World Academy of Science, Engineering and Technology

International Journal of Pharmacological and Pharmaceutical Sciences Vol:1, No:11, 2014

Phytochemical Study and Evaluation of the Antioxidant Activity of Flavonoids

Isolated from Prunus persica L. Leaves
Authors : K. Fellah, H. Benmehdi, A. Amrouche, H. Malainine, F. Memmou, H. Dalile, W. Siata
Abstract : This work aims to evaluate the antioxidant of flavonoids extracted from the leaves of Prunus persica L. A
phytochemical screening allowed us to highlight the different phytochemicals present in the leaves of the studied plant. The
International Science Index, Pharmacological and Pharmaceutical Sciences Vol:1, No:11, 2014 waset.org/abstracts/13223

selective extraction of flavonoids gave yields of 0.71, 1.5, and 4.8% for the fractions ethyl ether, ethyl acetate and n- butanol,
respectively. The reading of the antioxidant activity of different extracts of flavonoids by HPLTC method revealed positive
reaction (yellow spots) on the TLC plates sprayed with DPPH. Using the DPPH method, the fractions of flavonoids (bunanol,
ethyl acetate and Diethyl ether) showed a potent scavenging activity with IC50 = 0.22; 0.27 and 0.76 mg / ml, respectively.
Furthermore, our findings revealed the extracts under study exhibited higher reducing potential which depends upon extract
concentration. These results obtained from this investigation confirm that the Prunus persica remains a major resource of
bioactive molecules.
Keywords : Prunus persica L., phytochemical study, flavonoids, antioxidant activity, TLC bioautographic, FRAP, DPPH
Conference Title : ICPPNP 2014 : 16th International Conference on Pharmacognosy, Phytochemistry and Natural Products
Conference Location : Istanbul, Turkey
Conference Dates : November 28-29, 2014

International Scholarly and Scientific Research & Innovation 1(11) 2014 164 scholar.waset.org/1999.52/13223