 30
2
i 1
Avi

n 5
(i) 1 3

2 20002000p.51-53
1990p.70-77

2.

3
3
I e 2

1
2

3.
7.1.4 5

1 1
0.5
1 1-1
< 7.1.3 >

24
.

5.2.1 5.2.2

1.
Cohn Fraction

ALBUMIN,BOVINE Fraction Powder A-2153 SIGMA

2.
5.2.2

5350013 2-2-31
TEL06-6953-1661 FAX06-6955-1850

25


1030027 31311
0332714301
0332811870

26