Professional Documents
Culture Documents
CHAPTER: 3
Bacterial Growth Kinetics
1
Microbial Growth Kinetics
rg .x
rg = net biomass production rate (g VSS/m3.d)
rate of increase in bacterial g VSS
pop. is proportional to the = specific biomass growth rate
number of bacteria present g VSS.d
g VSS
Autocatalytic rxn X = biomass concentration
aA bB r=k[B] m3
g new cells
Growth rate of bacteria can be defined by Monod eqn
g cells.d
2
S
where = specific biomass growth rate
mK S
s
M= max specific biomass growth rate (g VSS/g VSS.d)
3
Specific growth rate ()
M
2
S
For high substrate conc (S>>Ks) mK S
s
negligible with respect to S
= M
M
2
rg
r biomass productionrate (g VSS/m3d) Y
g rsu
r substrate utilization rate (g subst/m3d) rg Yrsu
su
(-) substrate is decreasing with time due to substrate utilization
r mXS
g m XS
Ks S r
su Y Ks S
rg Yrsu
k= max specific substrate utilization rate
6
Substrate utilization rate (mg/Ld)
k
k
2
kXS
For high substrate conc (S>>Ks) rsu = -k.X
Ks S
7
Substrate utilization rate (mg/Ld)
k
k
2
1.the energy required for cell maintenance m.o in all growth phases require
energy for cell maintenance
2.cell death in bacterial systems used for ww
treatment not all the cells in the
system are in log growth phase
Endogenous decay coeff rate (time-1) accounts for the cell mass due to oxidation
of internal storage products for energy for
cell maintenance, cell death
The decrease in cell mass caused by energy requirement for cell maintenance and
cell death is proportional to the concentration of m.o present
9
The endogenous decay term can be formulated as follows:
rg mXS
Ks S rg' mXS k X
Ks S d
rd kdX
net rate of bacterial
growth (g VSS/m3d)
10
' mXS k X
XS rg Ks S d mS k
rg' m kdX '
Ks S X X Ks S d
not specific growth rate, time-1
rg X
rg rg'
Y Yobs
rsu rsu
11
Effect of Temperature
Effect of temperature on the rxn rate of a biological process is expressed as follows
12
Total Volatile Suspended Solids and Active Biomass
active biomass
cell debris
following endogenous decay
13
During cell death cell lysis occurs
Determine ;
values
* Adapted from Henze et al. (1987a); Barker and Dold (1997); and Grady et al. (1999)