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Robert F. Weaver. Molecular Biology. 600 Pages. Fourth Edition. McGraw-Hill International Edition. ISBN 978-
0-07-110216-2.
Prokaryote Genes Are Grouped in Operons
Robert F. Weaver. Molecular Biology. 600 Pages. Fourth Edition. McGraw-Hill International Edition. ISBN 978-
0-07-110216-2.
TRANSCRIPTION INITIATION
Rho- dependent terminators consist of an inverted repeat, which can cause a hairpin
to form in the transcript, but no string of T's. Rho binds to growing transcript, follow
the RNA polymerase, catches the polymerase when it pauses at the hairpin, and
release the transcript from the DNA polymerase complex by unwinding the RNA-
DNA hybrid. This terminate transcription
Robert F. Weaver. Molecular Biology. 600 Pages. Fourth Edition. McGraw-Hill International Edition. ISBN 978-
0-07-110216-2.
SALWA HASSAN TEAMA 2017
The general transcription factors combine with RNA
polymerase form a preinitiation complex that is competent to
initiate transcription as soon as nucleotide are available.
Model of RNA Polymerase II Transcription Initiation Machinery.The machinery depicted here encompasses over 85 polypeptides in ten
(sub) complexes: core RNA polymerase II (RNAPII) consists of 12 subunits; TFIIH, 9 subunits; TFIIE, 2 subunits; TFIIF, 3 subunits; TFIIB,
1 subunit, TFIID, 14 subunits; core SRB/mediator, more than 16 subunits; Swi/Snf complex, 11 subunits; Srb10 kinase complex, 4
http://www.bio.davidson.edu/courses/genomics/2002/james/favoriteyeastproteins.htmsubunits; and SAGA, 13 subunits. This figure
.Comprehensive Yeast Genome Database: provided by Example
ELONGATION
Creation of transcription bubbles
Continuous addition of NTPs, the elongation complex continuous
elongating the RNA.
TBP and TFIIB remains at the promoter. TFIIE and TFIIH are not needed
for elongation and dissociate from the elongation complex.
TERMINATION
The region downstream of the polyadenylation site is essential for
termination.
Cleavage of the nascent transcript at multiple sites downstream of the
polyadenylation sites downstream of polyadenylation site is required for
termination.
The product is immature mRNA Pre mRNA
(Primary transcript)
TRANSLATION
Translation
Ribosomes
tRNA
mRNA template
Amino Acids
Initiation factors
Elongation factors
Termination factors
Aminoacyl tRNA synthetase
Energy source
Three Sites
A site bind to an aminoacyl
tRNA (tRNA bound to an amino
acid).
P site bind a peptidyl tRNA (a
tRNA bound to peptide being
synthesized).
E site binds a free tRNA before
it is exist the ribosome.
SALWA HASSAN TEAMA 2017
Preparatory Steps For Protein Synthesis
Dissociation of the 70s ribosomes into 50s and 30s subunits under the influence of IF1
Binding of IF3 to the 30S subunit, which prevents reassociation between the ribosomal
subunits.
Binding of IF2,IF2 and GTP alongside IF3.
Binding of mRNA and fMet-tRNAfMet to form the 30S initiation complex. These two
components can apparently bind in either order, but IF2 sponsors fMet-tRNA fMet
binding, and IF3 sponsors mRNA binding. In each case, the other initiation factors also
help.
Binding of the 50S subunit, with loss of IFI and IF3
Dissociation of IF2 from the complex, with simultaneous hydrolysis of GTP. The
product is 70 S complex ready to begin elongation.
The initiation codon in prokaryotes is usually AUG, but it can also be GUG, or more
rarely, UUG.
The initiating aminoacy1-tRNAfMet. Is N-formy1-methionine (fMet) is therefore the
first amino acid incorporated into a polypeptide, but it is frequently removed from the
protein during maturation.
The 30S initiation complex is formed from a free 30S ribosomal subunit plus mRNA and
fMet-tRNAfMet.
Binding between the 30S prokaryotic ribosomal subunit and the initiation site of an
mRNA depends on base pairing between a short RNA sequence called the Shine-
Dalgarno sequence just upstream of the initiation codon, and a complementary sequence
at the 3- end of the 16S rRNA. This binding is mediated by IF3, with help from IF1 and
IF2. All three initiation factors have bound to the 30S subunit by this time.
Daniel H. Farkas. DNA Simplified: The Hitchhiker's Guide to DNA. 110 pages. Washington, DC: AACC Press, 1996, ISBN 0-915274-84-1. Available
in paper copy from the publisher
Innis, David H. Gelfand, John J. Sninsky. PCR Applications: Protocols for Functional Genomics: 566 pages. Academic Press; 1 edition (May 17,
1999). ISBN:0123721865. Available in paper copy from the publisher
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter. Molecular Biology of the cell. 1392 pages.Garland
Science; 5 edition (November 16, 2007).ISBN. 9780815341055. Available in paper copy from the publisher
Robert F. Mueller, Ian D. Young. Emery's Elements of Medical Genetics: Publisher:Churchill Livingstone. 1995 ISBN. 044307125X.
Available in paper copy from the publisher.
Robert F. Weaver. Molecular Biology. 600 Pages. Fourth Edition. McGraw-Hill International Edition. ISBN 978-0-07-110216-2.
Available in paper copy from the publisher
William B. Coleman, Gregory J. Tsongalis. Molecular Diagnostics. For the Clinical Laboratorian: 592 pages.Humana Press; 4th Printing. edition
(August 15, 2005). ISBN 1588293564... Available in paper copy from the publisher.