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INTRODUCTION
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Hexavalent chromium has been detected in groundwater across the
United States due to industrial and military operations, including plating,
painting, cooling-tower water and chromate production. Because inhalation
of Chromium (VI) can cause lung cancer in some persons exposed to a
sufficient airborne concentration, questions have been raised about the
possible hazards associated with exposure to Chromium (VI) in tap water via
ingestion, inhalation, and dermal contact. Although ingested Chromium (VI)
is generally known to be converted to Chromium (III) in the stomach
following ingestion, prior to the mid-1980s a quantitative analysis of the
reduction capacity of the human stomach had not been conducted.
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OBJECTIVES
THEORY
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plastic or metal products such as shower heads or car bumpers. Although not
commonly seen by the general public, one of the most important uses of
chrome plating is to apply a hard, smooth surface to machine parts such as
crankshafts and printing rollers. This process is known as "hard" chrome
plating. Chromic acid anodizing is another industrial metal finishing process
which uses hexavalent chromium.
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absorbed by the chemical species in the sample is equivalent to the
difference in the amount of light before it enters the sample and after it exits
the sample. For the purpose of light to be absorbed by chemical species, the
light must be set to a specific wavelength. Every chemical species absorbs
distinct wavelengths of light. In absorption spectroscopy, the wavelengths of
light absorbed by the in solution are detected.
M1V1 = M2V2
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Thus, if we plot a graph of absorbance versus concentration of the compound
solution, we get a straight line passing through the origin .A compound with
high molar absorbance is very effective at absorbing light. Hence, low
concentrations of a compound and high molar absorbance can be easily
detected by the spectroscopy. The Beer-Lamberts Law
A = LC
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APPARATUS
1. Spectrometer.
2. 10 mL of cuvette.
3. Pipette.
4. Dropper.
5. Measuring cylinder.
6. 50 mL volumetric flask.
7. Stopper.
8. Beaker
MATERIALS
1. Distilled water.
2. Stock solution of chromium (VI) solution (200ppm)
3. 7 set of diluted chromium (VI) solutions which are 200 ppm, 100 ppm, 80
ppm, 60 ppm, 40 ppm, 20 ppm and 5 ppm.
4. Lake Sample
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PROCEDURE
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RESULT
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Lake Water Sample Absorbance Value
0.06
f(x) = 0x
0.05
0.04
0.03
Absorbance Value
0.02
0.01
0
10 30 50 70 90 110 130 150 170 190 210
0 20 40 60 80 100 120 140 160 180 200
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Figure 1 : Graph of absorbance value versus concentration of Chromium (VI)
solution (p.p.m)
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CALCULATIONS
For 15 p.p.m
1L
20 mL Chromium solution = 0.02 L Chromium solution
1000 mL
n= MV
g . mol
0.005 0.02 L = 0.0001 g.mol Chromium
L
52 g chromium
0.0001 g.mol Chromium = 0.0052 g of chromium
1 mol chromium
For 20 p.p.m
1L
20 mL Chromium solution = 0.02 L Chromium solution
1000 mL
n= MV
g . mol
0.02 0.02 L = 0.0004 g.mol Chromium
L
52 g chromium
0.0004 g.mol Chromium = 0.0208 g of chromium
1 mol chromium
For 40 p.p.m
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mg. mol 1g g . mol
40 ppm = 40 L 1000 mg. mol = 0.04 L
1L
20 mL Chromium solution = 0.02 L Chromium solution
1000 mL
n= MV
g . mol
0.04 0.02 L = 0.0008 g.mol Chromium
L
52 g chromium
0.0008 g.mol Chromium = 0.0416 g of chromium
1 mol chromium
For 60 p.p.m
1L
20 mL Chromium solution = 0.02 L Chromium solution
1000 mL
n= MV
g . mol
0.06 0.02 L = 0.0012 g.mol Chromium
L
52 g chromium
0.0012 g.mol Chromium = 0.0624 g of chromium
1 mol chromium
For 80 p.p.m
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1L
20 mL Chromium solution = 0.02 L Chromium solution
1000 mL
n= MV
g . mol
0.08 0.02 L = 0.0016 g.mol Chromium
L
52 g chromium
0.0016 g.mol Chromium = 0.0832 g of chromium
1 mol chromium
1L
20 mL Chromium solution = 0.02 L Chromium solution
1000 mL
n= MV
g . mol
0.1 0.02 L = 0.002 g.mol Chromium
L
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Mass = no of moles molar mass
52 g chromium
0.002 g.mol Chromium = 0.104 g of chromium
1 mol chromium
1L
20 mL Chromium solution = 0.02 L Chromium solution
1000 mL
n= MV
g . mol
0.2 0.02 L = 0.004 g.mol Chromium
L
52 g chromium
0.004 g.mol Chromium = 0.208 g of chromium
1 mol chromium
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DISCUSSION
The aims of this experiment was to to determine the Chromium (VI) content
which present in simulated lake water sample using a spectrophotometer, to
demonstrate the proper method of diluting solution to prepare a series of
standard solution in the range of 5-200 part per million (p.p.m) to be used in
the calibration of the spectrophotometer, and thus, to analyze whether the
simulated lake water sample test is suitable for drinking water and
agriculture purposes .
M1V1 = M2V2
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CONCLUSION
The proper method of diluting solution to prepare a series of standard solution in the
range of 5 200 part per million (Ppm) is demonstrated in this experiment and it is used
in the calibration of the spectrophotometer. The Chromium (VI) content which present in
simulated lake water sample is . It is obtained using a spectrophotometer. Drinking
water should be less than 500 ppm, and water for agriculture should be less than 1200
ppm. The lake water sample is 98 p.p.m.
RECOMMENDATIONS
These are the recommendations and precautions that can be considered in the
experiment to get a better and accurate result. Firstly, all the apparatus that are used
like cuvette, beaker, dropper and equipment like spectrophotometer are in good
condition. Secondly, make sure the volume of the standard solution used must be taken
correctly by avoiding parallax error. Thirdly, obtain the lake water sample from more
places. In this experiment, all groups only obtain the sample from two lakes. Fourthly,
the standard solution that is used should be a hundred percent pure and stable at room
temperature. Lastly, the spectrophotometer. This spectrophotometer is a precision
instrument and deserves careful handling. Always wipe fingerprints and spilt sample off
the outside of the cuvette before measuring. Dont forget to close the door to the cuvette
compartment before reading the result.
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REFERENCE / APPENDIX
https://www.scribd.com/doc/39166236/Determination-of-Chromium-VI-
Concentration-via-Absorption-Spectroscopy-Experiment
https://www.scribd.com/doc/110293635/determination-of-concentration-
of-chromium
http://chemistry.about.com/od/analyticalchemistry/a/spectroscopy.htm
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