Connective and supporting

tissues
Krisztinn Pva Beta
Skin (thick) (HE) 1x
epidermis
keratinized stratified
squamous epithelium

dermis

hypodermis
 skin (HE) 20x

epithelium basal membrane

Between the epithelium and CT
electromicroscopic structure

dermis - stratum papillare/

papillary layer
loose connective tissue

dermis
capillary - stratum reticulare/
endothel reticular layer
(red blood cells
dermal papilla
 skin (HE) 20x
dermis str. reticulare
dense irregular connective tissue

nucleus of fibrocyte
collagen fiber
(eosinophil)

artefact
(during the stainig method
the tissues shrink )
 skin (HE) - 4x
hypodermis
loose connective tissue
adipose vessels
tissue

peripherial nerve

sensory nerve ending

(Vater-Pacini corpuscule)
 Skin (HE) 20x
hypodermis
unilocular (white) adipose tissue

capillary
adipocyte
special simple
sorrounded:
squamous epithelium =
basal membrane (EM)
endothel
reticular fibers (not visible)
red blood cells

nucleus of adipocyte

lipid droplet
(the lipid dissolved
collagen fiber
during the embedding
and staining method)
Skin (thick)
staining: hematoxylin eosin (HE)
connective tissue types located in the section:
fiber rich CT
loose CT
dense irregular CT
white adipose tissue
connective tissue fibers located in the section:
collagen fibers eosinophil
reticular fibers eosinophil, extremely fine => we can not identify
(demonstration occurs whit silver argyrophil)
connective tissue cells located in the section:
Fix cells
fibrocyte
adipocyte
melanocyte we can nat distinguish
Mobile cells: macrophages, mast cells, lymphocytes, granulocytes,
plasma cells identified with difficulty
 Elastic cartilage 1x
staining:
orcein elastic fibers
hematoxylin - nucleus

perichondrium

elastic cartilage

fiber rich connective

tissue
 Elastic cartilage 20x

elastic fibers
chondron (produced by chondroblasts)
chondrocyte
lacuna

perichondrium
stratum chondroblasticum
stratum fibrosum
dense irregular CT
fibrocyte

chondroblast
Elastic cartilage 20x orcein

Muscular type artery

internal elastic membrane (IEM)
elastic membrane
(produced by smooth
muscle cells)

Is NOT always present

Elastic cartilage

staining:
hematoxylin basic dye, binds to negatively charged molecules, e.g.
DNA (in the nucleus)
orcein: indicates the elastic fibers, membranes
connective tissue types located in the section:
elastic cartilage
dense irregular CT
connective tissue fibers located in the section:
elastic fibers
collagen fiber (orcein does not stain)
connective tissue cells located in the section:
Fix cells
chondroblast
chondrocyte
fibrocyte
 Compact bone, ground 1x
lamellar (matured/ secondary) bone tissue
staining: unstained
The inorganic substance is ovservable

laminae generales externae/

outer circumferential lamellae

compact substabce

laminae generales internae/

inner circumferential lamellae
 Compact bone, ground - 10x

osteon/
Haversian system
Volkmanns
canal

interstitial lamellae
(remnant of earlier
osteon)

Haversian canal
Compact bone, ground - 40x

laminae speciales/
concentrical lamellae

lacuna

canaliculi

Haversian canal
lamellar (matured/ secondary) bone tissue

staining: unstained
Ground the inorganic substance is observable
connective tissue types located in the section:
lamellar (matured/ secondary) bone tissue
connective tissue fibers located in the section:
There are NOT fibers in the section
remark: the lamellae contain spiral collagen fibers (as organic
element)
connective tissue cells located in the section:
There are NOT cells in the section
remark: in the living bone the osteocytes are sitting in the
lacunae, and their processes are located in the canaliculi
 chondral ossification (HE) - 1x
long bone epiphysis
(hyaline cartilage)
epiphysis
epiphyseal articular
(hyaline cartilage)
plate cartilage
diaphysis

striated muscle
tissue

secundary center of
primary center of ossification, filled with
ossification, filled with red bone marrow
red bone marrow primary (woven)
bone
 Enchondral ossification - 10x

resting zone

proliferative zone
hypertrophic zone
calcification zone

ossification zone
Resting zone: hyaline cartilage
proliferative zone: chondrocytes divide and arrange
themselves in parallel columns
hypertrophic zone: distended chondrocytes, with
accumulated glycogen in their cytoplasm
calcification zone: death of chondrocytes, the ECM
becomes calcified
ossification zone:bone tissue appears
 Enchondralis ossification - 40x
resting zone hyaline cartilage

chondrocyte
lacuna
chondron

lacuna
chondron pericellular perichondrium
matrix (dense irregular CT)
(contains vessels)
territorial matrix
(capsule)
interterritorial matrix
(between the
chondrocyte chondrons)
 Enchondral ossification - 20x
hyaline cartilage
hypertrophic zone proliferative resting zone
zone
chondrocytes divide and
arrange themselves in
parallel columns
swollen cells, accumulated
glycogen in their cytoplasm
chondron

interterritorial
matrix
artefact

ECM:
Fine type II collagen
proteoglicans: e.g. aggrecan
(GAG chains contain
chondroitin sulfate, keratan
sulfate)
Hyaluronic acid basophil
 Enchondral ossification - 40x
ossification zone calcification zone

capillary degenerated
chondrocytes
chondroclast
Red bone marrow
stroma: reticular CT
(network of reticular fibers)

cartilage septum
the ECM becomes calcified
- basophil
 Enchondral ossification - 40x

primary bone matrix - osteoprogenitor cells

osteoid
(eosinophil)
chondroclast or
osteoclast
cartilage
ground substance
chondroclast
(basophil)
osteocyte

osteoblast

megakariocyte
Enchondral ossification - 40x

osteoprogenitor cells osteoblast

osteoblast

osteoid
osteocyte (collagen fibers
cartilage ground - eosinophil)
substance
(basophil)
 Enchondral ossification - 20x
around the long bone
internal elastic membrane (IEM)
elastic membrane
artery (HE doesnt stain)
Is NOT always present in your
section

vein

striated muscle tissue

bone tissue

periosteum dense
irregular CT
Enchondral ossification

staining: hematoxylin eosin (HE)

connective and supporting tissue types located in the
section:
Hyaline cartilage
woven (primer) bone tissue
reticular CT
Dense irregular CT
connective tissue fibers located in the section:
collagen fibers
Reticular fibers we can not identify with HE staining
connective tissue cells located in the section:
fibrocyte
chondroblast, chondrocyte, chondroclast
osteoblast, osteocyte, osteoclast, osteoprogenitor cell
developping and matured leukocytes