100

:101421

6445
E-mail 084439@mail.fju.edu.tw


IACUC:

()()



(IACUC)





Institutional Animal Care and Use
CommitteeIACUC







(pilot study)

IACUC



Reduction
Replacement
Refinement
 3Rs

1959 Russell & Burch The Principles of Humane

Experimental Technique
Replacement


Reduction

Refinement

The importance and value of the 3Rs- not just in
terms of animal welfare but as an adjunct to good
science.
 Animal Use Protocol Review
Replacement/ Reduction: Refinement:

http://140.136.128.217/exp/index_login.php

http://140.136.128.217/exp/index_login.php

(LDAP)

-1

-2
Key elements of animal use protocol

Key elements of animal use protocol

/a / b

a
b

Animal model selection

Reduction of animal use/numbers
Wildlife field Studies
 Key elements of animal use protocol

1.
2.

1.


2.
Key elements of animal use protocol

Necessity of the research

Replacement/Alternatives to animal use
Reduction of animal use/numbers
 Key elements of animal use protocol

()

Physical restraint

Anesthesia , Survival surgery

Setting endpoints , refinement


PentobarbitalKetamine/Xylazine :

Ether :

Urethane :

Avertin (Tribromoethanol) :

Chloral hydrate :

Zoletil (Zolazepam and Tiletamine) :

Key elements of animal use protocol

Study Endpoint
z
z
z

Early Endpoint (Humane endpoint)

z
z


.
125 g 125 g~ 1 kg 1 kg~ 5 kg
/ /


.
.

Barbiturate,
3

Barbiturate,
3

()

KCl
(1-2 meq/kg)

Ether()
IACUC

Barbiturate
KetamineBarbiturate

CO2

200

14
CO2


14

CO2

20-30 % /
10-20

CCAC guidelines on animal euthanasia,2010

Key elements of animal use protocol

1
2
3










http://www.rdo.fju.edu.tw/comm3.php


3Rs5Rs

Reduction
Replacement
Refinement
Responsibility
Respect
 ResponsibilityIACUC

Respect for all life

Its not only materials, its a LIFE.


//



xxxA BALB/c 1 200 180

xxxA SD 1 50 38

//

xxxA BALB/c 180 -CO2 /

xxxA SD 38 /

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[
(
(B)
)
(A2)
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(A1)

30(50+0-
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10 4-2-1

50 20

30
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)
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20 4-2-1

70 30

1. (IACUC)

2.()

3.
a.


b.



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