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Medium introducted by ZEBOVITZ et al. (1955) and modified by VOGEL and JOHNSON
(1960) for the detection of mannitol-positive staphylococci in clinical specimens and other
materials.
This culture medium complies with the recommendations of the United States Pharmakopeia
XXIII (1995).
Mode of Action
The growth of accompanying bacteria is almost completely inhibited by tellurite, lithium
chloride and a high glycine concentration. The staphylococci may also be slightly inhibited,
but this is compensated by the presence of mannitol and glycine. Mannitol also serves as a
differentiation reactant as it is degraded to acid by most pathogenic staphylococci; this
reaction is indicated by phenol red, which changes its colour to yellow. Pathogenic
staphylococci also reduce tellurite to metallic tellurium, thus their colonies turn black in
colour.
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Preparation
Suspend 58 g/litre and let stand for 30 minutes. During dissolving, agitate frequently (aprox.
every 5 min). Swirl once more prior to autoclaving. Autoclave (15 min at 121 °C). Prior to use,
add 0.24 g potassium tellurite/litre in the form of a filter-sterilized solution at a temperature of
about 50 °C, mix. Pour plates.
n The complete culture medium can be stored in the refrigerator for up to 1 week, whereas
the prepared culture medium base can be kept for several months.
Additives
Quality control
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Literature
Staphylococcus aureus
© 2000 Merck KGaA, Darmstadt, Germany
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