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Calculations in g/mL

and ng/mL
Mr. Sagar Kishor Savale, M. Pharm (P. ceutics), R. C. Patel,
Institute of Pharmaceutical Education and Research,
Shirpur, 425405, MS, India. Mob. No. 9960885333,
8830658405, Email: savalesagar484@gmail.com
Calculations in g/mL and ng/mL | SAGAR SAVALE

Calculation in g/mL
Basic
10 mg drug added in 100 ml solvent (100 g/mL)
10 mg in 10 mL (1000 g/mL)
1 mg in 100 mL (10 g/mL)
100 mg in 100 mL (1000 g/mL)

Calibration curve: (g/mL)


10 mg drug was added in 100 ml methanol or any solvent (100 g/mL) (Stock solution)

(Take required quantity in above prepared stock solution for further dilutions)

0.1 mL diluted with 10 ml of methanol 1 g/mL


0.2 mL diluted with 10 ml of methanol 2 g/mL
0.3 mL diluted with 10 ml of methanol 3 g/mL
0.4 mL diluted with 10 ml of methanol 4 g/mL
0.5 mL diluted with 10 ml of methanol 5 g/mL
0.6 mL diluted with 10 ml of methanol 6 g/mL
0.7 mL diluted with 10 ml of methanol 7 g/mL
0.8 mL diluted with 10 ml of methanol 8 g/mL
0.9 mL diluted with 10 ml of methanol 9 g/mL
1.0 mL diluted with 10 ml of methanol 10 g/mL
1.1 mL diluted with 10 ml of methanol 11 g/mL
1.2 mL diluted with 10 ml of methanol 12 g/mL
1.3 mL diluted with 10 ml of methanol 13 g/mL
1.4 mL diluted with 10 ml of methanol 14 g/mL
1.5 mL diluted with 10 ml of methanol 15 g/mL
1.6 mL diluted with 10 ml of methanol 16 g/mL
1.7 mL diluted with 10 ml of methanol 17 g/mL
1.8 mL diluted with 10 ml of methanol 18 g/mL
1.9 mL diluted with 10 ml of methanol 19 g/mL

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Calculations in g/mL and ng/mL | SAGAR SAVALE

2.0 mL diluted with 10 ml of methanol 20 g/mL


2.1 mL diluted with 10 ml of methanol 21 g/mL
2.2 mL diluted with 10 ml of methanol 22 g/mL
2.3 mL diluted with 10 ml of methanol 23 g/mL
2.4 mL diluted with 10 ml of methanol 24 g/mL
2.5 mL diluted with 10 ml of methanol 25 g/mL
2.6 mL diluted with 10 ml of methanol 26 g/mL
2.7 mL diluted with 10 ml of methanol 27 g/mL
2.8 mL diluted with 10 ml of methanol 28 g/mL
2.9 mL diluted with 10 ml of methanol 29 g/mL
3.0 mL diluted with 10 ml of methanol 30 g/mL
3.1 mL diluted with 10 ml of methanol 31 g/mL
3.2 mL diluted with 10 ml of methanol 32 g/mL
3.3 mL diluted with 10 ml of methanol 33 g/mL
3.4 mL diluted with 10 ml of methanol 34 g/mL
3.5 mL diluted with 10 ml of methanol 35 g/mL
3.6 mL diluted with 10 ml of methanol 36 g/mL
3.7 mL diluted with 10 ml of methanol 37 g/mL
3.8 mL diluted with 10 ml of methanol 38 g/mL
3.9 mL diluted with 10 ml of methanol 39 g/mL
4.0 mL diluted with 10 ml of methanol 40 g/mL
4.1 mL diluted with 10 ml of methanol 41 g/mL
4.2 mL diluted with 10 ml of methanol 42 g/mL
4.3 mL diluted with 10 ml of methanol 43 g/mL
4.4 mL diluted with 10 ml of methanol 44 g/mL
4.5 mL diluted with 10 ml of methanol 45 g/mL
4.6 mL diluted with 10 ml of methanol 46 g/mL
4.7 mL diluted with 10 ml of methanol 47 g/mL
4.8 mL diluted with 10 ml of methanol 48 g/mL
4.9 mL diluted with 10 ml of methanol 49 g/mL
5.0 mL diluted with 10 ml of methanol 50 g/mL
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Calculations in g/mL and ng/mL | SAGAR SAVALE

5.1 mL diluted with 10 ml of methanol 51 g/mL


5.2 mL diluted with 10 ml of methanol 52 g/mL
5.3 mL diluted with 10 ml of methanol 53 g/mL
5.4 mL diluted with 10 ml of methanol 54 g/mL
5.5 mL diluted with 10 ml of methanol 55 g/mL
5.6 mL diluted with 10 ml of methanol 56 g/mL
5.7 mL diluted with 10 ml of methanol 57 g/mL
5.8 mL diluted with 10 ml of methanol 58 g/mL
5.9 mL diluted with 10 ml of methanol 59 g/mL
6.0 mL diluted with 10 ml of methanol 60 g/mL
6.1 mL diluted with 10 ml of methanol 61 g/mL
6.2 mL diluted with 10 ml of methanol 62 g/mL
6.3 mL diluted with 10 ml of methanol 63 g/mL
6.4 mL diluted with 10 ml of methanol 64 g/mL
6.5 mL diluted with 10 ml of methanol 65 g/mL
6.6 mL diluted with 10 ml of methanol 66 g/mL
6.7 mL diluted with 10 ml of methanol 67 g/mL
6.8 mL diluted with 10 ml of methanol 68 g/mL
6.9 mL diluted with 10 ml of methanol 69 g/mL
7.0 mL diluted with 10 ml of methanol 70 g/mL
7.1 mL diluted with 10 ml of methanol 71 g/mL
7.2 mL diluted with 10 ml of methanol 72 g/mL
7.3 mL diluted with 10 ml of methanol 73 g/mL
7.4 mL diluted with 10 ml of methanol 74 g/mL
7.5 mL diluted with 10 ml of methanol 75 g/mL
7.6 mL diluted with 10 ml of methanol 76 g/mL
7.7 mL diluted with 10 ml of methanol 77 g/mL
7.8 mL diluted with 10 ml of methanol 78 g/mL
7.9 mL diluted with 10 ml of methanol 79 g/mL
8.0 mL diluted with 10 ml of methanol 80 g/mL
8.1 mL diluted with 10 ml of methanol 81 g/mL
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Calculations in g/mL and ng/mL | SAGAR SAVALE

8.2 mL diluted with 10 ml of methanol 82 g/mL


8.3 mL diluted with 10 ml of methanol 83 g/mL
8.4 mL diluted with 10 ml of methanol 84 g/mL
8.5 mL diluted with 10 ml of methanol 85 g/mL
8.6 mL diluted with 10 ml of methanol 86 g/mL
8.7 mL diluted with 10 ml of methanol 87 g/mL
8.8 mL diluted with 10 ml of methanol 88 g/mL
8.9 mL diluted with 10 ml of methanol 89 g/mL
9.0 mL diluted with 10 ml of methanol 90 g/mL
9.1 mL diluted with 10 ml of methanol 91 g/mL
9.2 mL diluted with 10 ml of methanol 92 g/mL
9.3 mL diluted with 10 ml of methanol 93 g/mL
9.4 mL diluted with 10 ml of methanol 94 g/mL
9.5 mL diluted with 10 ml of methanol 95 g/mL
9.6 mL diluted with 10 ml of methanol 96 g/mL
9.7 mL diluted with 10 ml of methanol 97 g/mL
9.8 mL diluted with 10 ml of methanol 98 g/mL
9.9 mL diluted with 10 ml of methanol 99 g/mL
10.0 mL diluted with 10 ml of methanol 100 g/mL
*(I used methanol as a solvent but, you can used your solvent and dilutions was same in all solvents)

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Calculations in g/mL and ng/mL | SAGAR SAVALE

Calibration curve: (ng/mL)


How to prepared concentration in ng/mL
Concentration in ng/mL was prepared in two or more than two ways but, here we
discussed only two ways,
One Method:
1 mg of drug in 100 mL of methanol or any other solvent (10 g/mL)

(Take required quantity of above solution for further dilution to prepared


concentration ng/mL)

0.1 mL Diluted with 10 mL Methanol 100 ng/mL


0.2 mL Diluted with 10 mL Methanol 200 ng/mL
0.3 mL Diluted with 10 mL Methanol 300 ng/mL
0.4 mL Diluted with 10 mL Methanol 400 ng/mL
0.5 mL Diluted with 10 mL Methanol 500 ng/mL
0.6 mL Diluted with 10 mL Methanol 600 ng/mL
0.7 mL Diluted with 10 mL Methanol 700 ng/mL
0.8 mL Diluted with 10 mL Methanol 800 ng/mL
0.9 mL Diluted with 10 mL Methanol 900 ng/mL
1.0 mL Diluted with 10 mL Methanol 1000 ng/mL
(100 to 1000 ng/mL solutions was prepared)

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Calculations in g/mL and ng/mL | SAGAR SAVALE

Second Method:
10 mg drug in 10 mL of Methanol or any other solvent (1000 g/mL)

(Take required quantity of above solution for further dilution to prepared


concentration g/mL)

1 mL Diluted with 100 mL of Methanol 10 g/mL


2 mL Diluted with 100 mL of Methanol 20 g/mL
3 mL Diluted with 100 mL of Methanol 30 g/mL
4 mL Diluted with 100 mL of Methanol 40 g/mL

(Take required quantity of above solution for further dilution to prepared


concentration ng/mL)

0.1 mL Diluted with 10 mL of Methanol 100 ng/mL


0.2 mL Diluted with 10 mL of Methanol 200 ng/mL
0.3 mL Diluted with 10 mL of Methanol 300 ng/mL
0.4 mL Diluted with 10 mL of Methanol 400 ng/mL
(100 to 400 ng/mL solutions was prepared)

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Calculations in g/mL and ng/mL | SAGAR SAVALE

Other Calculations Variables


Units of Mass
1 kg = 1000 gm.
1 gm = 1000 mg.
1mg = 1000 g.
1 g = 1000 ng. (Nano gram)
1 ng = 1000 pg. (Pico gram)
1 pg = 1/1000 ng.

Units of Volume
1 L = 1000 mL.
1 mL = 1000 l.
1 l = 1/1000 mL.

Units of Length
1 cm = 1000 mm.
1 mm = 1000 m.
1 m = 1000 nm.
1 nm = 1/1000 mm.

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