Professional Documents
Culture Documents
Uses
Muller-Kauffmann Broth Base with Brilliant Green & Novobiocine is recommended by the ISO 6579:2002 norm to
be used a selective enrichment broth of Salmonella spp. Calcium carbonate is a neutralizer which absorbs toxic
metabolites. The Bile Salts and the Brilliant Green inhibit gram positive, and most gram negative, bacterial growth
Microorganisms with tetrathionate reductase enzyme will grow and multiply in this medium.
Bibliography
Kauffmann, F. 1935. Weitere erfahrungen mit dem kombininierten anreicherungsverfahren fur Salmonella bazillen.
Ztschr. F. Hyg. 117: 26-32.
A manual for recommended methods for the microbiological examination of poultry and poultry products.
1982. ISO 6579:2002.
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature
of 37 1 C, and observed after 24 3 hours.