OASIS LABORATORIES PRIVATE LIMITED, DEHRADUN

SOP No.:
QUALITY CONTROL DEPARTMENT Page 1 of 3
QCG001
Supersedes : Effective date:
STANDARD OPERATING PROCEDURE
00 April 2010
Version No. : SOP FOR OPERATION AND CALIBRATION Review date:
01 OF REFRACTOMETER March 2013

1.0 OBJECTIVE:

To describe the procedure for the Operation and Calibration of

Refractometer.

2.0 SCOPE:

This SOP is applicable to Operation and Calibration of Refractometer at

M/s. Oasis Laboratories Private Limited, Dehradun.

3.0 RESPONSIBILITY:

Quality Control Department

4.0 ACCOUNTABILITY:

HOD QC

5.0 PROCEDURE :
5.1 Open the prism by unlocking the lever, place of few drops of the
liquid under study on the surface of the lower prism, which has a
matt and ground surface.

5.2 Lift the lower prism and lock it with the upper prism by the same
lever when the liquid will be formed into a very thin layer between
the two prisms.

5.3 Look through the telescope when a bright circular path of the light
partly coloured will be seen.

5.4 Focus the eyepiece on the index line and adjust the reflecting
mirror to get a maximum illumination through the telescope.

5.5 Rotate the milled drum in front till a coloured band of light
appears.

5.6 Rotate the milled drum till this sharp critical line of demarcation
coincides with the junction of the crossed graticulas index lines.

Designation Signature Date

Prepared by Chemist QC
Reviewed by Manager QC
Approved by Manager - QA
 OASIS LABORATORIES PRIVATE LIMITED, DEHRADUN
SOP No.:
QUALITY CONTROL DEPARTMENT Page 2 of 3
QCG001
Supersedes : Effective date:
STANDARD OPERATING PROCEDURE
00 April 2010
Version No. : SOP FOR OPERATION AND CALIBRATION Review date:
01 OF REFRACTOMETER March 2013

5.7 Look though the left eye piece and focus the lens till the refractive
index scale and the graticulas index line are in clear focus, take
the reading of the scale, this gives the refractive index of the
sample.

5.8 Repeat the process to take a number of readings whose average

gives the refractive index of the liquid.

5.9 It the refractive index is to be determining at a fixed desired

temperature, water from a separate thermostatic bath be
circulated within the prism box using the inlet and outlet tubes and
check the temp. with thermometer.

5.10 Before putting in any new liquid, the surface of the prism must be
thoroughly cleaned with water or other organic solvent like
acetone to remove the solution previously us.

5.11 Before keeping the instrument in the wooden box, the prisms
must be thoroughly cleaned and dried.

6.0 CALIBRATION:
To achieve accuracy, the apparatus should be calibrated against distilled
water which has refractive index of 1.3325 at 25 or against the
reference liquids given are below:-

Reference liquid Refractive Index at 20

Carbon tetrachloride 1.4603
Toluene 1.4969

Frequency of calibration : Monthly

7.0 RECORDS:

Calibration Record of Refractometer (Annexure-1).

8.0 ANNEXURES:
Annexure-1: Format for Calibration Record of Refractometer.
9.0 ABBREVIATIONS & DEFINITIONS:
SOP : Standard Operating Procedure.
Dept. : Department.

Designation Signature Date

Prepared by Chemist QC
Reviewed by Manager QC
Approved by Manager - QA
 OASIS LABORATORIES PRIVATE LIMITED, DEHRADUN
SOP No.:
QUALITY CONTROL DEPARTMENT Page 3 of 3
QCG001
Supersedes : Effective date:
STANDARD OPERATING PROCEDURE
00 April 2010
Version No. : SOP FOR OPERATION AND CALIBRATION Review date:
01 OF REFRACTOMETER March 2013

HOD : Head of the Department.

QC : Quality Control
ANNEXURE: 1

SOP REF NO QCG001 QC-F-001-01

Name of Instruments : Refractometer Apparatus

Name of Manufacturer : Delhi India
Instrument ID Number : QC/INS/010
Frequency of calibration : Monthly
Instruments location : Q.C. LAB.

CALIBRATION PROCEDURE: To active accuracy of the instrument it calibrated against water which
has refractive index of 1.3325 at 250 C against following liquid.

Date Reference Liquid std R.I . Observed Tolerance Next Due Remark Performed Checked
at 200 c R.I. Date by by
Carbon Tetrachloride 1.4603 0.0001
Toluene 1.4969 0.0001

Designation Signature Date

Prepared by Chemist QC
Reviewed by Manager QC
Approved by Manager - QA