Total Bilirubin (TB) Reagents Kit 2.

Incomplete mixture of specimen and reagents results in

APPLICABLE INSTUMENT inaccurate assay. Always mix thoroughly before assay.
(Vanadate Method) For full-automated biochemistry analyzer. 3. All pipettes and instruments should be calibrated before
use.
4. To ensure the accuracy of results, reaction temperature and
SPECIMEN time period should be controlled strictly in assay.
5. Reagents storage and validity please see the package insert.
Fresh serum, or plasma. 6. Please refer to the local legal requirements for waste
management.
PROCEDURES 7. This kit is for professionals and in vitro diagnostic use only.
INTENDED USE
Test Mode: two end point method;
For the quantitative determination of total bilirubin in human Temperature: 37;
serum and plasma Cuvette: 1.0cm;
Wavelength: 450nm;
Specimen: 10 ul;
Specification R-1: 280, R-2: 70ul;
Reaction: down
150ml/box R-160ml2; R-215ml2
Test step
Addition Blank tube Standard tube Test tube
PRINCIPAL Distilled water 7 L
Under the particular term pH3.0 Bilirubin can react with Standard 7 L
Sample 7 L
vanadic acid to form biliverdine in serumThen the absorbance of R-1 210 L 210 L 210 L
bilirubin at 450nm decreases and is proportional to concentration in Mixing and incubate for 3-5mins under 37test each
Tube A2A=A2A1
blood serum.. Therefore the total bilirubin concentration in the R-2 70 L 70L 70 L
sample can be obtained by measuring the absorbance before and
CALCULATIONS
after the vanadate oxidation. A specific
T.Bilimol/L= Standard Conc.
Vanadic Acid A standard
Bilirubin Biliverdin
REFERENCE RANGE
5.020.1mol/L0.31.2mg/dl
It is suggested that each lab establish individual reference
REAGENTS range.
Component Conc.
PRODUCT PERFORMANCE
R1 Citrate buffer 100 mmol/L 1. Precision: Within run: CV4.0; Inter run: CV5.0
R2 Sodium metavanadate 10 mmol/L 2. Linearity: 0684mol/L

PRECAUTIONS
STORAGE 1. Can change the specimen and reagent volume based on
Stable for 24 months, direct exposure to sunshine avoided. analyzer requirement.

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