BOD in Star Series

Method Note BOD in

Wastewater M-1013-E 03/05 RevB Wastewater

Calibration and Analysis Key Words Introduction

1. Before calibration and measurement be sure that the probe has polarized for 30 to 50 minutes, the This procedure uses the Orion AUTO-STIR Dissolved Oxygen Probe to measure dissolved oxygen in
air calibration chamber has had at least 10 to 15 minutes to attain proper humidity and air saturated Biochemical wastewater and BOD samples. The Orion 086030MD Probe ts directly into a BOD bottle and uses a
water samples have been properly aerated with oxygen. Oxygen Demand motorized stirrer to properly measure dissolved oxygen.
2. Place the electrode into the calibration chamber and insure that the calibration chamber is wet and has (BOD)
been given proper time to come to equilibrium. Recommended Equipment Cat. No.
Wastewater
3. Press the CALIBRATE key to activate calibration.
AUTO-STIR DO Application Package (includes all items with a Cat. No.) 1010114
4. When the arrow on DO calibration screen stops flashing, press the CALIBRATE or MEASURE key
DO/BOD Probe 1. 3-Star Benchtop DO meter 1113000
to accept the calibration and return to MEASURE mode.
2. AUTO-STIR DO/BOD Probe 086030MD
5. Rinse the electrode thoroughly with Deionized water.
3. BOD bottles (300 mL)
6. Place the electrode in a BOD bottle with a sample.
7. Turn the stirrer on by pressing the button on the probe.
Required Solutions Cat. No
8. Press the MEASURE key on the meter. The mg/L icon will flash as the measurement is being made.
The mg/L icon will become solid and the display value will freeze when a stable reading is achieved. 1. Electrolyte Solution 080514
This value is logged and printed automatically. 2. Deionized water
9. Turn the stirrer off by pressing the button on the probe.
10. Repeat steps 6 through 9 for additional samples. Upon completion of samples, rinse electrode with Calibration Preparation
Deionized water and store the electrode according to instructions in the Electrode Storage section of 1. The 086030MD probe comes with a calibration chamber that also acts as a stand for storage when
this method note. the probe is not in use.
2. Before performing the air calibration unscrew the bottom of the probe stand and remove the
References sponge within.
1. APHA, AWWA and WPCF, Standard Methods for the Examination of Water and Wastewater, 20th 3. Moisten the sponge until it is damp and then squeeze the excess of water from the sponge.
Edition 2000, Jointly edited by Eaton, Andrew D.; Clesceri, Lenore S.; Greenberg, Arnold E.
4. Place the sponge back in the bottom of the chamber and screw the stand back on the
calibration chamber.
5. This will provide a humidified environment for the probe to calibrate in air.
6. The probe can also be calibrated in a BOD bottle that has approximately 1 inch of water in it.

Sample Preparation
Refer to section 5210 B in Standard Methods for the Examination of Water and Wastewater for sample
handling and preparation requirements. (See Item #1 in the Reference section)

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 BOD in
Wastewater

Results Meter Setup Dissolved Oxygen benchtop

Five samples of wastewater from a wastewater treatment facility were measured for DO. The table below For initial meter setup, follow the steps on the Quick Reference Guide that is attached to the meter itself. The
provides data for dissolved oxygen in this sample: Quick Start Guide included with each meter also contains a layout of the meter keypad for reference. The
words in this method in all capital letters such as POWER indicate a key on the meter, and words in quotations
such as Unit indicate information on the meter display.
Prepared for BOD Dissolved Oxygen, mg/L
Wastewater samples 1. Connect the electrode to the meter.
2. Press the POWER key on the meter to turn the meter on.
Sample # 1: 7.91
3. Note that the arrow on the left of the screen indicates the active line. If the bottom line is not active,
Sample # 2: 7.96
press the LINE SELECTION key to change the selected line to the bottom line. The DO measurement
Sample # 3: 8.08 is active.
Sample # 4: 8.38 5. Press the SETUP key to enter DO Setup. Press the LINE SELECTION key to select the middle line.
Sample # 5: 8.14 Press the UP or DOWN arrow keys to select rES mg/L, which is an abbreviation for resolution and
desired result units (mg/L or % sat).
6. Press the LINE SELECTION key to select the bottom line. Press the UP or DOWN arrow keys to select
Mean: 8.09 0.01 with mg/L for rES which is an abbreviation for resolution. Press the LINE SELECTION key to
Standard Deviation: 0.18 accept setting.
%CV: 2.28 7. Press the LINE SELECTION key to select the middle line, and press the DOWN arrow key to select
SAL which is an abbreviation for salinity correction factor type (Auto/Manual).
Electrode Storage 8. Press the LINE SELECTION key to select the bottom line. Press the UP or DOWN arrow keys to select
mAn for salinity correction type. Press the LINE SELECTION key to accept setting.
For short-term storage, the probe should be unplugged from the meter and stored in a humidied environment
9. Press the LINE SELECTION key to select the middle line. Press the UP or DOWN arrow keys to select
such as a calibration chamber or stand. It may also be stored in a BOD bottle partially lled with water.
SALF which is an abbreviation for manual salinity correction factor.
For long-term storage, disconnect the probe from the meter, remove the membrane cap, clean and dry
10. Press the LINE SELECTION key to select the bottom line. Press the DECIMAL/DIGIT key and UP or
the probe.
DOWN arrow keys to select the 0000 for SALF. Press the LINE SELECTION key to accept salinity
factor value (the salinity factor range is 0 to 42 part per thousands).
Equipment Setup
11. Press the LINE SELECTION key to select the middle line, and press the UP or DOWN arrow keys to
Electrode Setup AUTO-STIR DO/BOD Probe select bar to select the barometric pressure compensation type (AUtO/mAn).
1. The 086030MD comes with a membrane already installed and is ready for use after proper polarization of 12. Press the LINE SELECTION key to select the bottom line. Press the UP or DOWN arrow keys to select
the probe. AUtO which is an abbreviation for automatic using the internal Barometer.
2. Once the probe is connected to the meter, the stirring mechanism could be turned on and off by pressing 13. Press the LINE SELECTION key to select the middle line, and press the UP or DOWN arrow keys to
the button on the probe. select CALt to select the DO Calibration type selection.
3. Make sure the probe is inserted into a BOD bottle or sample before pressing the stir button. 14. Press the LINE SELECTION key to select the bottom line. Press the UP or DOWN arrow keys to select
Air for CALt. Press the LINE SELECTION key to accept the setting.
4. Membrane maintenance should be performed when the probe does not provide a proper slope or readings
become unstable. 15. Press the MEASURE key to return to the measurement mode.
5. Refer to the Orion 086030MD AUTO-STIR DO/BOD Probe instruction manual for the maintenance
procedure. If all steps were followed correctly the meter display will show three digits in the top line and ISE: mg/L to
the right of the top line. The meter and electrode are now ready for calibration.

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