1.

Transmitted via electromagnetic waves that are

characterized by their frequency and wavelength
2. It is the distance between two successive peaks
3. It is the unit used in expressing wavelength
4. The formula that describes the relationship between the
wavelength and energy
5. The formula used in the answer in question number 4
6. It is the number of vibrations of wave motion per second
7. Relation of the wavelength to frequency and energy
8. The ____ the wavelength , the higher the
frequency/energy
9. It represents the wavelength In nanometers at peak
transmittance
10. It is the wavelength indicated on the control dial
11. Actual wavelength of light passed on the
monochromator
12. Used to check wavelength accuracy (wavelength
calibration)
13. Verify absorbance accuracy on linearity
14. Is the isolation of discreet portions of the spectrum for
purposes of measurement
15. Measurement of light intensity in a narrower
wavelength
16. Measurement of light intensity
17. Involves measurement of the light transmitted by a
solution to determine the concentration of the light
absorbing substances in the solution
18. It is the simplest type of absorption
spectrophotometer
19. It splits the monochromatic light into two components-
one beam passes through the sample and the other
through a reference solution or blank
20. It is designed to make one measurement at one
specified wavelength
21. What must be known in advance when a single beam
instrument is used
22. It corrects the variation in light source intensity
23. The absorbance of the sample can be directly recorded
as the electrical output of the sample beam
24. Type of double beam spectrophotometer that uses 2
photodetectors, for the sample beam and reference
beam
25. It uses one photodetectors and alternately passes the
monochromatic light through the sample cuvet and then
reference cuvet using a chopper or rotating sector mirror
26. It provides polychromatic light and must generate
sufficient radiant energy or power to measure the analyte
of interest
27. What is directed through the monochromator and the
sample?
28. The response to change in light must be ___ to give
accurate absorbance measurements throughout its
absorbance angles
29. It emits radiation that changes in intensity, it is widely
used in the laboratory
30. It is the commonly used light source in the visible and
near infrared region
31. It is routinely used to provide UV radiation in analytic
spectrometers
32. It produces a continuous source of radiation which
covers both the UV and the visible range
33. It emits limited radiation and wavelength it finds wide
use in atomic absorption molecular and fluorescent
spectroscopy
34. It is also used as a light sources for spectrophotometry
35. It minimizes unwanted or stray light and prevents the
entrance of scattered light into the monochromator
system
36. Refers to any wavelength outside the bad transmitted
by the monochromator; does not originate from the
polychromatic light source
37. It causes absorbance error
38. It is the most common cause of loss of linearity at high
analyte concentration and it limits the maximum
absorbance that a spectrophotometer can achieve
39. It isolates specific or individual wavelength of light
40. Are wedge-shaped pieces of glass, glass or sodium
chloride
41. It is most commonly used and has a better resolution
than a prism
42. Are simple monochromators that are least expensive
and not precise but useful
43. It is made by cutting grooves or slits into an aluminized
surface of a flat piece of crown glass
44. It produce monochromatic light based on the principle
of constructive interference of waves
45. Kind of monochromator that can be rotated, allowing
only the desired wavelength to pass through an exit slit.
46. What is refracted on a prism as it enter the more
dense glass
47. It controls the width of light beam
48. It is the total range of wavelengths transmitted
49. It requires a bandpass less than 1/5 the natural
bandpass of the spectrophotometer
50. A function of the type of device used and the width of
entrance and exit slits
51. It is reflected by the bandpass that is the narrower the
bandpass the greater the resolution.
52. Is called absorption cell/ analytical cell/ sample cell
53. Holds the solution whose concentration is to be
measured.
54. It is a kind of cuvet that is most commonly used and
can be used in 350-2000 nm
55. Used for measurement of solution requiring visible and
ultraviolet spectra
56. It scatters light and should be discarded
57. Transmit light effectively at wavelengths >220nm
58. Path lengths of cuvet
59. Length given to cuvettes to increase sensitivity
60. It detects and converts transmitted light into
photoelectric energy and detects the amount of light that
passes through the sample in a cuvette
61. It is the simplest least expensive and a temperature
sensitive kind of detector
62. What kind of detector is used in filter photometers
with a wide bandpass and used for detecting and
measuring radiation in the visible region
63. It contains cathode and anode enclosed in a glass case
64. It is the most commonly used detector that measures
visible and UV regions
65. It requires an external voltage for operation and has a
photosensitive material that gives off electron when light
energy strikes it
66. It has an excellent sensitivity and has a rapid response
and should never be exposed to room light
67. It is not as sensitive as PMT but with excellent linearity
and measures light at a multitude of wavelengths
68. Most useful as a simultaneous multichannel detector
69. It displays output of the detection system
70. It states that the concentration of the unknown
substance is directly proportional to the absorbed light
and inversely proportional to the amount of transmitted
light
71. It is the amount of light absorbed
72. Ratio of the radiant energy transmitted divided by the
radiant energy incident on the sample
73. Formula to get the percent transmittance
74. This means that the blank contains serum but without
the reagent to compete the assay
75. It corrects absorbance caused by the color of the
reagents
76. Measures the absorbance of the sample and reagent in
the absence of the end product
77. Used to correct for artifactal absorbance readings
78. Measures the light emitted by a single atom burned in
flame
79. Light source of FEP
80. Indicates changes in the fuel reading of the instrument
81. Corrects variations in flame and atomizer
characteristics
82. It measures the light absorbed atoms disassociated by
heat
83. Used for measurement of excited ions
84. Light source of AAS
85. Used to convert ions to atoms
86. Used to modulate the light source
87. Meaning of EDTA
88. Used in chloride test
89. Used in calcium test
90. The unknown sample is made to react with known
solution in presence of an indicator
91. It is used for measuring abundant large particles
proteins and bacterial suspensions
92. Used to detect bacterial growth in broth cultures
93. Used to detect clot formation
94. Used for measuring amount of antigen antibody
complexes
95. It is the migration of charged particles in an electric
field
96. It separates proteins based on their electric charge
densities
97. Determine the net charge on a protein, hence its
electrophoretic mobility
98. Has a net charge that can be either positive or negative
depending on pH conditions
99. Is the movement of buffer ions and solvent relative to
the fixed support
100. Migration of small charged ions
101. Migration of charged macromolecules
102. Separates by molecular size
103. Separats by electrical charge and does not bind protein
104. Separates on the basis of charge and molecular size;
separates proteins into 20 fractions and is used to study
isoenzymes
105. Movement of buffer and solvent relative to their fixed
support
106. Separating molecules migrate through a pH gradient
107. Separation is formed in narrow bore fuse silica
capillaries
108. Measures the absorbance of stain concentration of dye
and protein fraction
109. It scans and quantitates electrophoretic pattern
110. Separates molecules by migration through a pH
gradient
111. Is ideal for separating proteins of identical sizes but
with different net charges
112. In this method sample molecules are separated by
electro-osmotic flow
113. Synthesize DNA