Professional Documents
Culture Documents
Saccharomyces cerevisiae
objectives
- metabolic fates of pyruvate
- krebs cyvce
- aerobic metabolism
-
fructose decolorizes more quickly than glucose. skips isomerization part into
fructose. next, PFK to F-1,6-P. rate of decolorization differs, but the color
should be the same if left for a while.
37 > 25 > 60
in the long run, sucrose has biggest balloon. it has both gluc and fruc
units.
EFFECT OF INHIBITORS
sodium fluoride
fluoride ion sequesters Mg2+
formation constant of MgF2 is higher. aagawin yung Mg2+ from
the enzyme. (many enzymes are metalloenzymes)
sodium citrate
- inhibits PFK (phosphofructokinase)
o F-6P[PFK] F-1,6-BP COMMITTED STEP. tuluy-tuloy na sa
glycolysis at this point.
o allosteric regulator.
sodium citrate attaches to PFK; PFK can no longer
phosphorylate F-6P