Professional Documents
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Basics of Microbiology:
Introduction to Structure and
Function of Bacteria
Dr Charlene Kahler
Rm 2.03A
L Block QE II Medical Center
email: charlene.kahler@uwa.edu.au
What can go wrong?
Toddler Tarliah O'Connell's tooth agony 'ignored' for days
Gemma Jones
A TODDLER suffering a mouth infection so severe it later required surgery and teeth removal
was given only Panadol while the infection worsened at a hospital.
Tarliah O'Connell, from Penrith, NSW, was moaning in pain after four days at Nepean Hospital and the painful
infection then spread across her face.
After being given only Panadol and Nurofen, a desperately sick Tarliah was transferred to The Children's
Hospital, Westmead, last Thursday.
Her mother Raquel said Tarliah was immediately given morphine to control her terrible pain. She said her
daughter has been left so traumatised she was now scared of medical staff.
Tarliah has undergone serious facial surgery to treat the infection which even spread to her throat.
Objectives for this lecture:
At the end of this lecture, you should be able to:
Explain the history of microbiology in dental hygiene
Describe the difference between eukaryotes and bacteria
Explain the diversity of microorganisms in relation to size, shape
and arrangement.
Draw a figure showing the basic features of a bacterial cell
Describe the function of the following components of a bacterial
cell: nucleiod, cytoplasm, cytoplasmic membrane, cell wall.
Explain binary fission using a diagram
Describe the principle behind the Gram stain which
differentiates bacteria into Gram positive and Gram negative
categories.
List the structural differences and similarities of Gram negative
and positive bacterial cell walls and explain how these features
impart different properties to the cell.
What are: capsules, pili, flagella and endospores?
Microbiology
Definition: The study of microorganisms
includes bacteria, archaea, yeasts, fungi
(molds), viruses, algae, and protozoans
involves understanding
How do they spread from location to another?
Why do some cause disease and others are
beneficial?
What is their role in the environment?
How do they operate?
Microbiology and dental hygiene
1676: Anton van Leeuwenhoek
first person to observe
microorganisms (70 -300 fold mag).
QUOTE:
There are more animals living in the
scum of a mans mouth, than there are
men in a whole kingdom? Especially in
those who dont ever clean their teeth,
whereby such a stench comes from the
mouth of many of them that you can scarce
bear to talk with them.
A.V. L. Letter 39
17 September 1683
Published in Proc. Royal. Soc. XIV, No 159, p 568 1684
Important events
1498: Chinese encyclopaedia
The modern toothbrush
Cylindrical
Rod/Bacillus
Pleiomorphic:
Spiral Actinomyces israeli
Spirillium A mixture of microbes
Helical (different colours)
(Scanning electron
Spirochete microscope, falsely
Square coloured)
Pleomorphic
Diversity of Microorganisms:
Arrangement
Determined by the plane
of cell division (binary
fission)
Rods
1 plane to produce a
single rod or chain of rods
Cocci
1 plane to produce single
cocci or diplococci
2 plane to produce a
tetrad
3 random planes to
produce a bunch
Bacteria: Binary Fission
When one bacterial cell
undergoes repeated
rounds of division on a
solid surface, it results in a
single colony composed of
identical cells
This is a very fast process
Escherichia coli
undergoes one round of
cell division in 20 min
Bacteria: Composition
WATER 70%
DRY WEIGHT 30%
Composed of:
DNA 3% (MW = 2 x 109)
RNA 12%
Protein 70%
found in:
4 6
Ribosomes (10 ) (RNA-protein particles, MW = 3 X 10 )
Enzymes
Plasmids
Circular (single or multiple copies)
supercoiled dsDNA (2-200kb) Supercoiled
Replicate independently of the Unit lengths
1000 base pair (bp) units = kilobase (kb)
host chromosome
1000 kb = 1 megabase (Mb)
Carry genes which are not Escherichia coli genome = 4.6Mb or 1,400mM
essential to the host but may be
useful in certain environments
Bacteria: Cytoplasm
Cytoplasmic matrix
Located between the plasma
membrane and the nucleoid
Composition
70% water
Contains ribosomes
Composed of
proteins/ribonucleic acid
(RNA)
Two protein subunits: 30S and
50S
Essential for protein synthesis
Highly organised cytoskeleton-
like system of proteins for cell
division
Optional: a variety of inclusion
bodies (specific to different
bacteria)
Bacteria:
Cytoplasmic membrane
Composition
Amphipathic phospholipids
organised as a bilayer
Proteins
Function
Selectively permeable
membrane that determines
transport of molecules into
and out of the cell
Nutrient transport
Energy metabolism
First step in transport of
proteins out of the cell
Bacteria: Cell Wall
Function
Maintains shape
Provides protection from
Osmotic shock
Action of anti-bacterial
agents (eg. Antibiotics,
antiseptics etc)
Contain components
contributing to pathogenicity,
in particular, inflammation
Present in all bacteria
(except Mycoplasma) and
Archaea
Procedure for the Gram strain
Characteristics of Gram positive and
negative cells
Bacteria:
Components external to the Cell Wall
Variety
Capsules and Slime Layers
Pili / Fimbriae
Flagella
Roles
Protection from the environment
Adhesion
Motility
Capsules and Slime Layers
Capsule
Organized matrix firmly
anchored to the cell
Streptococcus
Slime layer mutans
Diffuse, disorganized matrix,
easily removed
Capsule
Composition
Polysaccharides
Poly amino acid
Capsule stain of
Role Streptococcus
Resist phagocytosis lactis
Protection from desiccation
Adherence
Gliding motility (slime)
Pili / Fimbriae
Short, fine, hair-like
appendages
Hollow brittle tube
Consists of multiple pilin
subunits organized as a helix
Types I-IV defined by
Length
Diameter of the fibre
Location on the cell
Polar
Peritrichous
Single, multiple
Twitching
Function motility
Adherence
Twitching motility
Gliding motility
Myxococcus
Synechocyctis
Flagella and Motility
Hair-like appendage
10-20um long
0.02 um thick
Consists of
Hollow filament composed of
flagellin protein
Basal motor structure drives rotation
of flagellum which acts like a
propeller
Different attachment locations on
cell
Polar
Peritrichous
Mono- or multiple
Role
Swimming in aqueous environments
Seeking nutrients: chemotaxis
Alternate cell structures:
Endospores
Distribution
Produced by small number of
Terminal eg
bacteria Clostridium tetani
Bacillus, Clostridium
Structure
Subterminal eg
Bacillus subtilis
Central eg
Bacillus anthracis
Endospores: Characteristics
Property Vegetative cells Endospores
Characteristics
Resist extreme conditions Typical Gram- Thick spore coat,
positive murein cortex, and
which would kill the vegetative Surface coats
cell wall peptidoglycan
cell, for example polymer core wall
Nutrient starvation
High temperatures (100oC for Microscopic
Nonrefractile Refractile
1 hr) appearance
Salt Calcium dipicolinic
Absent Present in core
Core contains dipicolinic acid acid
(15% dry weight) Cytoplasmic water
High Very low
Sporulation occurs in response activity
to limited carbon, nitrogen,
Enzymatic activity Present Absent
phosphorous
Role Macromolecular
Present Absent
synthesis
Endospore germinates in
favourable conditions to Heat resistance Low High
become a vegetative cell Resistance to
chemicals and Low High
acids
Remove them from the
Radiation resistance Low High
environment by autoclaving
Sensitivity to
Sensitive Resistant
lysozyme
Sensitivity to dyes
Sensitive Resistant
and staining
Endospores: Sporulation
Alternate Cell structures:
Conidiospores
Distribution
Produced by
Streptomyces
Response to nutrient starvation
Structure
Thin wall spores at the end of
filaments
Characteristics
Resistant to desiccation but not
heat