Professional Documents
Culture Documents
JournalofAccordIntegrativeMedicine
Copyright2008Allrightsreserved.Nopartofthispublicationmaybeusedorreproduced,storedina
retrievalsystem,ortransmittedinanyformsorbyanymeans,electronic,mechanical,photocopying,
recordingorotherwise,withoutwrittenpermission.
Nov.2008,Volume4,Number4
ISSN19324642
ImpactofanexternalenergyonStaphylococcus
epidermis[ATCC13518]inrelationtoantibiotic
susceptibilityandbiochemicalreactionsAn
experimentalstudy
Nov.2008,Volume4,Number4:230235
MahendrakumarTrivedi(B.E.Founder),ShrikantPatil(B.D.S.Associate)*
SocietyforDivineLife,A14,KanwalApartment,FourBungalows,Andheri(W.),
Mumbai
PinCode400058,MaharashtraINDIA
*Correspondingauthor
ContactNumbers:
MahendrakumarTrivedi:+919324871979
ShrikantPatil:+919869118769
Emailaddresses:
MahendrakumarTrivedi:secretary@divinelife.us
ShrikantPatil:thelifeenergy@gmail.com
Categoryofsubmission:InVitroStudy
Institutioninwhichtheworkwasperformed:
MicrobiologyLaboratoryofP.D.HindujaNationalHospital&MedicalResearch
Centre,Mumbai,IndiaaccreditedbyTheCollegeofAmericanPathologists
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 1/10
5/14/2015 JournalofAccordIntegrativeMedicine
KeywordsAntibioticresistance,BiochemicalReactions,Lyophilization,
Polymorphism,Energy,Model
Abstract
Purpose
Whilespiritualandmentalenergiesareknowntoman,theirimpacthasneverbeen
scientificallymeasurableinthematerialworldandtheyremainoutsidethedomainof
science.ThepresentexperimentonStaphylococcusepidermis[ATCC13518],
validatetheeffectsofsuchenergytransmittedthroughaperson,Mr.Mahendrakumar
Trivedi,whichhasproducedanimpactmeasurableinscientificallyrigorousmanner.
Methods
Staphylococcusepidermisstrainsinrevivedandlyophilizedstateweresubjectedto
spiritualenergytransmittedthroughthoughtinterventionand/orphysicaltouchofMr.
Triveditothesealedtubescontainingstrain,theprocesstakingabout3minutesand
wereanalyzedwithin10daysafterincubation.Alltestswereperformedwiththehelp
ofautomationontheMicroscanWalkawaySysteminMicrobiologyLaboratory
accreditedbyTheCollegeofAmericanPathologists.
Results
TheresultsindicatedthatMr.Trivedisenergyhaschanged7of27biochemical
characteristicsofStaphylococcusepidermisalongwithsignificantchangesin
susceptibilitypatternin8of29antibiotics.TheBiotypenumberhaschangedfromthe
originalcontrolstraingivingriseto2differentbiotypesintreatedsampleswhilethe
externalenergy/treatmentgivenwasthesameforalltreatedsamplessuggestiveof
randompolymorphismasanalyzedthroughtheautomatedmachine.
Conclusions
Theseresultscannotbeexplainedbycurrenttheoriesofscience,andindicateapotency
inMr.Trivedisenergy,providingamodelforsciencetobeabletoinvestigatethe
impactofspiritualenergyinarigorousmanner.
Inlyophilizedstate,biochemicalandenzymaticcharacteristicscouldbealtered.
Introduction
Alllivingorganismscontainlargecomplexmoleculeswithinthecells.Someofthese
areproteinsandenzymesincludingDNA/RNA.Thenatureoftheorganismsis
dependentonthebiochemicalreactions,whichcanbecharacterizedandidentified.
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 2/10
5/14/2015 JournalofAccordIntegrativeMedicine
Antibioticresistance,regardlessofantibioticandbacteria,willoccurwithsufficient
timeanddruguse.Widespreadantibioticusecausesselectionpressure:resistantstrains
survivewhilesusceptibleonesareeliminated.Antibioticresistanceisprogressive,
increasingfromlowtointermediatetohighlevels[1].Bacterialstrainsarealso
characterizedbytheirrelativeresistancestoarangeofantibiotics.However,sucha
changedoesnottakeplacespontaneouslyinastrainovera10dayperiodofnormal
incubationwithoutcontinuousexposuretoanydrugs.
InthispaperwereporttheimpactofspiritualenergyonStaphylococcusepidermis,in
revivedandlyophilizedstate,withrespecttoitsantibioticsusceptibilitypatternalong
withbiochemicalpropertiesanalyzedwithinaperiodof10days.Thesaidenergywas
transmittedthroughthoughtinterventionofanindividual,Mr.MahendrakumarTrivedi
whohasbeeninteractingwithalargenumberofpeopleasahealeroverthelastdecade.
Asresponsesbyhumanscanbeaccountedforbytheplaceboeffect,theseexperiments
onlowerorganismsweredesignedinordertodirectlytesttheimpactthroughscientific
studiestoruleouttheplaceboeffect.
Itiswidelyacceptedthatlyophilizationisthemethodmostcommonlyusedtostoreand
transportmicrobialculturesaschangeinthebiochemicalandenzymaticcharacteristics
ofanorganismcannotbecarriedoutinthisstate.
MaterialandMethods
TwostrainsofStaphylococcusepidermis[ATCC13518]wereprocuredfrom
MicroBioLogicsinsealedpacksbearingthesameATCCnumberandstoredaccording
totherecommendedstorageprotocolsuntilneededforexperiments.Thestudywas
groupedasperthefollowing.
GroupI:
OneofthetwosealedpacketswashandedovertoHindujaMicrobiologyLabandwas
revivedbythemintwoseparatetubes,ofwhichonewasthecontrol.Thecontroltube
wasanalyzedforidentification,antibioticsusceptibilityandbiochemicalreactionsas
perthestandardprotocolsofsampleprocessinginthemicrobiologylab.
Thesecondtube,(ATCCA)havingviablebacterialculture,washandedoverto
Mr.Trivedifortreatment,aftersealingbyparafilm.Itwasassessedonthe5thand10th
daysaftertreatment.
Treatment:
Mr.Trivediheldthistubeinhishandunderambientconditionsforbetween0.5to3
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 3/10
5/14/2015 JournalofAccordIntegrativeMedicine
minuteswhiletreatingitthroughhisthoughtinterventionprocessbycommunicating
andinstructingtheexperimentalobjectwithinthetubeinordertoundergothechange.
Thetubewasreturnedtothelabinthesealedconditionitself.
GroupII:
ThesecondsealedpacketofStaphylococcusepidermis[ATCC13518](ATCCB)
wastreatedbyMr.Trivedidirectlyinthesealedlyophilizedstate,usingthesame
treatmentprocessasabove.Thesealedtubeswerebrokenandthestrainswererevived
andanalyzedonthe10thdayforidentification,susceptibilitytestingandbiochemical
reactions.AlltestswereperformedwiththehelpofautomationontheMicroscan
WalkawaySystem(DadeBehringSiemens)usingPBPC20panels.Antimicrobial
susceptibilitywasdeterminedusingtheMinimumInhibitoryConcentration(MIC)
methodasperthelatestCLSIguidelines.
MicroScan
Afterinoculationandrehydrationwithastandardizedsuspensionoforganismand
incubationat350cfor16hrs,theminimuminhibitoryconcentration(MIC)orthe
qualitativesusceptibility(susceptible,intermediateorresistant)wasdeterminedby
observingthelowestantimicrobialconcentrationshowinginhibitionofgrowth.The
resultsofsusceptibilitytestingwereexpressedinmillimetersofgrowthinhibitionwith
disktestingandinmcg/mlinMICtesting.
Qualitycontrol:
Theacceptabilityoftheidentificationmediaandantimicrobialagentswaschecked
priortothestudybyATCCcontrolorganisms,SAureusATCC29213,&EColiATCC
25922.
Panelused:PBPC20
ListofAntibioticstested:
Amoxycillin/kclavulanicacid,Ampicillin/Sulbactam,Ampicillin,
Azithromycin,Cafazolin,Cefepime,Cefotaxime,Ceftriaxone,
Cephalothin,Chloramphenicol,Ciprofloxacin,Clindamycin,
Erythromycin,Gatifloxacin,Gentamicin,Imipenem,Levofloxacin,Linezolid,
Moxifloxacin,Nitrofurantoin,Norfloxacin,Ofloxacin,Oxacillin,Penicillin,Rifampin,
Synercid,Tetracycline,Trimethoprim/SulfaandVancomycin.
FollowingBiochemicaltestswereperformed:
Urea,Lactose,Arginine,CrystalViolet,Novobiocin,IndoxylPhosphatase,Voges
Proskauer,Nitrate,Pyruvate,Glycosidases,Arabinose,Raffinose,Sorbitol,RBS,PGR,
MS,OPT,PHO,BE,PYR,MAN,TRE,MNS,BAC,HEM,INUandNACL
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 4/10
5/14/2015 JournalofAccordIntegrativeMedicine
Setting:
MicrobiologyLaboratoryofP.D.HindujaNationalHospital&MedicalResearch
Centre,Mumbai,IndiaaccreditedbyTheCollegeofAmericanPathologists
Results:
Inthispaper,onlythechanges/variationsinantibioticsusceptibilityandbiochemical
reactionsthatwereobservedbeforethetreatment(control)andafterthetreatment
(treated)havebeenreportedinatabularformattachedherewith.
Theresultsoftheantibioticsusceptibilitytests,identificationtestsandbiochemical
testscanbeseeninTable1.
DetailsofMICvalueshavebeenpresentedinTable2.
AntibioticSusceptibility:
Fromtables1&2,itcanbeseenthatchangeshaveoccurredinboththerevivedandthe
lyophilizedformsaftertreatment.
GroupI:
Fortwooftheeightantibioticsshownhere,ChloramphenicolandGentamicin,theMIC
valuesinthecontrolstrainhavedecreasedinthetreatedsamplebythe5thday,
changingthesusceptibilityfromresistantinthecontrolsampletoasensitivestrain.
Andthesamesamplefurtheragainbecameresistantbythe10thdayattainingincrease
inMICvalues.
GroupII:
InGroupIIsamplestoo,althoughtreateddirectlyinthelyophilizedform,achange
maybenotedinthesusceptibilitytoAzithromycin,whoseMICvaluesdecreasedafter
treatmentfrom>4inthecontrolto<=2inthetreatedsample,changingthe
susceptibilityfromresistantinthecontroltosensitiveinthetreatedsamplebyDay10.
Forfiveoftheeightantibioticsshownhere,Ciprofloxacin,Gatifloxacin,Levofloxacin,
OfloxacinandMoxifloxacin,theMICvaluesinthecontrolstrainhaveincreasedin
thetreatedsample,changingthesusceptibilityfromsensitiveinthecontrolsampleto
aresistantstrainby10thday.
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 5/10
5/14/2015 JournalofAccordIntegrativeMedicine
OrganismIdentification:
Biotypenumbersofparticularorganismswerearrivedatafterinterpretingtheresultsof
thebiochemicaltests.TheBiotypenumbersthenledtotheparticularOrganism
Identification.
Surprisingly,thebiotypeshavechangedintwoofthreetreatedsamplescomparedto
controlanddidnotmatchwitheachother.InGroupIIsample,whichwastreatedin
lyophilizedformitself,thespeciesitselfhasbeenidentifiedtobedifferentbyDay10.
BiochemicalReactions:
GroupI:
InGroupI,forRaffinoseandArginine,thereactionwaspositiveinthecontrolsample
andhadchangedtonegativeby5thDayinthetreatedsamplebutreturnedtopositive
byDay10.RestofthebiochemicalreactionsdidnotshowanychangesinGroupI
samples.
GroupII
Forsevenofthebiochemicaltestschangesmaybenoted,whichwastreateddirectlyin
thelyophilizedform.ThereactionwithRaffinose,Arginine,VogesProskauer,BE,
PYRandUreahadchangedfromapositiveresultinthecontroltonegativebythetime
itwastestedonthe10thday.Arabinosehasalsochangedfromnegativetopositive,
althoughitremainedunaffectedintreatedsamplesofGroupI.
Discussion:
Whilemisidentificationsanderrorsinassessmentoftheabovecharacteristicsare
knowntohaveoccurredinsuchtests,theexistenceofchangesinsomanyproperties
andtestscannotbedismissedasamachineerror.Thefindingsofalterationsin
antibioticsusceptibilitypatternsaswellasinseveralbiochemicalreactionsasaresult
ofthetreatmentappliedareunexpectedandunprecedentedbasedontheliteratureso
far,andcurrentlyunexplainedbyscience.Thebiotypesoftheorganismshavealso
changedaftertreatmentbyMr.Trivedi.Thisappearstoindicatethepresenceofan
energytowhichtheorganismswereexposed.Andmostimportantisthattheparticular
microbereactedtothisenergy,whichhasneverbeenreportedyet.
Inseparatestudies,theeffectofMr.TrivedisenergyonbacterialstrainsofATCC
throughDNAfingerprintinghasindicatedDNApolymorphismrangingfrom4%to
79%[2](unpublished).Further,16SrDNAtestsshowachangeinthebacterial
species.[3].Suchobservationsareindicativeofalterationsingenotype.Thesimilarity
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 6/10
5/14/2015 JournalofAccordIntegrativeMedicine
ofeffectofMr.Trivedisuniqueenergyonbiochemicalchangesandsusceptibility
patternsofStaphylococcusepidermis[ATCC13518]andotherbacteriareferenced
above[2,3]leadustopostulatethatStaphylococcusepidermis[ATCC13518]hasalso
gonethroughagenotypechange.ThusweexpecttoseeDNApolymorphisminthese
strains,whichmayberesponsibletocausesuchchangesinantibioticsusceptibilityas
wellasinbiochemicalreactions.
Theresultsobtainedinthepresentexperimentindicatetheexistenceofanenergythat
isstillnotknowntocontemporaryphysicalandlifesciencesandwhichcaneffectively
alterthecharacteristicsofthemicroorganismssuchasthebacteriastudiedinthiswork.
Itisnotpossibletofindanexplanationofsuchobservationsbasedonthecurrent
scientificknowledge.Theseresultsmaygiverisetobetterinsightintothepathways
thatleadtomutationandmayalsohelptounderstandthepossiblebiochemical
pathwaysthattakeplaceinthelifecycleoftheparticularmicrobe.
Whilesciencehassofarremainedunabletoenterintoquestionsregarding
consciousness/Spiritualismandorderedforcesorenergieswhichcaninterconnect
materialsonaplaneofhigherinformation,theresultsofthecurrentexperimentsprove
theexistenceofadirectmodelforinvestigationintotheimpactofaspiritualenergyin
arigorousmanner.
Conclusions:
1Mr.Trivedisenergyhasalteredthebiochemicalreactionsalongwiththeantibiotic
susceptibilityofStaphylococcusepidermisonlywithintheperiodof10days.
2.Thechangesoccurredinsevenbiochemicalreactionsinthelyophilizedstateare
impossibleasperthepresentknowledgeandwouldbeconsideredmiraculous.
3.Lyophilizationisthemethodmostcommonlyusedforthepreservationof
microorganisms,asitdoesnotaltertheChemicalandenzymaticcharacteristicsofthe
microbes.Oncontrary,thepresentedresultshaveobservedthechangesinfew
characteristicsofS.epidermisinthelyophilizedstateitselfindicatingtheneedfor
superiormethodforstorageofparticularmicroorganism.
4.Theseresultscannotbeexplainedbycurrenttheoriesofscience,andindicatea
potencyinMr.Trivedisenergy,providingamodelforsciencetobeabletoinvestigate
theimpactofspiritualenergyinarigorousmanner.
5.Inlyophilizedstate,biochemicalandenzymaticcharacteristicscouldbealtered.
Acknowledgements
TheauthorswouldliketoacknowledgeMs.NandiniAltekarforhelpingusinwriting
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 7/10
5/14/2015 JournalofAccordIntegrativeMedicine
thepaper.
References:
ChangingAntibioticSusceptibilityPatternsataUniversityHospital[SouthMedJ
94(6):619620,2001.2001SouthernMedicalAssociation]
http://www.divinelife.us/Genetics/staphylococcus_aureus.htm
http://www.divinelife.us/Genetics/B_Nocardia_otitidis_Treated_A.htm
Table1:Analysisofbiochemicalreactions,Biotype,OrganismIdentificationNameand
Antibioticsusceptibilitypatternsofallsamples.
GROUPI GROUPII
Nameof Antibiotics& Control Treated Treated Lyophilized
the Biochemical 10thday Treated
bacteria Reactions 5thday Directly,
& 10thday
ATCC
no.
ATCCB
ATCC
ATCC A
A
S.
epidermis
ATCC Azithromycin R R R S
13518
Ciprofloxacin S S S R
Gatifloxacin S S S R
Levofloxacin S S S R
Ofloxacin S S S R
Moxifloxacin S S S R
Chloramphenicol R S R R
Gentamicin R S R R
Biotype 757777 757677 757777 753057
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 8/10
5/14/2015 JournalofAccordIntegrativeMedicine
Organism S. S. S.
epidermis epidermis
Identification epidermis S.sciuri
Name
Raffinose + +
Arginine + +
VogesProskauer + + +
BE + + +
PYR + + +
Urea + + +
Arabinose +
Key:S=susceptible,I=intermediate,R=resistant,+=positive,=negative
Legend:
ControlreferstoATCCstrainsthatwereanalyzedbeforethetreatment.
TreatedreferstoATCC'A'on5th&10thdayafterthetreatment&
ATCC'B'lyophilizedthatweretreateddirectly
Table2:MinimumInhibitoryConcentrationvaluesofantibioticsinmcg/ml.
GROUPI GROUPII
Antibiotics Control Treated Treated Lyophilized
10thday Treated
5thday Directly
ATCCA ATCCB
ATCCA
Azithromycin >4 >4 >4 <=2
Ciprofloxacin <=1 <=1 <=1 >2
Gatifloxacin <=2 <=2 <=2 >4
Levofloxacin <=2 <=2 <=2 >4
Ofloxacin <=2 <=2 <=2 >4
Moxifloxacin <=2 <=2 <=2 >4
Chloramphenicol >16 <=8 >16 >16
Gentamicin >8 <=4 >8 >8
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 9/10
5/14/2015 JournalofAccordIntegrativeMedicine
http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 10/10