Graphene is the most widely researched new material. It is an allotrope of carbon and due to special properties the material is being tested out within the fields of consumer and medicinal electronics. While these applications have received considerable attention developments relating to microbiology are taking place. This short review article considers bacterial staining and anti-bacterial activity, as two of the most promising future developments.
Graphene is the most widely researched new material. It is an allotrope of carbon and due to special properties the material is being tested out within the fields of consumer and medicinal electronics. While these applications have received considerable attention developments relating to microbiology are taking place. This short review article considers bacterial staining and anti-bacterial activity, as two of the most promising future developments.
Graphene is the most widely researched new material. It is an allotrope of carbon and due to special properties the material is being tested out within the fields of consumer and medicinal electronics. While these applications have received considerable attention developments relating to microbiology are taking place. This short review article considers bacterial staining and anti-bacterial activity, as two of the most promising future developments.
De kcal ue mel)
Introduction
Graphene is the most widely researched new material. It
is an allotrope of carbon and due to special properties the
material is being tested out within the fields of consumer
and medicinal electronics. While these applications have
received considerable attention, developments relating
to microbiology are taking place. This short review article
considers bacterial staining and anti-bacterial activity, as
‘wo of the most promising future developments.
Graphene is a material derived from carbon and it has
unique physicochemical properties. Graphene is formed
where graphite is taken and atom thick layers are sliced
away. The resultant structure isa single-layer of carbon
‘atoms linked in a hexagonal chicken-wire pattern.
Within the structure each of the atoms share a cloud of
electrons moving freely about the surface. The material
is light, transparent, strong and very conductive (Allen
et al, 2010). Compared to other carbon allotrope, such
as fullerenes, carbon nanotubes and graphite, graphene
exhibits many exceptional physical and chemical
properties. Graphene related materials are of great
interest in the field of biomedicines and applications are
underway in biosensing and drug delivery.
eee seer
eet
Graphene as a Gram-stain alternative
New research, from the University of Illinois at Chicago,
suggests that graphene can be used for performing
+ Gram-stains, as part of microbial identification. A review
of the properties of graphene shows it can detect.
variances to cell vibration when a cell comes into contact
with the material. So far the tests undertaken with
graphene have related to cancer. Here atomic vibration
differs depending upon whether the cell is a cancer cell
or a normal cell. This happens because the cancer cells
hyperactivity leads to a higher negative charge, and
this causes a higher level of protons to be released. This
difference can be detected, helping medical technologists
to identify cancerous growth,
Assessing the variances in vibration is possible using an
established laboratory method called Raman spectroscopy
{@ spectroscopic technique used to observe vibrational,
rotational, and other low-frequency modes in a system)
According to lead researcher Vikas Berry, associate
professor and head of chemical engineering, who
led the research along with Ankit Mehta, assistant
professor of clinical neurosurgery in the UIC College
‘of Medicine: “We may be able to use it with bacteria
to quickly see if the strain is Gram-positive or
Gram-negative...WWe may be able to use it to detect
sickle cells.” Berry made this remark to Controlled
Environments magazine (Anon, 2017).
Ina parallel development, one research group have
created a graphene sensor for Escherichia coli. This
involved fabricating a flexible substrate onto which a
sensor device with O-ring is fitted. Once contact takes
place with the suspected organism, Raman spectra is
used to indicate the presence (Basu et al, 2014).
eee
‘As all microbiologists know, the Gramstain is the key test
{or distinguishing between two groups of bacteria based
cn cell wall morphologies (Sandle, 2014). The Gram
stain procedure distinguishes between Gram positive
and Gram negative groups by coloring these cells red
or violet. Gram staining is a common technique used to
differentiate two large groups of bacteria based on their
different cell wall constituents. The Gram stain procedure
distinguishes between Gram positive and Gram negative
‘groups by coloring these cells red or violet. Gram positive
bacteria stain violet due to the presence of a thick layer of
peptidoglycan in their cell walls, which retains the crystal
violet these cells are stained with. Alternatively, Gram
negative bacteria stain red, which is attributed to a thinner
peptidoglycan wall, which does not retain the crystal violet
during the decolouring process (Sandle, 2004)
————__<_<_—_———_—While the Gram-stain technique is well described it
is sometimes prone to error. This can relate to the
‘types of organisms, the age of the cultures, or due to
errors made by the person performing the test (such
as over decoloursation). A method based on graphene
would error proof. Whether such a method becomes
commercially available will depend on development costs
Graphene as an antibacterial agent
‘As well as using graphene as a potential diagnostic tool,
graphene can also be used as an anti-bacterial measure
(where liposome-embedded graphene reduces the
‘growth capabilty of bacteria). Research by Zappacosta
and colleagues showed that graphene aqueous
dispersion is stable for several days and demonstrates
significant antibacterial activity against both Gram-positive
(Staphylococcus aureus) and Gram-negative (Escherichia
oll) strains, with a reduction in the growth of S. aureus
and E. coli as high as 60 and 78%, respectively.
Ina similar application, researchers have looked
at graphene-iodine nano-composites, formed
via electrostatic interactions between positively
charged graphene derivatives and triiodide anions,
as anti-bacterial agents (Some et al, 2015). With
this, the antibacterial potential of these graphene-
iodine composites against Klebsiella pneumonia,
Pseudomonas aeruginosa, Proteus mirobilis,
Staphylococcus aureus, and Escherichia coli has been
demonstrated. The success against the organisms relates to
the inherent cytotoxicity of the nanocomposite, specifically
through electron transfer interaction from microbial
membrane to graphene,
Image: Futuristic
Furthermore, scientists are studying graphene oxide
with the aim of creating bacteria-kiling catheters and
medical devices. Here coating surgical tools with this
carbon-based compound could kill bacteria, reducing
the need for antibiotics, decreasing the rates of post-
‘operative infections and speeding recovery times. This is
with graphene oxide, which is a form of graphene with
molecular oxygen incorporated into it. This compound
protects against infection by destroying bacteria before
it gets inside the body. In terms of the process the
‘graphene oxide wraps around the bacteria, puncturing
its membrane. A broken membrane prevents the bacteria
from growing and often kil it.
Studies conducted at the Universita Cattolica del Sacro
Cuore in Rome indicate that the compound is most
effective when paired with salt. Getting the salt balance
cortect is important. With too littl salt and then the
graphene oxide is unable to wrap around the bacteria;
and with too much salt and the graphene aggregates,
failing to puncture the bacteria's membrane. In order to»
destroy both Gram-postive and Gram-negative bacteria
‘2.300 nanometer sheet of graphene oxide solution must
be mixed with low molarity (<10 mM) calcium chloride is
required (Anon, 2015).
Summary
These two related research strands (for differential
microbiology and as an antibacterial agent) signal that
graphene, the so-called ‘wonder material’ of our age, is
set to make a significant impact upon microbiology. As
with the development of any novel method, progress
will be slow. However, the research results reported
to date suggest that graphene is set to make a major
contribution to microbiology.
References
1. Allen, M. J., Tung, V.C. and R. B. Kaner, R.B.
Honeycomb carbon: a review of graphene, Chem. Rev.
2010, 110, 132
2. Anon. Biophysical Society report “Towards a “green”
antimicrobial therapy: Study of graphene nanosheets
interaction with human pathogens”, 2015 (http/
tinyurl.comizzgsofu)
3. Anon. First Use of Graphene to Find Cancer Cells,
Controlled Environments, 2017 (httpifwww.cemag.us!
rnews/2017/02/irst-use-graphene-find-cancer-cells)
4, Basu, PK., Indukuri, D., Keshavan, S., Bhat, N.
(2014) Graphene based E. coli sensor on flexible
acetate sheet, Sensors and Actuators 8 Chemical
190:342-347 (https:/Awww.researchgate.ney
publicatior/256926101_Graphene_based_E_coli_
sensor_on_flexible_acetate_sheet)
5, Sandle, T. 2004) Gram’s Stain: History and Explanation
of the Fundamental Technique of Determinative
Bacteriology’, IST Science and Technology, No. 54, pp3-4
6. Sandle, T. (2014). ‘Microbial Identification: Laboratory
Techniques and Methods. In Chesca, A. (Ed.) Methods
or Diseases: Diagnostic with Applicability in Practice,
Lambert Academic Publishing, Germany, pp15-26
7. Some, S., Schm, J., Kim, J. et al Graphene-lodine
Nanocomposites: Highly Potent Bacterial Inhibitors that
are Bio-compatible with Human Cells, Scientific Reports
6, Article number: 20015 (2016). doi:10.1038/srep20015
8. Zappacosta, R., Di Giulio, M., Ettorre, V, et al
Liposome-induced exfoliation of graphite to few-layer
Digital Transformation and Regulatory Considerations For Biopharmaceutical and Healthcare Manufacturers Volume 1 Digital Technologies For Automation and Process Improvement