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OBJECTIVE
INTRODUCTION
PROCEDURE
RESULTS
Result Description
UV
-Examine under 312
nm UV light
-no reaction for all
sample
Fluorescence
-5 sample were
separated for sample
2
-6 sample were
separated for sample
3
Result Description
UV
-Examine under 312
nm UV light
-no reaction for all
sample
Fluorescence
-5 sample were
separated for sample
medicine 2
-4 sample were
separated for sample
medicine 3
Spray with 10% sodium hydroxide solution and 1 % methanolic tetrazolium
blue solution
Result Description
Mobile phase A
-Reference sample
turns to purple color
-Sample 1 turns to
yellow color
-No color change for
sample 2 and sample
3
Mobile phase B
-Sample 1, sample 2
and sample 3 turns to
yellow color
-No color change for
reference sample
DISCUSSION
Both of the TLC plate with mobile phase A and mobile phase B were
sprayed with 10% sodium hydroxide solution and 1 % methanolic
tetrazolium blue solution which to look up their sensitivity, selectivity and
specificity. In TLC plate with mobile phase A the reference sample showed
the purple color and yellow color for sample 1. Both sample 2 and 3 did
not showed any color changed. Meanwhile, in TLC with mobile phase B,
only sample 1 showed the color change which is yellow color and the other
three samples did not showed any color change.
Thin layer chromatography (TLC) is a chromatographic technique used to
separate the components of a mixture using a thin stationary phase
supported by an inert backing. It may be performed on the analytical scale
as a means of monitoring the progress of a reaction, or on the preparative
scale to purify small amounts of a compound. TLC is an analytical tool
widely used because of its simplicity, relative low cost, high sensitivity and
speed of separation.
Steroid also can be detected and analyzed by GC-MS apart from TLC. Many
commercial GC-MS instrument can provide the ultimate speed, accuracy
and sensitivity. With the ability to record and store in its memory several
hundred mass spectra, such a system can detect and identify substances
present in only one-million-of-a-gram quantities. Furthermore, the
computer can be programmed to compare an unknown spectrum against a
comprehensive library of mass spectra stored in its memory. Therefore,
the retention time of the steroids peak and its mass spectrum may give an
absolute identification of steroids.
REFERENCE