Application Note

No. 630002H-E

Analysis of Aflatoxins (1)

Aflatoxins are mold venom generated from Aspergillus Condition

flavus and about ten kinds of the related compounds are
known. It is considered that Aflatoxins B1, B2, G1, G2 and Column: Finepak SIL-5
M1 can be carcinogens and contaminants for foods. Eluent: Toluen / Ethyl Acetate / MeOH /
According to a hygienic method, five kinds of Aflatoxin Formic acid = 50 / 5 / 2 / 1
s ta n d a rd m i x tur e we r e s e p ar ated by n or m a l p ha s e Wavelength: Ex 365 nm, Em 425 nm, Gain x 100
chromatography and detected with a fluorescence detector. Flow rate: 1.0 mL/min.
Figure 1 shows the chromatogram of Nuts pretreated Column temperature: 40C
according to the procedure shown in Figure 2. Injection volume: 10 L
Sample: STD mixture
B1: 0.8 ppm
B2: 0.4 ppm
Keywords: 1. Aflatoxins, 2. STD mixture 3. Nuts G1: 0.4 ppm
4. SIL, 5. FL G2: 0.4 ppm
M1: 0.6 ppm

Fig. 2 Pretreatment method

Fig. 1 Chromatogram of Aflatoxins standard mixture and Nuts

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