ABSTRACT

A variety of environment problems now affect the entire world. One of these largest

problems is oil spill. As a matter of fact, an oil spill is a form of pollution because it

releases liquid petroleum hydrocarbon into the environment due to human activity. The

disposal of these substances in natural environment is hazardous for the natural

system. The only approach of cleaning up these hydrocarbons (oil and grease) is

bioremediation.

Bioremediation can be defined as any process that makes use of microorganisms,

green plants or their enzymes to return the natural environment altered by contaminants

to its original condition without giving adverse effects to the environment.

This study utilized three treatments with three replicates explicitly the control or

untreated sample (To), crude enzyme from fermented peanuts (T 1) and B. subtilis (T2).

Samples from each treatment were subjected to different laboratory analyses such as

oil and Grease, Total Solids and pH determination with 96-hr interval and the data was

analyzed by Pearson Product-Moment Correlation Method.

Results revealed that among the three treatment 1(crude enzyme from fermented

peanuts) has the highest degradation of the oil and grease concentration and there was

a strong correlation between the period of remediation and physicochemical parameters

such as Total Solids, Oil and Grease and pH.

Results concluded that the crude enzyme from fermented peanuts is efficient in

degrading oil and grease.