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Francisco LASO
Erasmus Mundus Masters Programme in
Evolutionary Biology
Supervisors:
Pr. Marc-Andre SELOSSE
& Dr. Lucie VINCENOT
Ecologically, economically
important, phylogeography
remains understudied
Intercontinental species in
microbes : Everything is
everywhere - null hypothesis
or fact ?
The continental ECM
species are based on
morphology
Laccaria amethystina as a
model
!
339 samples 7 neutral
microsatellite
12 pops loci
Nuclear
19-35 fruit-
rDNA ITS
bodies/pop
sequence
n=118
n=153 n=68
Main results
1) 3 gene pools
4 6
Shared alleles
13 12
9
2
Correspondence factorial analysis
K=3
(b)$
K=4
8,770km
Fst = 0.52
Inter-regional Mantel tests
P = 0.001
P = 0.0095
Distinct outgrowths in
Chinese population as
compared to Europe
(at least).
3 species?
Low marker portability in preliminary tests
High Fst values between regions
CFA and STRUCTURE results consistent
No pattern suggesting gene flow between
regions
Different microscopic features
0.99
China
0.70
Phylogeny
Japan
0.83
1.00
0.99
L. bicolor
0.83
Europe
L. amethystina
China-Japan
Genetix 4.05.2 results
GENETIX 4.05.2
! (a)$
K=3
(b)$
K=4
STRUCTURE 2.3.3
GENELAND