Professional Documents
Culture Documents
LABORATORY FILTRATION
Quantitative filter papers are recommended for quantitative analysis. As quantitative analysis requires a high puri-
ty, quantitative papers are washed out with acid.
We offer two grades of quantitative filter papers ashless and ashless hardened. Our ashless grades are made for
100% cotton linters with an ash content of 0,007%. The ashless hardened grades are made of pure cellulose with
an alpha cellulose content of almost 100%. The ashless hardened grades are wetstrengthened.
Ashless papers:
QUALITY BASIS WEIGHT RETENTION FILTRATION SPEED PORE size
g/m2 (Herzberg) ml/min. / sec. MPS
Herzberg DIN 53137 m
00H 80 1 40 160 1 2
006 100 1 80 75 3 4
00A 80 2 125 48 4 5
00K 80 2 200 29 5 6
00M 90 3 450 13 810
00R 90 3 700 11 >10
005 130 3 1000 9 >20
STANDARD SIZES
Circles, in mm
45 47 50 55 70 90 110 125 150 185 240 320
Sheets in mm
470 x 470 470 x 580
QUANTITATIVE PAPERS
LABORATORY FILTRATION
QUALITY FILTRATION PROPERTIES APPLICATION / FIELD OF USE
No. 1 / N wide pores, fast filtering, for coarse, voluminous precipitates like
ashless hardened iron, cobalt and chromium hydroxide,
copper, bismuth, cobalt and iron sulphide,
standard filter for Si-determination in steel
and pig iron analysis.
No. 2 / N medium wide pores, grade most used for quantitative tasks,
medium fast filtering, e.g., filtration of sulphides, lead chromate
ashless hardened and calcium oxalate.
No. 4 / N very narrow pores, slowly special filters for finest precipitates such
filtering, ashless hardened as barium sulphate, meta-stannic acid,
cuprous oxide.
No. 005 very wide pores, very fast for coarse, voluminous precipitates, like
filtering, ashless iron, cobalt and chromium hydroxide,
copper, bismuth, cobalt and iron sulphide,
standard filter for Si-determination in steel
and pig iron analysis.
No. 00R wide pores, fast filtering, ashless. for coarse, voluminous precipitates, like
iron, cobalt and chromium hydroxide,
copper, bismuth, cobalt and iron sulphide,
standard filter for Si-determination in steel
and pig iron analysis.
No. 00M medium wide pores, medium to fast grade most used for quantitative tasks,
filtering, ashless. e.g., filtration of sulphides, lead chromate
and calcium oxalate.
No. 00K medium wide pores, medium to slow grade most used for quantitative tasks,
filtering, ashless. e.g., filtration of sulphides, lead chromate
and calcium oxalate.
No. 00A medium wide pores, medium to slow particularly indicated for fine precipitates
filtering, ashless. such as coarser form ob barium sulphide.
No. 006 narrow pores, slow filtering, ashless special filters for finest precipitates such
as barium sulphate, meta-stannic acid,
curpous oxide.
No. 00H very narrow pores, very slow filtering, special filters for finest precipitates such
ashless as barium sulphate, meta-stannic acid,
curpous oxide. 15
LABORATORY FILTER PAPERS
LABORATORY FILTRATION
These extremely pure filter papers grade 120H to 5 are produced of pure cotton pulp. Grade 1120 are produced
with an alpha cellulose content of almost 100%. Types 1114 correspond in their filtration properties to our
quantitative papers No. 14. The ash content for the grade 120H to 5 amounts to about 0,06%. For Grade 1120
the ash content amounts to <0,1%.
TYPICAL VALUES
QUALITY BASIS WEIGHT SEPARATION CAPACITY FILTRATION SPEED PORE size
g/m2 mA (micro-Ampre) Herzberg sec. / MPS
ml/min. DIN 53137 m
11 85 18 900 10 12
12 85 10 400 25 7
13 85 10 250 60 5
14 85 9 80 150 3
15 70 10 350 25 8
20 120 8 500 11 12
STANDARD SIZES
Circles, in mm
45 47 55 70 90 110 125 150 185 240 270 320 385 450 500
Sheets in mm
480 x 480 480 x 580 580 x 580
QUALITATIVE PAPERS
LABORATORY FILTRATION
Quality Filtration properties Application / Field of use
No. 11 wide pores, fast filtering, for coarse and voluminous precipitates like
smoothened, wetstrengthened hydroxides and sulphides as well as Si-determination
during steel and pig iron analysis.
No. 12 medium to wide pores, medium mostly used for qualitative filter for sulphides, lead
fast filtering, smoothened, chromate and calcium oxalate. Recommended for
wetstrengthened brewery laboratories as it is approved for beer
and malt analysis acc. to EBC regulations.
No. 13 narrow pores, medium fast to suitable for quick filtration of fine precipitates such
slowly filtering, smoothened, as magnesium ammonium phosphate,
wetstrengthened zinc sulphide etc.
No. 14 very narrow pores, slowly special filters for extremely difficult and finely grained
filtering, smoothened, filtration such as cold precipitated barium sulphate,
wetstrengthened metastannic acid, cuprous oxide.
No. 15 medium wide pores, thin, medium thin filters paper for general analytical purposes,
fast filtering, smoothened, slightly filtration of water specimen and for the clarification
wetstrengthened of tinctures, spirits and salt solutions.
No. 20 medium wide pores, thick, medium strong, tough paper, particularly suitable for
fast filtering, high absorption, folded filters. Its high absorption capacity makes
slightly wetstrengthened it an appropriatecarrier for specimen.
No. 5 very wide pores, very fast for coarse, voluminous precipitates, like
filtering, smoothened hydroxides and sulphides as well as
Si-determination during steel and pig iron analysis.
No. 3 wide pores, fast filtering, for coarse, voluminous precipitates, like
smoothened hydroxides and sulphides as well as
Si-determination during steel and pig iron analysis.
No. 150 medium wide pores, medium to grade most used for qualitative tasks for sulphides,
fast filtering, smoothened lead chromate and calcium oxalate.
No.1F medium wide pores, medium fast grade most used for qualitative tasks for sulphides,
filtering, smoothened lead chromate and calcium oxalate.
No. 110 medium wide pores, medium to slow particularly indicated for fine precipitates
filtering, smoothened such as coarser form of barium sulphate.
No. 106 narrow pores, slow filtering, special filters for extremely difficult and finely
smoothened grained filtration such as cold precipitated barium
sulphate, metastannic acid cuprous oxide.
No. 120H very narrow pores, very slowly special filters for extremely difficult and finely
filtering smoothened grained filtration such as cold precipitated barium
sulphate, metastannic acid cuprous oxide.
17
LABORATORY FILTER PAPERS
LABORATORY FILTRATION
Quality Basis Retention Filtration speed Absorption Ash Finish Pore size
weight Herzberg Herzberg DIN 53137 Height Content MPS
g/m2 ml/min. sec. mm % m
1001 90 slow 1 50 112 40 0.1 wetstrengthened 2
1002 90 middle 2 250 28 60 0.1 wetstrengthened 6
1003 90 fast 3 750 10 80 0.1 wetstrengthened >10
STANDARD SIZES
Circles, in mm
45 47 55 70 90 110 125 150 185 240 270 320 450 500
Sheets in mm
480 x 580 480 x 480 580 x 580
These filter papers can also be supplied as folded filter papers.
18
LABORATORY FILTER PAPERS
LABORATORY FILTRATION
FOLDED FILTER PAPERS
With folded filter papers the whole of the surface area is available for filtering, thus increasing the loading capacity.
The flow rate is also improved because less of the filter paper is in contact with the inside of the funnel time is
saved in preparation. We offer folded filter papers for qualitative and for general purpose filters.
STANDARD SIZES
Circles, in mm
45 47 50 55 70 90 110 125 150 185 240 320
19
LABORATORY FILTER PAPERS
LABORATORY FILTRATION
STANDARD SIZES
Folded filters, in mm
125 150 185 240 320
20
GUIDE TO FILTER PAPERS
LABORATORY FILTRATION
is the filtrate to be analysed gravimetrically?
yes yes no
Strong acidic or alkaline
suspension?
Strong acidic or alkaline Filter to be used for general
suspension? purpose?
no yes
no yes yes no
Highly wet-
Highly wet- particle
strengthened yes Technical
strengthened yes retention?
paper? filter papers
paper? absorption?
see pages 7891
and
no Filter papers for
protection?
no special applica-
tions see pages
Precipitate scraped 3136
off or sprayed off yes
no
for analytics?
no yes Please start
at the
left side
005 5 25 m
1/N 11 gel-type 37/N
00R 3 37/N
33/N + 34/N
00M 150 12 m 1003
2/N 12 medium fine
crystalline
00K 1F 7 m 53 + 56
15 1002
00A 110 fine
crystalline 20
3N 13 4 m 75 + 54
006 106 very fine 81/N
4N 14 crystalline
2 m 1001
00H 120 H collodial 95/5
black black ring: ashless fast filtering, red red ring: ashless slowly filtering,
wide pores narrow pores
white white ring: ashless, medium fast blue blue ring: ashless, very slow filtering,
filtering, medium wide pores fine pores
21
CHROMATOGRAPHY AND BLOTTING PAPERS/MEMBRANES
Made of pure cotton linters. Produced with an -cellulose content of Quality Format Package
almost 100%. Graduated by basis weight and absorption capacity. mm
206 580 x 600 100 sheets
P In order to obtain reproducible measurements, work should always 207 580 x 600 100 sheets
be carried our in the direction of the fibres. 208 580 x 600 100 sheets
P Therefore the capillar rise is always sligthly higher in this direction 211 300 x 600 100 sheets
than opposite. 460 x 570
P A drop of water on a square will indicate the fibre direction. 580 x 600
P The bigger elliptical diam. of the spot is the indication of the fibre
direction. Other sizes upon request.
Standard Sizes
Type strips in mm
208 150 x 20
Type segments-diameter in mm
208 145 265 300
22
CHROMATOGRAPHY AND BLOTTING PAPERS/MEMBRANES
BLOTTING PAPER
LABORATORY FILTRATION
Made of pure cotton fibres. Used pre- Application Quality
ferably by the pulp and paper industry
Slot and Dot Blot process 1621
for water absorption tests according
to COBB. First class high grade, non- Capillary transfer 1626
yellowing, dimensionally stable. Tankblot 1626,1621
Also suitable and applicable in other Semi Dry blotting 1600
sectors, where absorption and thick- Vacuum blotting 1600, 1621
ness are significant characteristics
and features.
Ordering Information:
BLOTTING MEMBRANES
LABORATORY FILTRATION
NITROCELLULOSE has a very favourable combination of high protein binding and low background. If you also
add its ease of wetting you can easily understand its popularity in blotting techniques.
Our 0.2 m membrane is the standard material for protein blotting. The 0.45 m membrane is commonly used for
nucleic transfers and colony/plaque transfers.
For multiple reprobing of nucleic acid blots SUPPORTED NITROCELLULOSE (NC-Support) is the best choice as
standard nitrocellulose has a limited physical strength. The polyester support does not have any effect on the
wetting and binding charateristics. NC-Support is also preferred for repeated colony transfers and UV-crosslinking
of DNA.
The POLYAMIDE MEMBRANES are normally used for transfer and immobilization of nucleic acids.
Art. No. 701 156 701 183, 704 100 704 123
Thermal stability Max temperature, 121C for all membranes when wet, 80C for NC-Support and Poly-
amide when dry
24
INDICATOR PRODUCTS
Lyphan-Indicator papers
LABORATORY FILTRATION
without colour scale
Size: 9 x 65 mm
Typical applications
General tests on acid or alkalic reactions. Test
on neutralisation or if already crossing from
acidic to alkalic phase or opposite has been
effected. Litmuspaper is well known as in-
dicator paper.
25
INDICATOR PAPERS
LYPHAN STRIPS/ROLLS
LABORATORY FILTRATION
26
INDICATOR PAPERS
LYPHAN-Strips Especially suitable for testing in turbid and stained aqueous solutions.
LABORATORY FILTRATION
Sensitivity down to values of 0.05 pH.
Strips 0.2 pH Interval
L 653-8 pH 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2
L 656-8 pH 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4
L 662-8 pH 3.9 4.1 4.3 4.5 4.7 4.9 5.1 5.3
L 665-8 pH 5.2 5.4 5.6 5.8 6.0 6.2 6.4 6.6
L 666-8 pH 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4
L 667-8 pH 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0
L 668-8 pH 5.6 5.8 6.0 6.2 6.4 6.6 6.8 7.0
L 669-8 pH 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0
L 671-8 pH 7.3 7.5 7.7 7.9 8.1 8.3 8.5 8.7
L 676-8 pH 8.6 8.8 9.0 9.2 9.4 9.6 9.8 10.0
Strips 0.3 pH Interval
L 650 pH 0.4 0.5 0.7 0.9 1.1 1.4
L 651 pH 1.0 1.3 1.6 1.9 2.2 2.5 2.8
L 652 pH 1.6 1.9 2.2 2.5 2.8 3.1 3.4 3.7
L 656 pH 2.6 2.9 3.2 3.5 3.8 4.1
L 662 pH 3.9 4.2 4.5 4.8 5.1 5.4
L 664 pH 4.3 4.6 4.9 5.2 5.5 5.8 6.1
L 665 pH 5.2 5.5 5.8 6.1 6.4 6.7
L 668 pH 6.0 6.3 6.6 6.9 7.2 7.5
L 669 pH 6.6 6.9 7.2 7.5 7.8 8.1
L 671 pH 7.5 7.8 8.1 8.4 8.7
L 674 pH 8.2 8.5 8.8 9.1 9.4 9.7
L 677 pH 8.8 9.1 9.4 9.7 10.0
L 680 pH 9.4 9.6 9.8 10.0 10.3
L 683 pH 10.1 10.4 10.7 11.0 11.3
L 686 pH 10.4 10.7 11.0 11.3 11.6
Strips 1.0/0.5 pH Interval
L 695 pH 9.0 10.0 11.0 12.0 13.0 14.0
L 698 pH 10.5 11.0 11.5 12.0 12.5 13.0
L 699 pH 11.5 12.0 12.5 13.0 14.0
Strips Extended range 0.3 pH interval
L 658 pH 2.6 2.9 3.2 3.5 3.8 4.1 4.4 4.7
L 663 pH 3.9 4.2 4.5 4.8 5.1 5.4 5.7 6.0
L 670 pH 6.0 6.3 6.6 6.9 7.2 7.5 7.8 8.1
L 672 pH 6.6 6.9 7.2 7.5 7.8 8.1 8.4 8.7
L 701 pH 11.0 11.3 11.6 11.9 12.2 12.5 12.8 13.1
Special papers
Strips Weak buffer
L 625 pH 3.0 3.3 3.6 3.9 4.2 4.5 4.8 5.1
Strips Beer and other fermentation products
L 659 pH 2.4 2.7 3.0 3.2 3.4 3.7 4.0 4.3
27
SEED TESTING AND SOIL ANALYSIS
LABORATORY FILTRATION
Smaller seeds (grasses etc.) Jacobsen (Copenhagen tank) 1700, 1701, 711 (circles)
Top of paper 1700 (wicks/sheets)
Smaller seeds (grasses etc.) Germination Cabinet 1701 (sheets)
Top of paper 1200 (underlayer) 720
Cereals Rolled towel M-towel
Between paper (pair of 1750 and 1755)
Peas Between paper 1760/716
Beet and pelleted seeds Pleated paper 1765/715
HEALTH TESTS
SPECIES METHOD (apparatus) QUALITY
Smaller seeds (grasses etc.) Jacobsen (Copenhagen tank) 1700, 1701 (circles) 711
Top of paper 1700 (wicks/sheets) 710
Cereals Dishes or boxes 1731 (circles) 713
Top of paper
Cerelas Osmosis test 1731 (circles) 713
28
SEED TESTING AND SOIL ANAYSIS
LABORATORY FILTRATION
GERMINATION AND SEED TEST PAPERS
Our germination and seed test papers are wetstrengthened for use in germination tests of seed specimen to
improve seed growth and growth velocity.
Available both in form of white pleated strips for gemination test especially for beet seed acc. to NEEB and in form
of circles. They are of high purity, offering absolutly reproducible result and are internationally recognised by all
leading laboratories.
Other methods acc. ISTA: Grass Seeds on circles, Germination Test in Petri Dishes/Boxes, Vigour test for Maize, Centian
Seeds, differents treatments.
29
LABORATORY FILTER PAPERS
LABORATORY FILTRATION
You are most welcome to contact us in order to get specific information about soil testing and adequate filter pap-
ters. Our grades are used for soil testing in all european countries.
30
SPECIAL PRODUCTS
LABORATORY FILTRATION
CREPED PAPER
is used as surface protection and for the absorption of liquids in
laboratories.
EDOLAP
PE-laminated EDOLAP paper (LIQUID ABSORBING PAPER). The impermeable
polyethylen backing guarantees the protection of all surface covered such as labo-
ratory table, cupboard or tray. Protects against contamination and allows the reco-
very of precious materials. Also suitable for laying out animal cages.
CREPE
Basis weight Capillary rise
Quality g/m2 mm Finish
250/B 60 100 white, wetstrengthened
EDOLAP
Basis weight Thickness
Quality g/m2 mm Finish
358/PE 150 0,23 ultra-absorbent carrier
material, impermeable
PE-lamination
STANDARD SIZES
Quality Reels Sheets in mm
358/PE 460 mm / 50 m 460 x 570
358/PE 920 mm / 50 m
BENCHBLOT
BENCHBLOT (1370) is a polyethylene coated filter paper for protection of laboratory benches or working areas.
Spill of chemical, toxis, infectious, and radioactive materials etc. is quickly soaked up in the filter paper and the
plastic layer prevents contamination of the surface underneath.
BENCHBLOT SOFT (1371) is a multi-ply tissue with a polyethylene backing and to be used when a fast absorp-
tion with a very high capacity is wanted. Benchblot Soft has also a very soft surface which will not delaminate
when wetted.
BENCHBLOT also makes a hard surface softer, reducing breakage of glass etc.
BENCHBLOT presents no problem after use it is safe to burn.
STANDARD SIZES
Quality Rools in m Sheets in cm
1370 50 x 0.5 43 x 43
100 x 0.5 48 x 60 in dispenser box
1371 50 x 0.6 48 x 60
31
LABORATORY FILTER PAPERS
LABORATORY FILTRATION
32
SPECIAL PRODUCTS
LABORATORY FILTRATION
GRADE BASIS WEIGHT FILTERING SURFACE FINISH
2
g/m SPEED SEC.
54 70 17 plain slightly wetstrengthened
56 90 90 embossed wetstrengthened
69/K 160 65 plain activ carbon treated
95/5 150 70 plain slightly wetstrengthened
120 290 plain slightly wetstrengthened
Grade 54 plain, medium wide pores, medium said to be the universal filter for laboratories
fast filtering, slightly wetstrengthened in the chemical and pharmaceutical industry.
Especially indicated for spirits,
tinctures etc.
Grade 56 embossed, medium wide pores, embossed paper for general applications
medium fast filtering, slightly with suitability for oils, essences,
wetstrengthened tinctures, emulsions
Grade 69/K medium fast filtering, with activated activated carbon treated paper for
carbon, smoothened clarification and brightening of dull and
dark urines, for polarimetric sugar
determination
Grade 95/5 plain, tight, slowly paper with highly ennobled kieselguhr.
filtering, slightly wetstrengthened Filter media of highest separation capacity.
Retains the finest turbidities. For clarification
of urine sugar and starch solutions as well as
milk serum.
Grade 120 plain, medium fast filtering, for gelatine and oil filtration. For use in filter
slightly wetstrengthened presses for transformer oil filtration.
STANDARD SIZES
circles and folded filters, diameter in mm
45 55 70 90 110 125 150
185 240 270 320 385 450 500
Sheets in mm
Parchment Cuttings
For weighing syrup-like, half-crystalline substances or beet pie.
Especially suitable for works in sugar laboratories as it can
easily be beaten into pieces.
Available in sheets of 100 x 100 mm
33
SPECIAL PRODUCTS
Silicone impregnated paper, hydrophobic. Separation of aqueous solutions from organic solutions which are not
soluble with water. Organic phase permeability. Retaining of solid and aqueous components. Usable as disposa-
ble separating funnels. No effect on the separating process due to the density of the organic phase.
LABORATORY FILTRATION
AND DIAGNOSTICS
The following list contains indications as to which grades are recommended for special applications. Quite often
an individual solution is necessary. We offer as follows.
QUALITIES
BMA BMB BMC BMD BMF BMG
MGA MGB MGC MGD MGF MGG
Application
Staining of dyed papers P
Solvent filtration P P
Membrane pre-filters P P
Protein filtration P
Radio-immuno test P P
Scintillation counting P P P
Cell harvesters P P P
Carbohydrate analyses P
35
THIMBLES
Cellulose fibres:
LABORATORY FILTRATION
STANDARD SIZES
inner diameter x height in mm
Micro-glass fibres
Filter thimbles of high purity. For temperatures up to 500C. Particularly suitable for aggressive solvents. For
analytical seizing of environmental pollution.
STANDARD SIZES
inner diameter x height in mm
Micro-quartz fibres
Special development for the analysis of air pollution, binder-free. Resist temperatures of up to 950C.
STANDARD SIZES
inner diameter x height in mm
LABORATORY FILTRATION
MUNKTELL and EDEROL offers you a wide range of diffe-
rent syringe filters. In our programme you will find cellulo-
se acetate-regenerated cellulose-PTFE Polyethersulfone
and micro glass fibre syringe filters.
Our 0.2 micron pore size syringe filters allow quicker steri-
lization of up to 100 ml liquid volumes. Filters with larger
pore sizes from 0.45 up to 5 micron are suitable for
clarification of large volumes.
Colour code
In order to identify the pore size we use the following colour for cellulose acetate syringe filters:
PTFE and Regenerated cellulose syringe filters have CLEAR HOUSING. Prefilters of micro glass fibre paper have
WHITE HOUSING.
37
MICRO FILTRATION
For larger pore sizes (1.2, 3.0, 5.0 and 8.0 m) the bubble points are practically nill.
P Flow rates (mean values per cm2 with water and g p = 1 bar (100 kPa):
0.2 m . . . . . . . . . . . 22 l/min 1.2 m . . . . . . . . . . . . . 320 l/min
0.45 m . . . . . . . . . . . 69 l/min 3.0 m . . . . . . . . . . . . . 430 l/min
0.65 m . . . . . . . . . . . 130 l/min 5.0 m . . . . . . . . . . . . . 570 l/min
0.8 m . . . . . . . . . . . 200 l/min 8.0 m . . . . . . . . . . . . . 750 l/min
P Compatibility:
Stable with solutions in range pH 48, resistant to most alcohols, hydrocarbons and oils. See table of com-
patibilities.
P Limits:
maximum temperature 180C.
P Sterilization:
Autoclave (121C or 134C), with y-radiation or
with ethylene oxide.
P Validation:
Correlation of the values of the bubble point
values of membranes of pore size 0.2 m for
checking sterilizing filtration was validated by
the standard BCT test.
(Bacteria Challenge Test).
LABORATORY FILTRATION
Cellulose ester membrane filters are used in a wide variety of analy-
tical and laboratory procedures that require filtration. The cellulose
nitrate membrane filters is hydrophilic and has uniform pore sturctu-
re over a wide range of pore sizes and therefore is recommended for
sample pretreatment, particle testing and removal.
These membranes have a high non-specific adsorption
which makes them suitable for many blotting procedures
and for diagnostic kits. The adsorption decrease with
increasing pore size.
Sterile individually packed membrane filters are standard
for routine microbiological quality control. Filter identifi-
cation and lot number are printed on each individual
envelope. In our production each membrane and envelope
is checked.
All gridded membranes are made of cellulose nitrate, which assures excellent retention and colony growth. The
grid size is 3.1 x 3.1 mm and can be supplied in black, white and green grid. White membranes with black grid
and green membranes with dark green grid is used for colony counts. Gray/black membranes with white grid is
used for detection of yeasts and moulds. White membranes with green grid ist used for E.coli and coliforms.
In our programme you can also find gridded and sterile membranes with a pink-coloured hydrophobic edge for
sterility testing.
We also offer non-sterile gridded membranes in standard packs for particle counting and microscopy.
P Flow rates (average values per cm2 with water and g p = 1 bar (100 kPa):
0.2 m . . . . . . . . . . . 20 ml/min 0.45 m . . . . . . . . . . . . 47 ml/min
P Compatibility:
Stable with aqueous solutions in pH range 312. Resistant to most solvents.
See table of compatibilities.
P Sterilization:
Autoclave (121C or 134C), dry heat 180C, y-radiation (25 KGy) or with ethylene oxide.
P Validation:
Correlation of bubble point values of membranes of 0.2 m pore size in order to check sterilizing filtration was
validated by the standard BCT test
(Bacteria Challenge Test).
40
MICRO FILTRATION
LABORATORY FILTRATION
The PTFE membrane filter is naturally and permanently
hydrophobic. It permit the passage of air and gases and it
does not become blocked with humidity. If it should be
used for filtration of aqueous solutions, it must be pre-
wetted with a solvent before water passage can occur.
PTFE membrane filters are ideal for venting applications,
phase separations, aerosol samplings and gas filtration.
P Bubble point (minimum value for the 0.2 m membrane and average values for the other poroties,
dampened in all cases with isopropanol):
0.2 m . . . . . . . . . . . 1.2 bar 1.2 m . . . . . . . . . . . . . 0.45 bar
0.45 m . . . . . . . . . . . 0.8 bar 5.0 m . . . . . . . . . . . . . 0.1 bar
P Flow rates (average values per cm2 with air and g p = 0.05 bar):
0.2 m . . . . . . . . . . . 0.2 l/min 1.2 m . . . . . . . . . . . . . 1.6 l/min
0.45 m . . . . . . . . . . . 0.3 l/min 5.0 m . . . . . . . . . . . . . 4 l/min
P Compatibility:
Resistant to most solvents. See table of compatibilities.
P Sterilization:
Autoclave (120C or 134C), with ethylene oxide.
P Thickness:
Varies according to pore diameter, between 65 m (0.2 m) and 100 m (5 m).
P Validation:
Correlation of the bubble point values for membranes of 0.2 m pore size in order to check sterilizing filtration
was validated by the standard BCT test.
(Bacteria Challenge Test).
41
MICRO FILTRATION
P Adsorption: 100 g/cm2 with bovine serum for the 0.2 m membranes.
P Flow rates (mean values per cm2 with water and g p = 1 bar (100 kPa):
0.2 m . . . . . . . . . . . 23 ml/min
0.45 m . . . . . . . . . . . 46 ml/min
P Compatibility:
Stable with solution in pH range 314, resistant to some solvents.
See table of compatibilities.
P Sterilization:
Autoclave (121C or 134C), with ethylene oxide.
P Validation:
Correlation of the bubble point values for membranes with pore size of 0.2 m to check sterilizing filtration has
been validated by the standard BCT test.
(Bacteria Challenge Test).
42
MICRO FILTRATION
LABORATORY FILTRATION
In general glass fibre filters have an open structure with a
large available surface area and filter depth. Compared
with a membrane they have a high dirt-loading capacity.
Because of the high dirtloading capacity they are often
used to protect membrane filters, giving an excellent
throughput and absolute retention.
43
MICRO FILTRATION
Colour code
Blue Yellow Green Red Brown
0.2 0.45 0.8 1.2 5.0 micron
LABORATORY FILTRATION
Inside this syringe filter we have a hydrophilic and solvent resistent regenerated cellulose membrane. This syringe
filter is suitable for ultra cleaning of small volumes of samples for HPLC or GC.
We supply these syringe filters in two pore sizes 0.2 and 0.45 micron and in three sizes 4 15 25 mm:
45
MICRO FILTRATION
This is a filter for rapid, simple and reliable ultra cleaning of small volumes.
PTFE is hydrophobic and has an excellent compatibility with aggressive
samples. These units are recommended for HPLC solvent preparation and
degassing to ensure column protection, trouble free handling and consi-
stent test results. These units can be autoclaved. We supply them in two
pore sizes 0.2 and 0.45 micron and in three diameters 4 15 25 mm:
46
MICRO FILTRATION
LABORATORY FILTRATION
Our new membrane is made of a single layer
polyethersulfone. It offers high flow rates, low extrac-
tables and better strength than cellulose-type mem-
branes. It is suitabel for biological, pharmaceutical
and sterilizing filtration.
Colour CODE
0.20 micron Syringe filters dark blue
48
MICRO FILTRATION
ACCESSORIES
LABORATORY FILTRATION
PTFE Holder for solvents Polycarbonate Holder for
and aggressive liquids aqueous solutions
FILTER HOLDERS
We can offer our customers a wide range of filter holders, depending upon the volume and type of the sample.
The filter holders are used for clarification and sterilization of samples. All our filter holders are re-usable.
The polycarbonate filter holder can be used when filtering aqueous samples while the PTFE holder is recommend-
ed when filtering solvents and aggressive liquids.
49
MICRO FILTRATION
ACCESSORIES
LABORATORY FILTRATION
The polycarbonate filter holder can be used when filtering aqueous samples while the stainless steel filter holder is
recommended when filtering solvents and aggressive liquids.
Code FH FH
Art. No. 730 004/730 005 730 006
Application Aqueous samples Solvents/aggressive samples
Material Polycarbonate Stainless steel
Seal Silicone gasket PTFE
Diameter 25 mm 25 mm
Filter area 3 cm2 3 cm2
Connection, inlet Luer lock Luer lock
Connection, outlet Luer slip Luer slip
Max. pressure 7 bar 7 bar
Sterilization by autoclaving 121C by autoclave 121C or 134C.
Dry heat 180C
50
MICRO FILTRATION
ACCESSORIES
LABORATORY FILTRATION
47 and 50 mm FILTER HOLDERS
These filter holders are used for filtering up to 1000 ml. Our 50
mm polycarbonate filter holder is recommended for filtration
of aqueous samples and has an excellent resistance to pres-
sure. It can be connected to a pressure tank or a peristaltic
pump to filter buffer or other aqueous solutions.
When filtering aggressive samples or gases our 47 mm stain-
less steel filter holder is recommended. The inlet side valve is
used for air-venting when the holder is used as an in-line
sampler for liquids, or for run-off of retained water when filte-
ring gases.
PTFE disc
Viton O-ring
Closing ring
Support screen
Top part
Silicone O-ring
Filter support
Silicone O-ring
Support screen
Base part
Underdrain screen
Base part
Hose nipple
Hose nipple
51
MICRO FILTRATION
ACCESSORIES
LABORATORY FILTRATION
All parts of the holder in contact with the liquid are made of chemically inert glass or PTFE. When used, together,
with a hydrophilic RC-membrane, it is ideal for ultra cleaning and degassing solvents and aggressive chemicals.
The hose nipple connector is made of polypropylene.
Code GFH
Art. No. 732 001
Capacity Funnel 250 ml, Suction flask 1000 ml
Compatibility See compatibility guide
Filtration area 12.5 cm2
Flow rates Typical data for water at 90% vacuum
200 ml/min. (0.2 m), 600 ml/min. (0.45 m).
2.2 l/min. (0.8 m)
Filter size 47 or 50 mm
Materials Flask/Funnel: Borosilicate glass
Clamp: Anodized aluminum
Hose nipple: Polypropylene
Seal ring/Seal gasket: Viton
Max. pressure Vacuum only
Sterilization Autoclave (121C or 134C)
Dry Heat (180C)
Remove hose nipple first
ACCESSORIES
LABORATORY FILTRATION
MANIFOLDS
Manifolds are used to support vacuum filtration funnels for
running multiple filter holders of one vacuum line. The
manifolds are made of stainless steel and are available
with 3 and 6 filtration funnels.
Code M
Art. No. 731 001 731 004
Flow rates Water with 90% vacuum: 600 ml/min (0.45 m)
200 ml/min (0.20 m)
Compatibility Resistant to most solvents. See compatibility guide
Filtration area 12,5 cm2
Materials Stainless steel: Manifold, funnel, lids,
plugs, clamps, frits
PTFE: Connector, gasket
Silicone: Sealing, connector-ring
Filter size 47 or 50 mm
Max. pressure Vacuum
Sterilization Autoclave (121C or 134C)
Dry heat (180C) Lid
Silicone seal
Funnel
with clamp
Clamp
ORDERING INFORMATION SEE PAGE 125
Lid
Silicone seal
Funnel
with clamp
Silicone O-ring
SupportSupport
frit frit
PTFE-gasket
Cap
53
MICRO FILTRATION
SPECIFICATIONS FOR
26 mm VENTING FILTER
Code VPT-26
Art. No. 720 200 720 201
Air flow rates Approx. 1.4 l/min at g p = 1.0 bar
Bubble point Min. values with isopropanol, 1.2 bar
Burst pressure 6.0 bar
Filtration area 5.3 cm2
Water wetting penetration pressure 4.0 bar
54
MICRO FILTRATION
LABORATORY FILTRATION
For containers of culture media, small fermenters and other containers needing sterile ventilation we have develo-
ped a 64 mm venting filter with a hydrophobic PTFE membrane. The membrane is reinforced with a polypropylene
gauze.
We offer two different pore sizes 0.2 and 0.45 micron and two different connectors hose nipple inlet/outlet
and 1/8 male NPT. The venting filters are delivered sterile or non-sterile.
Our unique construction allows high air flow at low differential pressures.
55
MICRO FILTRATION
PUMPGARD
LABORATORY FILTRATION
56
MICRO FILTRATION
Micro Filter MS-20 Dimensions Pressure drop VS. flow graph for
LABORATORY FILTRATION
MS-20
Applications
Micro filtration of supply air and as
venting filter in medicine and for Specification
pharmaceuticals. Casing: Polystyrene
Removal of particles and liquids (at Filter medium: Munktell hydrophobic micro-glass fibrepaper
low pressures) from air/gas sup-
plies going to instruments that are Connections: Nipples connected to filter housing through quick-connect coupling.
Tubes with an inside diameter of 4 to 8 mm can be fitted to the
sensitive to impurities. nipples.
Micro Filter MS-40 Dimensions Pressure drop VS. flow graph for MS-40
Applications
Micro filtration of supply air/gas (as
in CO2-incubators and oxygena- Specification
tors) and as venting filter in medi- Casing: Polystyrene
cine and for pharmaceuticals hand-
Filter medium: Munktell hydrophobic micro-glass fibrepaper
ling and production.
Connections: For tubes with an inside diameter of 46,5 mm
Retention: > 99,999 % (0,3 m)
Max. temp.: 80C
Sterilisation: Can be supplied radiation sterilised.
Micro Filter MS-41 Dimensions Pressure drop VS. flow graph for
LABORATORY FILTRATION
MS-41
Applications
Micro filtration of supply air/gas (as
in CO2-incubators and oxygena- Specification
tors) and as a venting filter in medi- Casing: Polystyrene
cine and for pharmaceuticals hand- Filter medium: Munktell hydrophobic micro-glass fibrepaper
ling and production.
Connections: For tubes with an inside diameter 69 mm
Retention: > 99,999 % (0,3 m)
Max. temp.: 80C
Sterilisation: Can be delivered radiation sterilized
Micro Filter MS-61 Dimensions Pressure drop VS. flow graph for MS-61
Applications Specification
Elimination of bacteria, micro- Casing: Polystyrene
organisms and other particles for
Filter medium: Munktell hydrophobic micro-glass fibrepaper
various types of medical work. Fil-
tration to obtain particle-free air or Connections: For tubes with an inside diameter of 1014 mm
gas to measuring devices to get a Retention: > 99,999 % (0,3 m)
reliable function and high quality. Max. temp.: 80C
Sterilisation: Can be delivered radiation sterilised
LABORATORY FILTRATION
Micro Filter MS-40-S
Applications
Micro filtration of flows up to 3040
l/min to get a sterile and particle- Pressure drop VS. flow graph for
free air or gas. Primarily used to MS-40-S
clean exhaust air from suction
equipment.
Specification
Casing: Polystyrene
Filter medium: Munktell hydrophobic micro-glass fibrepaper
Applications
Micro filtration of flows up to 3040
l/min to get a sterile and particle-
free air or gas. Primarily used to
Pressure drop VS. flow graph for
clean exhaust air from suction Suctogard
equipment.
Specification
Casing: Polystyrene
Filter medium: Munktell hydrophobic micro-glass fibrepaper
Micro Filter MS-681 Dimensions Pressure drop VS. flow graph for
LABORATORY FILTRATION
Applications
Micro filtration of air to cultivation
and storage tanks that require Specification
sterile and particle-free air. In medi- Casing: Polystyrene (cartridge)
cine used among others in auto- Filter medium: Munktell hydrophobic micro-glass fibrepaper
claves, sterilizers, incubators and
Holders: Nickel-plated brass, one half with snap lock
ventilators for venting and to get a
Retention: > 99,999 % (0,3 m)
sterile air supply.
Connections: See page 62
Max. temp.: 80C
Micro Filter LP-10 Dimensions Pressure drop VS. flow graph for LP-10
Applications Specification
Supply and exhaust air filters for Casing: Polystyrene
the ventilation of tanks and other
Filter medium: Pleated Munktell hydrophobic micro-glass fibrepaper with spacers
containers where a sterile environ-
ment is required, for instance in Connections: Outside diameter 38 or 80 mm
breweries, pharmaceuticals manu- Retention: > 99,999 % (0,3 m)
facture and the food industry. Also Max. temp.: 80C
used as an absolute filter for par-
ticles from laser operations.
Every filter is tested for retention.
60
MICRO FILTRATION
Micro Filter LP-025 Dimensions Pressure drop VS. flow graph for
LABORATORY FILTRATION
MS-681 with holder combination
Applications
Micro filtration of air and gases to
Specification
give a sterile and particle-free flow
to medical equipment (ventilators, Casing: Polystyrene
sterilizers..) and in pharmaceutical Styrolux (transparent LP-025)
and food applications (autoclaves, Filter medium: Munktell hydrophobic micro-glass fibrepaper
venting filters..) Retention: > 99,999 % (0,3 m)
Connections: Female connection ISO 22 mm
Male connection ISO 22 mm
Max. temp.: 80C
Package: Single packed
Sterilization: Can be supplied radiation sterilised
Micro Filter LP-050 Dimensions Pressure drop VS. flow graph for LP-10
Applications
Micro filtration of air and gases to
give a sterile and particle-free flow Specification
to medical equipment (ventilators,
sterilizers..) and in pharmaceutical Casing: High-impact polystyrene
and food applications (autoclaves, Filter medium: Munktell hydrophobic micro-glass fibrepaper
venting filters..) Retention: > 99,999 % (0,3 m)
Connections: Female connection ISO 22 mm
Male connection ISO 22 mm
Max. temp.: 80C
Package: Single packed
Sterilization: Can be supplied radiation sterilised
62
MICRO FILTRATION
LABORATORY FILTRATION
Our capsule filters are ready to connect and to use
pre-tested, complete filters. They can be rinsed after
use re-autoclaved and re-used if the application
allows. The cellulose acetate membrane is pleated
inside the polypropylene housing. The housing con-
tains two membrane filters. The first membrane acts
as a prefilter to the finer seond membrane.
Code CCA
Art. No. 722 001 722 020
Adsorption See cellulose acetate in the diagramm on page 38
Biological safety All materials pass USP Plastics Test Class VI
Compatibility Chemically compatibile with aqueous solutions of pH 48
and with most alcohols and hydrocarbons.
Cytotoxicity All materials are non-toxic, as determined with L929
cells and MRC-5 cell
Filtration area 0.05 m2, 0.1 m2, 0.2 m2
Integrity test data All capsules are integrity testable. Details on min.
bubble points and max. air diffusion values are
given in the Direciton for use supplied with them.
Materials Cellulose acetate membrane filters, polypropylene
housing and supporting/drainage layers.
Pressure limit Max. g p = 4 bar at 20C, 2 bar at 80C.
Sterilization By autoclaving at 121C, 30 minutes.
PTFE CAPSULES
LABORATORY FILTRATION
High air flow rates of the membrane and large filtration area allow effective sterile venting at low differential pres-
sure.
As PTFE is extremely hydrophobic we guarantee maximum reliability even when filtering moist air.
Each capsule is a ready to use, pre-tested, complete filter. The pleated PTFE membrane ensures maximal filtration
area.
We offer two different connectors and three different sizes
Differental pressure/
Air flow rate of PTFE
capsules
LABORATORY FILTRATION
SCAP is a pressure filtration unit for reliable sterile filtration of
tissue culture solutions. This filter has a large filtration area and
an automatic venting which allows high flow rates. Attached to
a pump this is a filter for 100 ml up to 5 liter of media and
aqueous solutions. It has a polycarbonate housing and an inlet
female luer lock or hose nipple inlet.
SPECIFICATION OF SCAP
65
NUTRI CULT
In order to avoid time-consuming and extensive preparation of culture media you should use NUTRI CULT.
Our NUTRI CULT is an absorbent pad which has been impregnated with a culture medium, then dried and
sterilized in Petri dishes.
They are available with 6 different media and are supplied in sets, which also contains sterile-individually packed
membrane filters. The membrane is of course chosen for optimum colony growth and colony counting.
The shelf life of the NUTRI CULT is 9 to 24 months, depending to type of medium.
ART.-NO. 733 001/002 733 003/004 733 005/006 733 007/008 733 009/010 733 011/012
TYPE ENDO ORANGE SERUM STANDARD TTC TOMATO JUICE WORT BEER
DETECTION E. coli and Acid tolerant Colony count Leconostoc Yeast and Pediococci
OF coliforms microbes Oenos moulds Lactobacilli
MEMBRANE 0.45 micron 0.45 micron 0.45 micron 0.45 micron 0.65 micron 0.45 micron
white with green with green with green with grey with white with
green grid, dark green dark green dark green white grid green grid
47/50 mm grid, 47/50 mm grid, 47/50 mm grid, 47/50 mm 47/50 mm 47/50 mm
PACK 100 100 100 100 100 100
USE 24 hours 23 days 48 hours 46 days 23 days 23 days
37C 2528C 30C 2530C 25C 2528C
Depending on (anaerob, (anaerobic)
tested material Re check after for quick
you have 10 days in order microscopic
variations to detect slow examination
(4872 hours growing micro-
20C) organisms)
APPLICATION Water Beverages Beverages Wine Beer Beer
Food Wine Water Sugar
Beverages Food Wine
Pharma Food Water
REFERENCE Trinkwasser-VO Recommended Trinkwasser-VO Dubois, Bindan According Emeis
1.1.1991 methods for Standard and Lafonla- to DAB 7 modified
Standard Microbiolo- Methods for fourcade (German acc. to
Methods for gical Exami- the Examina- Pharma- Rinck and
the Examina- nation of tion of water cipoeia) Wacker-
tion of Water Foods (1966), and wastewater bauer
and Waste water APHA MEBAK
(ISO 9308-1) EMB
SHELF LIFE 9 12 24 12 24 12
MONTHS
CULTURE MEDIA
LABORATORY FILTRATION
CFU Determination
67
SEED TESTING AND SOIL ANALYSIS
TYPICAL PROPERTIES
QUALITY WEIGHT ABSORPTION ASH DELIVERY
HEIGHT CONTENT FORM
g/m2 mm %
1700 135 65 0.1 circles with hole
wicks and sheets
1701 135 55 0.1 circles and sheets
1731 400 70 0.1 circles and sheets
STANDARD SIZES
Circles, in cm
7.5 8.5 8.8 9.5 13.0 16.0 16.2 17.0
Wicks in cm
1.8 x 32
Sheets in cm
15 x 15 22 x 22 48 x 48 Available in other sizes
TYPICAL PROPERTIES
QUALITY WEIGHT ABSORPTION ASH SIZE
HEIGHT CONTENT
g/m2 mm % cm
1750 90 80 0.1 22 x 40
1755 53 polyethylene coated 19 x 40