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CH1131_BiomolecularEngineering_Tutorial1_Questions
25,26,28Aug2015
1. ItmakessensethatDNA,thegeneticmaterialofmostorganisms,shouldbesequesteredina
specificareaofthecelltoprotectitandpreventitfrompotentialdamage.Whatisan
advantageofmakingthemRNA,theworkingcopyofthegeneticcode,somuchmorelabile
thanDNA?
2. Youdiscoveramutantorganismthathasdifficultytranscribingaparticularimportantgene.
Itappearsthatwhentranscriptionofthisgeneshouldbeoccurring,RNApolymerasehas
difficultydeterminingwheretranscriptionshouldstart.WhatDNAsequenceismostlikelyto
beresponsibleforthisproblem?
3. MostmRNAscodingforproteinhavelongstretchesofadenineribonucleotides[poly(A)]on
the3'endofthemessage.DoesthatmeanthateachgenefromwhichthesemRNAsare
derivedhasacorrespondingstretchofpoly(dT)atthe5'endofthetemplatefromwhichthe
mRNAwasformed?
4. Thebuildingblocksofpolymersarecalled_______.
a. minipolymers
b. monoblocks
c. monomers
d. portions
e. octamers
9. Whichofthefollowingisanucleoside?
a. phosphate+ribose
b. adenine+deoxyribose
c. sugar+nitrogenousbase
d. adenine+ribose+phosphate
e. bandc
10. Whichofthefollowingisanucleotide?
a. phosphate+ribose
b. adenine+deoxyribose
c. sugar+nitrogenousbase
d. adenine+ribose+phosphate
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e. bandc
11. 35.TheDNAbuildingblocksareknownas________.
a. phosphodiesters
b. nucleosides
c. nucleotides
d. phospholipids
e. aminoacids
12. 36.Thebuildingblocksofanucleotideare___________.
a. apentosesugarandaphosphategroup
b. apentosesugarandanitrogenousbase
c. aphosphategroupandanitrogenousbase
d. apentosesugar,aphosphategroupandanitrogenousbase
e. apentosesugar,aphosphategroupandanaminoacid
13. Purinesarecharacterizedby_______;pyrimidinesarecharacterizedby______.
a. tworings;onering
b. onering;tworings
c. tworings;threerings
d. tworings;tworings
e. tworings;onechain
14. ThetwostrandsoftheDNAdoublehelixarecharacterizedasbeing________.
a. antiparallel
b. complementarytoeachother
c. identicaltoeachother
d. parallel
e. aandb
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15. WhatgivesaDNAmoleculeitsnegativecharge?
a. deoxyribose
b. ribose
c. phosphategroups
d. chlorineion
e. adenine
16. ThetwochainsoftheDNAdoublehelixareheldtogetherby______.
a. hydrophobicinteractions
b. hydrophilicinteractions
c. vanderWaalsinteractions
d. Hbonds
e. aandc
17. WhatisanadvantageofmakingmRNAcopiesofgenes?
a. ThegeneticinformationcanbestoredrelativelysafelyinthenucleuswhilemRNAisa
workingcopy..
b. mRNAissmallerthanDNAandeasiertomoveoutintothecytoplasmwhereproteinsare
made.
c. MakingmultiplecopiesofmRNAallowsanamplificationofsyntheticoutputoverwhat
couldbemadefromonegeneinDNA.
d. mRNAshaveshorthalflivesallowingacelltochangeitsresponsestotheenvironment
efficiently.
e. alloftheabove
18. Ribosomesaremadeof_______.
a. proteins
b. lipids
c. tRNA
d. rRNA
e. aandd
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19. ThesiteonDNAtowhichRNApolymerasesbindbeforeinitiatingtranscriptioniscalledthe
______.
a. terminator
b. operator
c. promoter
d. enhancer
e. silencer
20. ProteinsthathelpRNApolymeraserecognizepromotersarecalled__________.
a. transcriptionfactors
b. translationfactors
c. elongationfactors
d. proteases
e. aandc
21. Eukaryoteshavearequirementforalargevarietyofaccessoryproteinstohelpthemcarry
outtheprocessoftranscription.Whataretheseproteinscalled?
a. transcriptionals
b. elastins
c. transcriptionfactors
d. elongationfactors
e. transcriptionpolypeptides
22. Whatisthefunctionofthepoly(A)tailonmostmRNAs?
a. tohastendegradationofthemRNA
b. topreventprematuredegradationofthemRNAbyexonucleases
c. topreventprematuredegradationofthemRNAbyendonucleases
d. topreventviralinfections
e. todecreasethestabilityofmRNA
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23. Youhaveattachedsyntheticpoly(T)toachromatographycolumn.Ifyoupassamixtureof
cellularRNAsthroughthecolumn,whattypeofRNAwillbeselectivelyretainedbythe
columnandofwhattypeofchromatographyisthistechniqueanexample?
a. rRNAs,affinitychromatography
b. mRNAs,affinitychromatography
c. mRNAs,ionexchangechromatography
d. mRNAs,gelfiltration
e. rRNAs,affinitychromatography