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Day 1: Zygote
Fertilization begins when sperm
penetrates ZP (zona pellucida -
membrane of oocyte)
Fusion of oocyte and sperm nuclei
begins formation of zygote
Clay Model of Embryogenesis
Day 4: Morula
Early embryo stage: consists of 16-32
cells now called a morula
After 16 cells, the cells of the morula
begin to differentiate
Clay Model of Embryogenesis
Day 5: Blastula
A spherical layer of about 120 cells
(trophoblast) surrounding a
fluid-filled cavity (blastocoel) forms
blastula
Two primary cell lines differentiate
into inner cell mass (epiblast) and
outer trophoblast
Trophoblast secretes an enzyme that
erodes inner lining of uterus (for
implantation)
Clay Model of Embryogenesis
2. If an embryo entered the uterus at the morula stage would it implant successfully into the uterine wall? Explain your answer.
No, because the morula still has the ZD (zona pellucida membrane). The cells of the morula have not differentiated to form the
trophoblast cells, which secrete the enzyme required to erode the lining of the uterus for implantation.
4. At what stage of embryonic development are stem cells used to generate embryonic stem cell lines. Which type of stem cells are
found at this stage?
Blastula, pluripotent
5. What Type of tissue is formed from the following primary germ layers?
1. Endoderm - most internal layer. Forms internal organs, lungs and digestive system
2. Ectoderm - most exterior. Forms skin, brain, nervous system
3. Mesoderm - middle layer. Forms circulatory systems, bone, muscle, connective tissue