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ABSTRACT
The study was aimed at assessing the levels of some toxic metals of lead and cadmium in selected
lipstick products sold in Padang city. Four brands of lipsticks were taken which were BL, NK, PS and
WD. The lipsticks were digested and analyzed for heavy metals (lead and cadmium) using atomic
absorption spectrophotometry. Each sample was destructed by nitric acid and perchloric acid (3:1).
Destructed samples were added with sodium hydroxide to liberate ammonia and filtered into a 25
mL volumetric flask. This method was validated (linearity, detection limits, quantification limits) by
using range concentration 0.1-0.5 mg/L for Cadmium and 0.2-1.0 mg/L for Lead. In this study,
regression equations of absorbance were obtained which were y=0.0374x+0.001 with r=0.9996 for
metal Lead and y= 0.348x-0.007 with r=0.996 for metal cadmium. Detection limits and quantification
limits were calculated which were 0.0306 mg/kg and 0.1022 mg/kg for metal cadmium and 0.0462
mg/kg and 0.1542 mg/kg for metal lead. The results showed that lead heavy metal contamination
was not detected. Whereas, the heavy metal contamination of cadmium in lipstick brands BL, NK, PS
and WD were 0.2287, 0.2000, 0.1796 and 0.1220 mg/kg, respectively. The study results showed that
all metal contaminations of lead and cadmium were not over the limit which were regulated by
National Agency of Drug and Food Control of the Republic of Indonesia.
Key Words: Atomic Absorption Spectrophotometry; Cadmium; Lipstick; Lead.
INTRODUCTION
A cosmetic is a substance or preparation used in contact with various parts of the human body such
as epidermis, hair, nails, lips and external genital organs (external parts) or applied to the teeth or
mucous membranes of the oral cavity with a view or for the purpose of cleaning, perfuming,
protection, changing their outlook,converting body odours and keeping the surfaces in good
condition [1,2]. Cosmetics are mixtures of some ingredients such as surfac-tants, oils and they need
to be effective, long lasting, stable and safe to human use [3]. The various forms of cosmetic include
powder, rouge (used to colour the face, lighten and remove flaws to produce an impression of youth
and health), lipstick and lip gloss (used to colour the lips), mascara (used to enhance the eye lashes),
nail polish (used to colourthe fingernails and toenails), eye liner and eye shadow (used tocolour the
eye lids) [2].
Among the cosmetic products, lipsticks have the higher risk of direct oral ingestion, aggravating the
negative effects of their ingredients. Therefore, peoples concern about cosmetics toxicity has
become an important issue. The early lipsticks presented in the market consist of beeswax, fat and
pigment as the main ingredients. The worldwide use of lipsticks has been increasing due to the
willing of individual beautification [4]. Lipsticks are usually contain heavy metals such as lead and
cadmium [5]. Lip products are presented in a wide variety of colors, that are produced by addition of
pigments. These pigments may be mineral or organic and may contain heavy metals as impurities in
the pigment formulation [6,7]. It is believed that at certain specified limits, some heavy metals could
be of biological importance to human [8].
However, heavy metals such as lead and cadmium have been reported not to have any known
biological importance and can be very toxic even at very low concentration [9]. Cadmium is toxic at
very low levels. Exposures to the cadmium may cause renal dysfunction and long term exposure to
cadmium can lead to obstructive lung disease [10]. Cadmium is one of the heavy metals that if it is
directly subjected to the human body it would decrease the blood pressure [11]. It is also connected
with the diabet [12]. Absorption of lead by the body causes inhibition of haemoglobin synthesis,
kidney dysfunction, reproductive and cardiovascular systems dysfunction [13]. The toxic effects of
cadmium and lead are shown via bonding to sulfydryl groups of proteins and depletion of
glutathione. Lead is described as one of the most dangerous contaminants to arise in human
civilization due to its distribution in environment as polluting element [14]. Exposure of lead at high
levels is well known to be toxic, but exposure to relatively low levels may entail adverse health effect.
High levels of lead exposure may cause serious health damages including both acute and chronic
poising, pathological change of organs and disease related to cardiovascular, kidney, bone, and liver
and it can even cause cancer owing to excessive accumulation in human body [15]. Cobalt may
undergo cycling reactions to generate reactive radicals in the body [16]. In addition, Co is well known
that causing of allergic contact dermatitis [17]. Therefore, it is very important to determine the
concentration of these metals in lipsticks, which are used by millions of people. Due to wide use of
lipsticks in Padang, West Sumatera, Indonesia. The limit of heavy metals concentration which was
regulated by National Agency of Drug and Food Control of the Republic of Indonesia were 20 mg / kg
for lead and 5 mg / kg for cadmium. lack of data on their toxicity or their safety, the goal of this study
was to assess the public health risk from certain brands of lipsticks sold at Ramayana and Raya
market in Padang. In this context, four different brands of lipsticks were selected from local markets
in Padang, West Sumatera, Indonesia and the concentration of heavy metals such as cadmium and
lead were analyzed using Atomic Absorption Spectrometry.
From this study, the linear regressions of stock solutions (Cd and Pb) were obtained, which were
showed in figure 1 and figure 2 and the other datas were showed in table 1.
Information on the brand of lipstick used for the study is presented in table 2.
Table 2. Brands of lipstick used for study
Metal Pb Metal Cd
0.05 0.2
y = 0,0374x + 0,001 y = 0,348x - 0,007
0.04 R = 0,9996 R = 0,996
absorban (A)
Absorban (A)
0.15
0.03
Series1 0.1 absorban
0.02
0.01 Linear 0.05 Linear
(Series1) (absorban)
0 0
0 1 2 0 0.2 0.4 0.6
Concentration (ppm) Concentrasi (ppm)
The contents of cadmium and lead in lipstick samples are presented in table 3. As it is clear from
these tables, all the samples are negative from Pb ion. Concentrations of Pb in samples were not
detetected, may be caused all the samples were not containing Pb ion or fewer concentration of ion
Pb in samples.Then, all the samples contain variable concentration Cd ion, some within the
acceptable limits and some out of the safe range. The limited value for cadmium reported in the
Literature (regulated by National Agency of Drug and Food Control of the Republic of Indonesia) is
about 5 mg/kg. All the samples showed cadmium in their formulation in the range of 0.1220- 0.22885
mg/Kg (table 2). So trace amount of cadmium in all tested lipsticks is safe.
Conclusion
Four samples of lipsticks were collected from local markets in Padang, were evaluated for heavy
metals(Cd and Pb) levels by flame atomic absorption spectrophotometer. Cadmium concentration
was lower than safe limit. The lead content of the samples were not detected. Although content of
heavy metals in the tested lipsticks was reasonable, but the continious use of lipsticks can increase
the absorption of heavy metals into the body by swallowing or through dermal absorption of these
products. Therefore, informing the users of lipsticks specially teenagers, of the harmful
consequences of lip products should be a big concern.
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