GE Healthcare

KTATMprime
KTATMprime Plus

2.9mm i.d.
3/16" o.d. clear teflon

2.9mm i.d.
3/16" o.d. clear teflon

2.9mm i.d.
3/16" o.d. clear teflon

1.6 mm i.d gradient switch valve to pump

1/8" o.d. teflon clear

0.75 mm i.d From system pump to fraction collector.

1/16" o.d. PEEK Green

1.0 mm i.d Waste tubing (2 pieces)

1/16" o.d. tPEEK Brown


! ( WARNING! )
WARNING

! ( CAUTION! )
CAUTION

( NOTE )
NOTE

GE Healthcare Life Sciences

-
8 2401
200336

( Trademarks )
KTASuperloop,HiTrap,HiPrep,HiLoad,RESOURCE GSTraps GE Healthcare

Amersham GE Healthcare (Drop Design) GE Healthcare

(Term and Conditions of Sale)

GE Healthcare
www.gehealthcare.com/lifesciences

(Addresses)
800 810 9118

lifesciences@ge.com

010 5806 8888 69689

010 6787 1162
100176
1
2


021 5257 4650 67337

021 5208 1282
200336
8 24

020 8363 3828 67961

020 8363 4210
510060
33 1212

852 2100 6314

852 2100 6338
8 12

GE Healthcare - All rights reserved

1 (Introduction) ................................................................................................................... 5
1.1 (General) ................................................................................................................. 5
1.2 (Description) ........................................................................................................... 6
1.3 (Safety) ................................................................................................................... 7
2 (Unpacking and installation) ................................................................................. 9
2.1 (General) .................................................................................................................. 9
2.2 (Pre-requisites).............................................................................................. 10
2.3 KTATMprime (Unpacking KTAprime)........................................ 10
2.4 (Connecting mains cable) .......................................................................... 12
2.5 (Connecting a recorder) ............................................................................. 12
2.6 (System self-test) ........................................................................................... 14
3 (Operation)...................................................................................................................... 15
3.1 (Getting started) ..................................................................................................... 15
3.2 / (On/Off) ............................................................................................................. 18
3.3 (Main menu overview)............................................................................... 19
3.4 (Operation the user interface) ................................................................ 20
3.5 (Preparing the system for run) .............................................................. 22
3.6 (Fluid handling components) ................................................................. 26
3.7 (Starting a run) .............................................................................................. 39
3.8 (During a run) ............................................................................................... 42
Set Buffer Valve Pos .................................................................................................................... 45
3.9 ( Post-run printing )................................................................................... 47
3.10 (Running a method template)................................................................ 49
3.11 ( Running a stored method)................................................................ 52
3.12 (Running the system manual)............................................................ 53
3.13 (Completion of a run and storage) .................................................... 56
3.14 (Restart after power failure) ............................................................. 57
3.15 (Cold room operation).................................................................................. 58
4 (Method programming)........................................................................................... 58
4.1 (General) .............................................................................................................. 59
4.2 programming line by line....................................................................... 59
4.3 ( Programming using method templates) ........................................... 66
4.4 (Editing a stored method).................................................................. 69
5 (Template description) ........................................................................................... 71
5.1 (General) ............................................................................................................... 71
5.2 (Application templates)................................................................................. 72

4
 (about this manual)

KTAprime

1 (Introduction)

1.1 (General)

KTATMprime
KTATMprime

50/ 1

5
KTATMprime UV
LCD pH

REC 112

A KTATMprime

1.2 (Description)

1.Fraction collector; 2. System pump;

3. Pressure senser; 4. Mixer; 5. Injection valve;
6. Buffer valve; 7. Gradient switch valve ,
8. Conductivity cell; 9. Optical unit; 10. Column;
11. Flow diversion valve;

KTATMprime

(Buff valve and gradient switch valve)

6
 (System pump)

(Pressure sensor)

(Mixer)

(Injection valve)

(Monitor)

UV pH

(Fraction collector with flow diversion valve)

1.3 (Safety)

UV
UV

2 6

N aOH

Amersham Pharmacia Biotech

Amersham Pharmacia Biotech

2 (Unpacking and installation)

2.1 (General)

KTATMprime KTATMprime

KTATMprime


+4 +40

(Installation procedure overview)

KTATMprime

9
2.2 (Pre-requisites)

KTATMprime

KTATMprime 120x80 x


0.1

KTATMprime 100-120/220-240 50-60

2.3 KTATMprime (Unpacking KTAprime)

120x80

1.

2.

10
3.

4.

5.

6.

7.

8.

9.

10. 5mm,

11.

12. 1

13.

11
14.

2.4 (Connecting mains cable)

1. KTATMprime

2.

3.

4.

5. KTATMprime

2.5 (Connecting a recorder)

REC112 ,

KTATMprime

12


1.

2. KTATMprime

mini-DIN Recorder
MC Rec.on/off ON/OFF

3.

4. (

1 () 1 0-1 V

2 () 0 V

13
 3 () 2 0-1 V

4 () 0V

5 () 3 0-1 V

6 () 0V

-------------------------------------------------------------------

1 - UV

2 - % B

3 -

Set Parameters

5. D-sub D-sub 12 ON/OFF 15

6.

7.

2.6 (System self-test)

Self test )

Please wait(
)
1

14
KTATMprime 2
V 1.00

3 30-40
Template( )

(Templates )

3 (Operation)

3.1 (Getting started)

KTATMprime

15


(Pre-requisites)

KTATMprime
1.3

(Typographical conventions)

(The system and software)

KTATMprime

KTATMprime UV pH

LCD

16


1. Buffer valve, 2. Buff, 3. Waste,

4. Fraction collector, 5. Waste,

6. Flow diversion valve, 7. Flow restrictor,

8. UV/conductivity/PH, / /(pH) 9. Injection Valve,

10. Pressure sensor, 11. Gradient switch valve

17
5 UV

pH pH

(flow restrictor)

6. l

3.2 / (On/Off)

Selftest() 1

Please wait()

2
KTAprime
V 1.00

3
Template(

4 30-40 Templates

18


3.3 (Main menu overview)

Esc

Templates

Templates.

3.7 Starting a run

Run Stored
Method

Run Stored Method 3.11

Running a stored method

Manual Run

Manual Run , 3.12

Running the system manually

Program
Method

Program Method

Method programming

Set
Parameters

SetParameters (UV)PH

C.2

Check

19
 Check

C.1

3.4 (Operation the user interface)

(Menu navigation)

OK OK

Esc Esc

1. 1 Main menu, 1 2. 2 Main menus 2, 3. ; press

or , 4. ; OK, 5. ; Esc, 6. 2.1; Sub menu 2.1, 7. 2.2. ;
Submenu 2.2, 8. 2.3 ; Submenu 2.3 9. ; Press or, 10. ; OK,
11. ; Esc, 12. ; OK , 13. ; Esc, 14. 2.1.1; Sub menu2.1.1, 15.
2.3.1; Sub menu2.3.1, 16. 2.3.2 ; Sub menu 2.3.2

20
 (control keys)

feed tube Set Delay UV

Frac

end

end

hold/cont

hold/cont

pause/cont

pause /cont

(Changing a parameter value)

21
 1. Parameter, 2. Current Value,----(15.0 /)

3. New value to be set, ---000

1 OK

3 OK Esc

3.5 (Preparing the system for run)

(General system preparation)

1 3.5

2 A1 B

22
 A1 A B B

3 NO

IN

4 4 5

5 , pH B.3Calibrating the PH electrode

(optional)

6 1 UV PEEK

(Unions and connectors)

8 2 6

3.6

9 30ml/

WASTE

23
 Set Concentration %B 100% B

10 1. 2.20%3.

4. A1

WASTE 50ml /

30ml pause/cont

System Wash Method 8.12

11 (Luer) /1/16 (injection fill port)

(Preparing the recorder)

1 Zero down

2 chart speed mm/s-mm/min (0.5-

2mm/min)

3 Pen down ( adjust)

4 rec. on

5 rec. off forward feed set

()
24
 6 zero up

7 (2-20 mm/min)

8 range V/m V 1 V

9 rec. on

REC 112

(Calibrations)

KTATMprime

B Calibration and analogue output setting

pH

25
3.6 (Fluid handling components)

(Columns and tubing)

KTATMprime

0.75 1.0

PEEK

(Recommended columns)

Ion Exchange Column name)

(Size Exclusion[Gel filtration]Columns)

26


(Hydrophobic Interaction Columns)

(Chelating Columns)

27
(Affinity Columns)

/ (Buffer Exchange/Desalting Columns)

(Sample application overview)

KTATMprime

28


25 -5

1 -150

>10

(Manual filling of sample loops)

(preparation)

1. (Luer female/1/16male union connector,/1/16"

) 3
29
 2. 4

3. 2 6

1; 1.Column, 2.Sample syringe 3. Waste

4.Sample loop 5. Pump

(injection fill port)

1. 3

2. 0.75

3.

4.

30
 5.

100 25 18-1113-98

500 10 18-1113-99

1 10 18-1114-01

2 10 18-1114-02

5 18-1140-53

50%

2-5

(Partial filling)

50%

1 LOAD

2
31
 3

2 ,

1.Column, 2.Sample syringe

3.Waste 4.Pump

4 INJECT

LOAD

50%

6 LOAD

LOAD

8 INJECT

32
 (Complete filling)

95% 2-3

1 LOAD

2 2-5

4 INJECT

(Emptying the sample loop)

(Applying Samples with Superloop)

1-150

33


10 4 18-1113-81

50 4 18-1113-82

150 2 18-1023-85

(Preparation)

1.Column, 2.Sample syringe,

3. Waste, 4. Superloop, 5. Pump,

1 /1/16 3

2 4

34
3

5 2

6 6

(Filling Superloop)

1 INJECT

3 LOAD

5 3

35
6 1

Set Sample Inj.Vol

Set Sample Ing. V0l.

(0.0ml.) 0.0

7 INJECT

LOAD

(Applying large samples with the system pump)

0.45

(Preparation)

1 0.45

2 8

3.5 Preparing

the system for a run

(Applying the sample)

36
1 Templates Method Template

LOAD

(Cleaning the pump)

NaOH

System Wash Method

1 NaOH 30

(Mixing gradients)

(Gradients)

(Mixer)

37
 0. 6 5 12

pH()

(Collecting fractions)

(Eluate fractionation)

Set Fraction Base Set Fraction Size OK

OK 0

Set Fraction Base

(ml) min ml drp

Set Fraction Size

(1.00 ml)

(Peak fractionation)

38


Set Peak Collect ,no

OK Set Slope OK

Set Peak Collect

(no)

Set Slope
(0.00 mAU/min) 0.00

C.2 Set Parameters menus

3.7 (Starting a run)

(Final checks)

Template
39
 Application Template System Wash Method

5 UV

6 pH ( ) B.3 Calibrating the pH electrode

(optional)

(Select an application template) KTATMprime

(Application templates)

(Method templates)

40
 3.10

(Stored methods)

3.11

(Manual run)

3.12

3.7.1.1 Tem
1 Template, OK

2 Application Template , OK
Applicatio

template 3 OK

41
 4 Sample Appl. Volume OK

Sample appl.Volume

0.0

5 Press ok to start run OK

Press OK to start run

(Tempate description)

3.8 (During a run)

(Viewing progrerss)

(Running display)

42


1 M=AT=MT=

M Run 10.0ml
20.0ml/min 1.10 MPa

Run

End

Pause

Hold

2 UV pH B /

0.00002AU pH8.50
20%B 22.90mS/cm

Cond 78.8% 22.4

Tube:01 Frac 5.0ml

4
43
 Waste V:(waste)
B V(1) IV:(waste)

(Printing Progress)

REC 112

1 UV

2 B%)

pH

Set Parameter Setup Analogue Out

RS-232

3 2 5

(Changing parameters)

( Pause/Cont )( Hold/Cont )

B (Changing the concentration of buffer B )

1 Set Concentration %B OK

44
 Set Concentration % B
(20%B) 30

2 OK

(Changing the flow rate)

1 Set Flow Rate OK

2 OK

Set Flow Rate

(0.1ml/min) 0.8

(Changing the fraction size)

1 Set Fraction Size OK

2 OK

Set Fraction Size

(00.0ml) 0.2

(Changing the buffer valve position)

1 Set Buffer Valve Pos OK

2 OK

Set Buffer Valve

Pos
(Pos 1) 1

(Changing the inject valve position)

1 Set Inject Valve Pos OK

2 OK

Set Inject Valve Pos

(Load) Waste Load Inject

45
Waste---( 4 5)

Load--- 3 ( 2 6)

Inject--- 1

(Autozero on the racorder)

1 Autozero OK

Autozero

(Setting an event mark)

1 Event Mark OK

Event Mark

3.12

(Interrupting a run)

End method?() end Y OK

n OK
End method
(Yes)
Yes no

pause/cont. pause/cont

hold/cont. hold/cont.

(Completing a run)
1 (Method complete) OK
Method Complete
Press OK to Continue

2 yes ( 3.9 Post-run printing)

no
46
 Memory Print Out
(no) yes
no

3.9 ( Post-run printing )

UV pH%B

Memory Print Out ?(?)

Memory Print Out?

(yes) yes no

1 yes on

2 Set Rec Out 1 1 OK

Set Rec Out 1 (UV)

UV pH Cond %B Tmp Pr

3 Set Rec Out 2 2 OK

Set Rec Out 2 (UV)

UV pH Cond %B Tmp Pr

4 Set Rec Out 3 3 OK

Set Rec Out 3(UV)

UV pH Cond %B Tmp Pr

47
5 UV yes , no

Autoscaling
(no) yes no

Cable wire no,( ) KTAprime signal (KTAprime )

1. 1 2. 3. 2 4.
5. 3 6.

7 set

8 OK

Set Parameters

1 rec. off

2 home

3 rec. on

4 pen. down

5 Set Parameters OK

48
 Set Parameters

6 Memory Print Out OK

Memory Print Out

7 Print out to Recorder OK

Print out to
Recorder Computer

8 2-5

3.10 (Running a method template)

KTAprime

(Selecting method template)

1. 3.5
2. Templates OK
Templates
3. Method Template OK
Method Template

4. OK

49
 HIC

(Setting the parameters)

B
1. 3.6

Sample inject by

InjV Pump
2. OK+ 0.2 Mpa()

Set Pressure Limit
(1.00 Mpa)
1.00
3. OK
Set Flow Rate
(--.- ml/min)
0.00
4. OK
Set Fraction Size
(0.0 ml)
0.0
5. 1 OK
Set Equilibr. Volume
(0.0 ml)
0.0
6. 2 OK
Set Sample Inj.Volume
(0.0 ml)
50
 0.0

7. 1 3 OK
Set Wash 1 Volume
(0.0 ml)
0.0
8. 4 OK
Set Elution.Volume
(0.0 ml)
0.0
9. 2 5 OK
Set Wash 2 Volume
(0.0 ml)
0.0
10. Method ready? yes OK
Methods ready?
(yes)
yes no
(Storing the method)
1. yes OK no OK
Save Method
(yes)
yes no
2. OK
Free
Used
FreeMethod
25
Sel.Method (Free)
16
free method OK Clear Method OK

Free Method
25
51
 Sel.Method (Used)
16
(Stsrting the run)
1. press Ok to start run OK
Press OK to start run

2. 3.8 During a run

(Finishing the run)
1. Method Complete OK
Method Complete
Press OK to continue
End yes OK
2. 3.9

3.11 ( Running a stored method)

KTATTMprime 40

(selecting a stored method)

1 3.5

2 Run Stored Method OK

Run Stored Method

3 OK
Run Stored Method
Number
13
(Starting the run)
1 Press Ok to start run OK

52
 Press OK to start run

3.8
(Finishing the run)
1 Method Complete OK

Method Complete
Press OK to continue

End Yes OK
2 3.9

3.12 (Running the system manual)

TM
KTA prime

(Preparing a menual run)

1 ( 3.5 )
2 Manual Run OK
Manual Run
(Setting the parameters)
OK
(Setting the method base)
1 Set Method Base. , OK
Set Method Base
)(ml)
ml
2 min ml OK
(Setting the concentration)
B
1 Set Concentration %B. OK
Set Concentration %B
(20%B)
30
2 OK
(Setting a gradient)
B
53
(
1 Set Gradient( off), OK
Set Gradient
(off)

2 B OK
Set Length
(0.00ml)
6.50

3 OK
Set Target
0(00%B)

Set Concentration %B.

(Setting the flow rate)

1 Set Flow Rate OK
Set Flow Rate
0.1ml/min)

2 OK
(Setting the fraction base)
1 Set Fraction Base OK
Set Fraction Base
(ml) min
ml drp
2 minmldrp OK
(Setting the fraction size)
1 Set Fraction Size OK
Set Fraction Size
(00.0ml)
0.2
2 OK
(Setting the pressure limit)
1 Set Pressure Limit OK
Set Pressure Limit
( ( 1.00Mpa)

2 OK+ 0.2 Mpa(

)
(Setting the buffer valve position )
1 Set Buffer Valve Pos. OK
Set Buffer Valve Pos

54
 (Pos 1)
1
2 OK
(Setting the injection valve position)
1 Set Inject Valve Pos. OK
Set Inject Valve Pos
(Load) Waste Load
Inject
2 OK

Waste---( 4 5)
Load--- 3 ( 2 6 )
Inject--- 1

(Starting the run)

1 Press OK to start run OK
Press OK to start run

( during a run)
(Viewing parameters)

3.8

(Changing parameters)

( Pause/Cont ) ( Hold/Cont )

55
 1. Set Concentration %B(20%B); %B(20%)
2. Set Gradient (off) ( )
3. Set Flow Rate(--.-ml/min) ( / )
4. Set Fraction Size (0.00ml) (0.00 )
5. Set Buffer Valve Pos (Pos1); ( 1)
6. Set Inject Valve Pos (Load) ( )
7. Autozero
8. Eventmark

OK
(Finishing the run)
1 Method complete OK
Method Complete
Press OK to continue
End yes OK
2 3.9

3.13 (Completion of a run and storage)

(Between runs)

System Wash Method

(Storage)

pH
pH pH 4 2
KNO3 11

UV
UV

56
 (Overnight)

pH pH

(Weekend and long term storage)

pH

pH 20%

20%( pH

UV 20%

pH (Storage of the PH electrode)

pH pH 4 2 KNO3 11

pH 4 2 KNO3 1

3.14 (Restart after power failure)

57
3.15 (Cold room operation)

(Preparation)

1 12

(Running)

2 pH

3 pH

(Removal from cold room)

2 12

4 (Method programming)

58
4.1 (General)

40 600

4.2 programming line by line

0 100%

%B

0 0 1 0 pos1
10 0 1 0 pos1
10S 0 1 0 pos1 /
20S 0 1 1 pos1 ,,

35S 100 1 1 pos1
49.9S 100 1 1 pos1
50S 0 40 0 pos1
70S 0 0 pos1
80S 0 1 0 pos1

59

1 Program Method OK
Program Method

25 16 ; m1; m1;

1.00MPa 0.00ml ); 10.0 ; %B Rec out 2

no yes no no yes no ; no yes no

Select a method number

1.
Free OK
Free Methods
25
Sel.Method (Free)
16
Used free OK
Free Methods
25
Sel.Method (Used)
16
OK
2. Method Occupied Clear OK
Method Occupled
(edit) edit
60
 clear

Clear Method yes OK

Clear Method 09
25
(yes) yes
no
General Parameter Setup
(Setting the method base)
1. Set Method Base OK
Set Method Base
(ml) min
ml
2. minml OK
(Setting the fraction base)
1. Set Fraction Base OK
Set Fraction Base
(ml) min ml
drp
2. minmldrp OK
(Setting the pressure limit)
1. Set Pressure Limit OK
Set Pressure Limit
(1.00 Mpa)
1.00
2. OK+ 0.2 Mpa()


(Setting breakpoints)

0.00

%B
61
1 0

(Selecting a breakpoint)
1. OK
Edit Breakpoint

0.00 ml
2 / OK
NEW OK
0.00

Edit Breakpoint

NEW
( Setting the concentration)
B
1. Set Concentration % B OK
Set Concentration %B
(20%B)
30
2. OK
B

0.1ml B

B
(Setting the flow rate)
1. Set Flow Rate OK
Set Flow Rate
(0.1ml/min)
0.8
2. 0.0 OK
62
(Setting the fraction size)
1. Set Fraction Size OK
Set Fraction Size
(0.00ml)
0.2
2. OK
(Setting the buffer valve position)
1. Set Buffer Valve Pos OK
Set Buffer Valve Pos
(Pos 1)
1
2. OK
(Settinh the injection valve position)
1. Set Inject Valve Pos OK
Set Inject Valve Pot
(Load) Waste Load
Inject
2. OK
Waste 4 5
Load 3 2 6
Inject 1
(Setting peak collection)
0 0
UV mAv/min

1. Set Peak Collect OK

Set Peak Collect
(no)
2. Set Slope OK
Set Slope
(0.00 mAU/min)
0.00
mAu/min ( 2.6 )
63
 10%

UV (Setting UV signal autozere)

1. Autozero OK
2. yes OK no
Autozero
(no) yes
no
(Setting an event mark)
1. Eveut Mark OK
2. yes OK, no
Event Mark
(no) yes
no
/ (Editing time/volume of a breakpoint)
1. Editing time/volume OK
Edit time/volume
( 12.7 ml)
2. Change Replace OK /
Edit time/volume
Change
Replace
Change /
Replace /
3. / OK
New time/volume
( 12.7ml)
(Saving the breakpoint)
Save Breakpoint OK
Save Breakpoint
(0.00min)
(Deleting a breakpoint)
Delete Breakpoint OK
64
 Delete Breakpoint
(0,00 min)
(Select an alarm)

1. Set Alarm
2. OK 26ml
26ml

Set Alarm at
(No alarm)
26.00
(Printing the method)
2 B :
1. Show %B on Rec out 2
Show %B on Rec out 2
(no) yes
no
2. yes OK%B
(Ending the method)

1. End Method
End Method
(yes) yes
no
2. yes OK
3.
Edit Breakpoint

0.00ml
(Saving the method)

1. Save Method
65
2. yes OK
Save Method
(yes) yes
no

4.3 ( Programming using method templates)

0 100%

%B

10 0 F 0 pos 1 1.
S 0 F 0 pos 1 2.
10 0 F 0 pos 1 3. 1
15 100 F 1 pos 1 4.
15 100 F 1 pos 1 5. 2
(
20 0 F 0 pos 1 )

(Selecting method template)
1. Templates OK
Template
2. Method Template OK
Method Template
3. OK

66
 / / HIC / /

5 (Yemplate description)
4.
(Setting the parameters)
1. 3.6
Fluid handling Components
Sample inject by
InjV Pump
2. OK+ 0.2Mpa()


Set Pressure Limit
(1.00Mpa) , 1.00
3. OK
Set Flow Rate
(--.- ml/min)
0.00
4. OK
Set Fraction Size
(0.0 ml)
0.0
5. 1 OK
Set Equilibr. Volume
(0.0 ml)
0.0
6. 2 OK
Set Sample Inj.Volume
(0.0 ml)
0.0
7. 1 3 OK
67
 Set Wash 1 Volume
(0.0ml)
0.0
8. 4 OK
Set Elution. Volume
(0.0ml)
0.0
9. 2 (5.) OK
Set Wash 2 Volume
(0.0 ml)
0.0
10. Method ready? yes OK
Method ready ?
(yes)
yes no
(Storing the method)
1. yes OK
Save Method
(yes)
yes no
2. OK
Free Methods
25
Sel. Method (Free) 16

Free Methods
25
Sel. Method (Used) 16
Free
Used ,
OK Clear Method OK,
,.

68
4.4 (Editing a stored method)

, ,, 4.2

(Selecting method)
1. Program Method OK
Program Method
2. OK
Free Methods
25
Sel. Method (Used)
08
3. yes OK
Method Occupied
(edit) edit
clear
( 4.2 )
(Editing an existing breakpoint)
1. Edit Breakpoint
Edit Breakpoint

0.00 ml
2.
3. OK,
4.
5. OK
Save Breakpoint
(0.00 ml)
/(Editing time/volume of breakpoint)
1. Edit breakpoint
Edit Breakpoint

69
 0.00ml
2.
3. OK,
4. Edit time/volume OK
Edit time/volume
( 12.7ml)
5. Change Replace OK /
Change /
Replace /
Edit time/volume
Change
Replace
6. / OK
New time/volume
( 12.7ml)
15.0
(Inserting a breakpoint)
1. Edit breakpoint
Edit Breakpoint

0.00 ml
2. New
Edit Breakpoint

New
3. New OK 0.00
4. OK
5.
6. Save Breakpoint OK
Save Breakpoint
(0.00 ml)
(Deleting a breakpoint)
1. Edit breakpoint
70
 Edit Breakpoint

0.00 ml
2.
3. OK
4. Delete Breakpoint OK
Delete Breakpoint
(0.00 ml)
5. yes OK
Delete Breakpoint

yes
(Printing the method)
2 ()
1. Show %B on Rec out 2
Show %B on Rec out 2
(no) yes
no
2. yes OK,
(Saving the method)
,
1. Save Method
Save Metnod
(yes) yes
no
2. yes OK

5 (Template description)

5.1 (General)

71
 TM
KTA prime

5.2 (Application templates)

(General)

His-
GST-

IgM

:
1. Templates OK
Templates
2. Application Templates OK
Application template
3.
,
, 3.7 (Starting a run)
,.
.
S

72
 HiTrap (HiTrap desalting)
HiTrap .
, Desalting/HiTrap Desalting OK
Desalting
HiTrap Desalting
: HiTrap 5ml
9

%B
0 0 45 0 pos 1 A1
35 0 5 0 pos 1
60
60 0 5 0 pos 1
60+S 0 5 1 pos 1
75+S

HiPrep (HiPrep desalting)

HiPrep .
, Desalting/HiPrep Desalting OK
Desalting
HiPrep Desalting
: HiPrep 26/10
18

%B

0 0 40 0 pos 1 A1
73
35 0 20 0 pos 1
300
300 0 20 0 pos 1
300+S 0 20 5 pos 1
400+S

His-(His-tag purification)
His- His-
, His-tag purification /HiTrap Chelalting OK

His-tag Purification
HiTrap Chelating

: HiTrap Chelating 1ml

77

%B

0 100 40 0 pos 2 B
25 100 40 0 pos 2 B
25+0.1 0 40 0 pos 2 A2
74
60 0 1 0 pos 2
65 0 40 0 pos 3 A3
100 0 1 0 pos 3 Ni
101 0 40 0 pos 2 A2
116 0 1 0 pos 2
121 0 40 0 pos 1 A1
156 0 1 0 pos 1
166
166 0 1 0 pos 1
166+S 0 1 0 pos 1
176+S 0 1 1 pos 1
196+S 100 1 1 pos 1
213+S 100 1 0 pos 1
213.1+S 0 40 0 pos 2 A2
228+S 0 1 0 pos 2
233+S

GST-(GHT-tag purification)
GST- GST-.
, GST-tag Purification/GS Trap OK
Gst-tag Purification
GSTrap
: GSTrap 1ml
37

%B

0 0 40 0 pos 1 A1
35 0 1 0 pos 1
45
45 0 1 0 pos 1
45+S 0 1 0 pos 1
55+S 0 1 0 pos 1
55.1+S 100 40 0 pos 1 B
90+S 100 1 1 pos 1
100+S 100 1 0 pos 1
100.1+S 0 40 0 pos 1 A1
115+S 0 1 0 pos 1
75
120+S

Mab () [Mab purification (step elution)]

Mab ()
, Mab purification/Step Elution OK
Mab Purification
Step Elution
: HiTrap protein G 1ml ( HiTrap protein A HiTrap rprotein A 1ml)
: 37
GST-tag
(Albumin removal)

, Albuin Removal/HiTrap Blue OK
Albumin Removal
HiTrap Blue
: HiTrap Blue 1ml
: 37

%B

0 0 40 0 pos 1 A1
35 0 1 0 pos 1
45
45 0 1 1 pos 1
45+S 0 1 1 pos 1
55+S 0 1 0 pos 1
55.1+S 100 40 0 pos 1 B
90+S 100 1 1 pos 1
100+S 100 1 0 pos 1
100.1+S 0 40 0 pos 1 A1
115+S 0 1 0 pos 1
120+S

76
 Mab () [Mab purification (gradient elution)]
Mab () PH
, Mab purification/Gradient elution OK
Mab Purification
Gradient Elution
: HiTrap protein A 1ml ( HiTrap protein A HiTrap rprotein G
1ml)
: 63

%B

0 100 40 0 pos 1 B
25 100 40 0 pos 1 B
25+0.1 0 40 0 pos 1 A1
60 0 1 0 pos 1
70
70 0 1 0 pos 1
70+S 0 1 0 pos 1
80+S 0 1 1 pos 1
100+S 100 1 1 pos 1
117+S 100 1 0 pos 1
117.1+S 0 40 0 pos 1 A1
132+S 0 1 0 pos 1
137+S

(Anion exchange)

, Anion exchange/HiTrap Q OK
Anion Exchange
HiTrap Q
: HiTrap Q 1ml
: 63
Mab ()
(Cation exchange)

, Cation exchange/HiTrap SP OK
77
 Cation Exchange
HiTrap SP
: HiTrap SP 1ml
: 63
Mab ()
IgM (IgM purification)
IgM IgM
, IgM purification / HiTrap IgM purification OK
IgM Purification
HiTrap IgM purification
: HiTrap IgM purification 1ml
: 48

%B

0 0 40 0 pos 1 A1
35 0 1 0 pos 1
45
45 0 1 1 pos 1
45+S 0 1 1 pos 1
55+S 0 1 0 pos 1
55.1+S 100 40 0 pos 1 B
90+S 100 1 1 pos 1 1
100+S 100 1 0 pos 1 1
100.1+S 0 40 0 pos 2 A2
135+S 0 1 1 pos 2 2
145+S 0 40 0 pos 1 A1
160+S 0 1 0 pos 1
165+S

(Refolding)

, Refolding / HiTrap Chelating OK
Refolding
HiTrap Chelating
78
 : HiTrap Chelating 1ml
: 112 .

%B

0 100 40 0 pos 5 B
25 100 40 0 pos 5 B
25.1 0 40 0 pos 5 A5
50 0 40 0 pos 2 A2
85 0 1 0 pos 2
90 0 40 0 pos 3 A3
125 0 1 0 pos 3 Ni
126 0 40 0 pos 2 A2
141 0 1 0 pos 2
146 0 40 0 pos 1 A1
181 0 1 0 pos 1
191
191 0 1 0 pos 1
191+S 0 1 0 pos 1
201+S 0 40 0 pos 1 A4
236+S 0 1 0 pos 1
246+S 0 0.5 0 pos 1
276+S 100 0.5 0 pos 1
286+S 100 1 1 pos 2
306+S 0 1 1 pos 2
323+S

(System wash)
.
1 , System Wash Method OK
System Wash Method

2 OK
Select Buffer V. pos
B, A : 2,3,_,_,_,_,_,_,_,
OK
: A1 ,

79
 %B

0 100 50 0 pos 8 B
24.9 100 50 0 pos 8 B
50 0 50 0 pos 8 A8
75 0 50 0 pos 7 A7
100 0 50 0 pos 6 A6
125 0 50 0 pos 5 A5
150 0 50 0 pos 4 A4
175 0 50 0 pos 3 A3
200 0 50 0 pos 2 A2
225 0 50 0 pos 1 A1
250 0 50 0 pos 1
275 0 1 0 pos 1
276 0 1 2 pos 1
278

5.3 ( Method template )

(General)
,,
/ .



:
1. Templates OK
Templates
2. Method Templates OK
5.3.1.1.1.1.1 Method
template
,.,
,
,

, 3.10 (Running a method

template)
80
 ..

/ (Gelfiltration / buffer exchange)

, Grelfiltration / Buffer Exchange OK
Gelfiltration / buffer
exchange

1
2
3

(Ion exchange )
, Ion Exchange / Gradient Elution OK
Ion Exchange
Gradient Elution

1
2
3 1
4
5 2
A A (20ml,40ml/)
B (=)

81
 HIC (hydrophobic interaction chromatography)
, HIC/Gradient Elution, OK
HIC
Gradient elution

(Affinity)
, Affinity / Step Gradient OK
Affinity
Step Gradient

1
2
3 1
A A (15ml,40ml/)
4
B 2 (15ml,40ml/) 2

5 2 ()
C A (15ml,40ml/)
D (= )

6 (Handling components)

6.1 (General)

TM
KTA prime

82
 UV

pH

6.2 (Installing the optical unit)

UV (Changing UV flow cell)

(2 5
2 5
5 2

1. ; Protective cover for

flow cell
2. O- ; O-ring , 2 ,5

83
 5

1. ; Detector housing
2. ; Locking nut

(Connecting the optical unit in the system)

1

1. ; Screw 2. ; Holder
3. ; Optical unit

2
3 UV Lamp
4 UV

(Connection to the column)

1

84
 3 .
PEEK ( 0.75 , 1/16)
170
4

[Changing the lamp assembly(optional)]

UV
UV

Lamp housing and plate

2
3 1
4

5
6
280 Zn

1. ; Dot on lamp housing

2. ; Symbols on filter housing
3. ; Symbol on filter wheel
below lid

85
 [Changing the filter (optional)]
Hg 254 280 Zn 214

1 Zn ()
2

60

5
3

; Triangular aperture

6
7
8

10
86

11

6.3 Installing the conductivity cell)

1 /

1.5

1. ; Holder 2. ; Conductivity cell

3. ; Clamp

pH

pH
2
3

6.4 (Installing the fraction collector)

(Assembling the tube rack)

87


12 175 12 50-180

18 95 10-18 50-180

30 40 30 30-180

12 30 12 Eppendorf (18-
8522-01) 1.5 Eppendorf 0.5
TM
KTA prime 18

:1. ; Single cutout 2. L- ; L-shaped

cutout 3. ; Tube holder 4. ; Tube guide
5. ; Tube support 6. ; Bowl

L-

12 28 ( Tube racks,12 and 18mm)

50-85 85-100
L-
L-

88
 38 (Tube rack, 30mm)
30-50 50-85 85-100
L-
L-

30-50 30 18

2 1
3 1 1

(Mounting the tube rack)

89
 ; Drive Sleeve

(Inserting the collection tubes)

1

(Adjusting the delivery arm)

1

Tube sensor

2 5

Lock knob

; Sensor control

4 1
1
5

(Connecting tubing)
0.75mm 1-5
90
1 41

; Tubing holder

1 ;Sensor control 2 ;
Tube sensor

3 NC

(Flow diversion valve)

IN

NO
91

NC

6.5 pH () [Installing the pH flow cell and

electrode (optional)]

(Mounting the flow cell holder)

TM
KTA prime pH

1
30

Flow direction

pH (Inserting the pH electrode)

pH

pH
pH 4 2 KNO3 11

92
1 pH
2 30

1. ;To system rear panel 2. ; Electrode

3. ; Flow cell holder 4. ;Flow cell 5. ;
End cover

4 pH pH-Probe

7 (Maintenance)

7.1 (Periodic maintenance)

TM
KTA prime

93


N aOH

Amersham Pharmacia Bioech

Amersham Pharmacia Bioech

pH
pH 20%
20%


96%
pH Calibrating the pH electrode pH

7.4
0.2 + 0.05

1
2 1
3 10 /
4
5 10 /
6
94
 6

7.9 7.10 UV
pH 7.14

7.6
O-


7.16

7.8

7.16
O- 4

7.11
7.13 pH

7.2 (Cleaning the system)

0.75 PEEK

(At the end of the day)

System Wash Method

1
2 Application Template System Wash Method
3 OK

(Leaving the system for few days)

pH
pH pH 4 2M KNO3
11

System Wash Method 20% pH

95


pH [PH electrode (optional)]

pH pH 4 2M KNO3 11
pH

(Monthly cleaning)

NaOH

1
2 1 NaOH
3 System Wash Method
4 1 NaOH 20 1 /
5 3 4 NaOH

(Other cleaning considerations)

7.3 (Moving the system)

7.4 UV (Checking the UV monitor)

96
 (Checking lamp intensity)
1 Check OK
2 Check Lamp Intensity
Check Lamp Intensity
R 215.5 S 214.7mv

254 R<300
280 R<150
214 R<150
7.15 UV Amersham Pharmacia Biotech

(Checking lamp run time)

1 Check OK
2 Check Lamp Run Time
Check Lamp Run Time
Hg 2300h Zn 340h
254 7000 2000

280 3500
Zn 2000
7.15 UV Amersham Pharmacia
Biotech

(Checking autozero)

1 Check OK
2 Check autozero
Check Autozero
AZ 0.0001AU

7.5 (Checking the pump)

(Checking pump run time)

1 Check OK
2 Check Pump Run Time
Check Pump Run Time
00014h

1000
Amersham Pharmacia Biotech

97
 (Checking pumped volume)
1 Check OK
2 Check Pumped Volume
Check Pumped Volume
194529452ml
Amersham Pharmacia Biotech

7.6 (Checking the fraction collector)

(Checking tube shifts)

1 Check OK
2 Check Tube Shifts
Check Tube Shifts
17564
Amersham Pharmacia Biotech

7.7 (Checking the rotary valves)

1 Check OK
2 Check Valve Shifts
Check Valve Shifts
BV17564 IV:28143

BV IV
>50,000
7.8 Changing the channel plate and distribution plate on
rotary valves AmershamPharmacia Biotech

7.8 (Changing thechannel plate and

distribution plate on rotary valves)

98
 1. ; Distribution plate 2. ; Channel plate
3. ; Injection valve 4. ; Buffer valve

1 1
2 3 L (Alley key)

3
4
5
6 text 3() i/o (
L

7.9 UV (Cleaning the flow cell in-place)

NaOH

1 NaOH 30

7.10 UV (Cleaning the UV flow cell off-line)

UV

1 10%
Decon 90Deconex 11RBS 25
99

2 20
3
4 10

7.11 (Cleaning the conductivity flow cell off-line)

NaOH

1 1 / 15 1
NaOH
2 15
3

0.8

7.12 (Changing the conductivity cell)

/

B.2 Calibrating conductivity
,( B.2 Calibrating conductivity)

7.13 pH [Cleaning the PH electrode (optional)]

pH 6 <80%

NaOH

0.1 HCl 0.1 NaOH

0.1 HCl 5


0.1 HCl 1% 5

100

1 1 KNO3 60-80
2 KNO3
3 KNO3

7.14 pH [Changing the PH electrode(optional)]

6.5 pH ()

7.15 UV (Changing the UV lamp)

254 Hg 7000 2000

280 Hg 3500

Zn 2000

UV UV


1
2

1. ; Unscrewing the lamp

2. /; Removing/inserting the lamp

4 Set Parameters OK
5 Setup and Calibration OK
6 Setup UV OK
7 Set Lamp Run Time OK
8 Lamp Run Time 0, OK
101
 9 Esc

7.16 (Replacing the mixer chamber)

1.
2.
3.
4.
5.
6.
7.

1. ; Outlet 2. Spring 3.
Chamber lock 4.. Laft-hand inlet 5.
Right-hand inlet

8 (Trouble-shooting)

8.1 (Faults and actions)

GE Healthcare
GE Healthcare

AKTAprime
V 1.00

102
8.2 (System)

8.3 UV (UV curve)

UV 1 Check Autozero
1.5 2

2

3
4
0.20.05
5
6
7 UV 7.9 7.10
8

9 8.12

1
2 7.2
3
4
5 FR-904 FR-
902 0.4 0.2

1
2

103
8.4 (Conductivity curve)

1
2
3

4
5

1
0.2 +0.05
2
3
4
5 7.11

1 7.11
2

1
2
3

1 pH
2
3 1.00 NaCl

1
2

%B 1
2

8.5 pH [PH curve (optional)]

104

pH 1
2
3

4
20 /
30 pH
5 pH
6 pH
7 80-105%
0 60 pH
pH
8 pH 7.13
9 pH

10 4
pH
11 pH
12 pH
pH

13 5,14

pH 1
2

pH 1 7.13 pH
2 pH

pH 1 7.13

2

3 7.13

pH 1 pH

8.6 (Pressure curve)

105

1
2

1
2
3
4 8.12

1
2
3

8.7 (System pump)

1
2
3 8.12

8.8 (Mixer)

1
2

8.9 (Fraction collector)

1 Feed tube GE Healthcare

2 Feed tube


GE Healthcare

1 8.13

106
 1

8.10 (Buffer valve and injection valve)

1 i/o 3 (

1 7.8

1
2 7.8

8.11 (Error messages)

GE Healthcare

34 Start up failed
Retry/Call service
1
2

34WARNING wrong
averaging time set
1 Set parameters

50 Electrical error
Call for service

57 Electrical error
Call for service

75 Electrical error
Call for service
1

107
60 Tube switch
always active
1
2
3

61 No more tube is
available
1

62 Check that the tube

position is OK
1
2
3

64ERROR No drops
check sensor
1
2 Drop
sync
3

65 ERROR
Pumpfailure
1
2

66 Toop short time

between feeds
1

67 ERROR injection
valve falure
1

68 ERROR Buffer
valve falure
1

69 ERROR Stop grad

set HOLD or PAUSE
1 HOLD PAUSE(%B)

70 Lamp disconnected
if not,call service
1

108
71WARNING low light
intensity
1
2
3

72 Change lamp or
call service

76Change lamp or
call service
1
2
3
73 WARNING Too much
straylight leaks in
1
2 UV
3
4

77WARNING Autozero
out of range
1 2
2
3 UV

78 ERROR
over pressure
1

79 ERROR Method
corrupt in eeprom
1 ()
2

80ERROR
Reading from eeprom
1

81ERROR
Writing to eeprom
1

82 ERROR Parameter
fail in method
1
109
83 WARNING temp-cal
will be changed

84 WARNING cond-cal
Will be changed
1 OK
2 ESC

85 WARNING conscale
(0-100%)< 0.1ms
1 0% 100% 0.1ms/cm
2
SetParameters

86WARNING cond-cell
bad/not connected
1
2
3

87 WARNING pH-probe
bad/not connected
1 pH
2 pH
3

88 Electrical error
call for service
1 pH
pH
2

89 Electrical error
Call for service
1 pH

2

90 ATTENTION
set <=0mv first

91 WARNING
bad pH and value
1

92 WARNING electrode
slope<70 or >110%
110
93 pH-cal failed
check electrode
1
2 pH
3

94 WARNING < 1pH unit

between cal-buff 1&2
1 pH pH

95 Temp cal failed

check cond cell
1
2 +5C
-8C

97 WARNING pH scale
(0-100%)< pH unit
1 pH pH
SetParameters

98 Cal failed.Cell
constant not 0,1-300
1
2 1.00M NaCl
3

4
5

99 ERROR Out of
method memory
1 600

ERROR key
1
2
3

ERROR Number 102-104

ERROR Number 109-113

111
 ERROR Number 119-121
1
2
3

Exc x/y in ab.c

Exc DIV/0 in ab.c

Exc instr in ab.c

Exc address in ab.c

1
2
3

8.12 (Removing trapped air bubbles)

1. 2. 20%3.
4.

A1 WASTE
50ml/ 30ml. pause/cont

1 4
2 3 20%
3 2
4 1
5
6 Templates Application template System Wash Method
7 23 4 1 B
8 OK

112
8.13 (Adjusting the spring tension of the
delivery arm)

1
2
3


4 1/4

9 (Reference information)

9.1 (System description)

A.1 (The system)

113
KTAprime UV pH

A.2 (Components)

1. ;Fraction collector 2. ;System pump

3. ;Pressure sensor 4. ; Mixer 5. ;Injection valve
6. ; Buffer valve 7. ; Gradient switch valve
8. ;Conductivity cell 9. ;Optical unit 10. ;Column
11. ;Flow diversion valve

114
A.2.1 (Buffer valve)
8 360
1-8

PEEK

1. Central port 2. Peripheral port

A..2.2 (Switch valve)

3- / 2- 24 COM
NC NO
COM

1. ; Inactive 2. ; Active

A.2.3 (System pump)

50 / 1.0

120

PEEKKalrez,Simriz,ceramic (Polyethylene)

A.2.4 (Pressure sensor)

115
A.2.6 (Mixer)
2

1 22
2

600 /

A.2.6 (Injection valve)

(memory effect) PEEK

A.2.7 (Monitor)
UV pH
214 Zn-254
280 Hg-

116
A.2.8 UV (UV optical unit)

UV Zn Hg UV

2 5 ()

2 5 UV
UV flow cells with 2 and 5 mm path lenghts

6 2

1.; Optical Unit 2. ; Lamp 3. ; System housing

4. ; High voltage power supply 5. ; Front panel 6. ; Microprocessor
7. ; Photodetector 8. ; Inlet 9. ; Flow cell 10. ; Outlet
11.; Beam splitter 12. ; Lens 13. ; Filter 14. ; Potodetector

Hg 280
280
214
Zn Hg

A.2.9 (Conductivity flow cell)

50 50

117

B.2

1. ; Eluent 2. ;Temperature
sensor

A.2.10 FR-902(Flow restrictor FR-902)

FR-902 0.2

A.2.11 (Fraction collector)

12 175
10-18 95 30 40

5 /

A.3 (Electrical connections)

KTAprime

118
A.3.1 (Mains cable)

A.3.2 (Communication cables)

1.Rs-232 Rs-232 to computer 2. To flow diversion valve

3.To fraction collector 4. Measurement data to recorder
5. On/off-signals to recoder 6. To UV lamp (high
voltage)
7. From optical unit 8. From conductivity flow cell
9. PH From PH-electrode

A.4 (Fluid handling path)

KTAprime

119
 1. ; Mixer 2. ; Stop plug 3. ; Pressure monitor 4. ; System
pump
5. ; Gradient switch valve 6. ;Buffer valve 7. ; Injection valve
8. ; Fraction collector 9. ; Fraction switch valve 10. 0.2 ; Flow
restrictor 0.2Mpa 11. /; Male/Male 12. ; Column 13. 100 ; Loop 100l

KTAprime

10
2.9 3/16 Teflon 3.4Mpa 660.5 l
(A1, ,B)
1.6 1/8 Teflon 2 Mpa 201.1 l
(AB)
1.0 1/16 PEEK 4.1Mpa 78.5 l (W1-W3)
0.75 1/16 PEEK 2.6Mpa 44.2 l
(G1-G6F1)
1/16PEEK ( 25 Mpa --
)
1/16PEEK -- --

A.5 (Optional components)

A.5.1 pH [PH flow cell with electrode (optional)]

pH Ag/AgCl
Ag / AgCl 4 KCl 1 KNO3
pH .pH

120
 1. ; Sealing 2. AgCl 4 KCl ; Internal
electrolyte bridge containing 4M KCl saturated with AgCl 3. t ;
Silicone sealing 4. Ag/AgCl ; Ag/AgCl reference electrode 5.
; Internal annular coaxial ceramic reference junction 6. 1
KNO3 ; External electrolyte bridge containing 1M KNO3 7.
O- ; O-ring 8. KCl ;Glass electrode containing
diluted buffered KCl 9. ;External annular coaxial
ceramic reference junction

pH

A.5.2 (Superloop)

1050 150
1-101-50 1-150

9.2 (Calibration and analogue output settings)

Set Parameters

121
B.1 (Calibrating the system pump)
B1.1 (Calibrating the flow rate)

1
2 A1
3
4 Setup and calibration Start Pump Calibration OK
5 Set Flow Rate 2ml/ OK
5
7 Enter Collected Volume OK
8 Pump Calibrated ok OK

B.1.2 (Calibrating the pressure offset)

1
2 Setup and calibration Change Press Offset, OK
3 Set zero pressure to calib. OK
4 Calibration Offset Donr OK

B.2 (Calibrating conductivity)

C.2.11 Setup adjust cell
constant
C.2.10 Setup
adjust conductivity .
B.3 PH () [Calibrating the PH electrode(optional)]
pH
.pH pH
pH 1 pH . pH

B.3.1 (Calibrating with the electrode outside the flow

cell)

1. ( pH7)
122
2. Set parameters OK
Set Parameterst
3. Setup and Calibration OK
Setup and calibration
4. Setup pH OK
Setup pH
5. Calibrate pH. (buffer1-buffer2)
Calibrate pH (7.00-12)
Buffer1Fixed lower Calibrated pH Value. Range0.00-14.00
[ 1= pH =0.00-14.00]
buffer2Fixed higher Calibrated pH Value. Range0.00-14.00
[ 2= pH =0.00-14.00]
: 1 2 pH
6. OK 1 2 . OK 1
2 TBA 1
Calibrate pH buffer 1

Calibrate pH buffer 1
(7.00) Please
wait!
7. ,
Calibrate pH buffer 1
(7.00)
7.00
8. pH pH ,
OK
9. 2 (
pH4.0 9.0), OK
Calibrate pH buffer 2

Calibrate pH buffer 2
(9.00) Please
wait !
123
10. .
Calibrate pH buffer 2
(9.00)
9.00
11. pH pH OK
12. 2 Calibrated Electrode
100% 25 pH 59.16mv pH7
mv ESC Set Parameters

Calibrated Electrode
Slope 98.5%
9.5mV

13.
95%-102% +30mV ,

,+60mv 80%,
.

B.3.2 (Calibrating with the electrode in the flow cell)

30-50 ml ( 2ml )
pH
B.4 (Setting analogue outputs)

pH
,()

124
1. Set Parameters OK

Set Parameters
2. Setup Analogue out OK
Set Analogue Out
B.4.1 (Setting parameters for the channels)
pH B ,

1. Setup Analogue out 1

2 3
Set Rec Out 1
(UV)
2. OK 1
Set Rec Out 1
(UV)
UV pH Cond%B Temp Pr
3. OK
:Temp()0 0V,50 1.0V
B.4.2 UV (Setting the UV analogue output)
1. Setup Analogue out Setup UV Analogue out
() 0.0001, 0.0002,
0.0005, 0.001, 0.002, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1.0, 2.0
5.0
Set UV Analogue out
(0.005 AUFS 10%)
2. OK , OK
Set UV Zero Level
(10.0%)
3. 0 , OK
Set UV Zero Level
(10.0%)
10.0
4. OK
125
 Set UV Range
(0.0005 AUFS)
5. OK
Set UV Range
(0.0005 AUFS)
0.0002
B.4.3 (Setting the cond analogue output)
1. Setup Analogue out Set Cond Analogue out ,
()
Set Cond Analogue out
(00.00-50.00 mS/cm)
2. OK . OK
Set Cond Zero Level
(0.00mS/cm)
3. 0 , 0.000-999.9ms/cm OK
Set Cond Zero Level
(0.00mS/cm)
0.00
4. ., . OK
Set Cond Full Scale
(50.00mS/cm)
5. , 0.000-999.9ms/cm OK
Set Cond Zero Levell
(50.00mS/cm)
50.00
6. Esc Set UV Analogue Out
B.4.4. pH (Setting the pH analogue output)
: pH pH .
: , .
1. Setup Analogue out Setup PH Analogue out ,
()
Setup pH Analogue out
(pH 0.00-14.00)
126
2. OK ,. OK
Set pH Zero Level
(pH 0.00)
3. 0 , pH-0.50-14.30 OK
Set pH Zero Level
(pH 0.00)
00.00
4. ,, OK
Set pH Full Scale
(pH 14.00)
5. , pH-0.50-14.30. OK

Set pH Full Scale

(pH 14.00)
14.00
6. ESC Setup Analogue out
B.4.5. (Setting the Press analogue output)
1. Setup Analogue out Set Press Anologue out
, ()
Set Press.Analogue Out
(1.00 Mpa)
2. OK 0.00-1.00Mpa OK .
Set Press Analogue Out
(1.00 Mpa) 2.00

9.3 (Menus)

C.1 (check menus)

C.1.1 (Checking autozere level)

1. Check OK
Check Autozero
127
 AZ
0.00006 AU
2. Check AutoZero .
C.1.2. (Checking lamp run time)

1. Check OK
2. Check Lamp Run Time
Check Lamp Run Time
Hg 1482h Zn 430h
C.1.3. (Checking lamp intensity)

1. Check OK
2. Check Lamp Intensity
Check Lamp Intensity
R 215,5 S 214.4mV
C.1.4 (Checkingh pump run time)

1. Check OK
2. Check Lamp Run Time
Check Pump Run Time
246h
C.1.5 (Checking pumped volume)

1. Check OK
2. Check Pamped Volume
Check Pumped Volume
3567 ml
.1.6 (Checking tube shifts)

Check ok
Check Tube Shifts
Check Tube Shifts
3592
.1.7. (Checking valve shifts)

128
 Check OK
Check Valve Shifts
Check Valve Shifts
BV: 642 IV:348
.1.8 (Checking recorder)

1. Check OK
2. Check Recorder
Check Recorder
3. OK

0

4. OK

.1.8 (Checking service mode)

.
1. Check OK
2. Check Service Mode OK
Check Service Mode
3. OK
Telephone Service:
012345678901
4. OK
Contract number:
01234567801
5. OK
Serial Number:
0123456 YM012345
6. OK
KTAprime
V1.00

7. OK
Date of maintenance:
DD MMMM YYYY
8. OK
Buzzer Test

.2 (Set Parameters menus)

C..(Switching the lamp on / off)
60
129

1. Set Parameters OK
2. Lamp OK Setting
Lamp
(on)
3. OK
Lamp
(on)
on off

..(Setting drop synchronization)

,

ml/min

1. Set Parameters OK
2. Set Drop Sync Active
OK
Set Drop Sync Active
(yes)
3. OK

..(Memory print out)

p-

1. Set Parameters OK
2. Memory Parameters Out OK
Memory Print Out
3. OK
Print out to?
Recorder Computer
4. OK Setting
Set Rec Out 1
(UV)
5. OK

Set Rec Out 1

(UV)
UV pH Cond %B Tmp Pr
6.

7. UV- OK

Autoscale UV
(no)
130
8. OK
Autoscale UV
(no)

Yes no

.. (Setting analogue outputs)

.4

.. p (Setup calibrate pH )
.3 p

.. p (Setup pH temperature compensation )

p p
p
p

Set Parameters OK
Set Parameters

2. Setup and Calibration OK

Setup and calibration
3. Setup pH OK
Set up pH
4. Set pH Temp Comp p on
off OK

Set pH Temp Comp

(off)
5. OK
Set pH Temp Comp
(off) on off

.. p(Setup show pH)

pp off

1. Set Parameters OK
Set Parameters
2. Setup and Calibration. OK
Setup and Calibration
3. Setup pH OK
Setup pH

131
 4. Set Show pH p on p
off p OK
Set Show pH
(on)
5. OK
Set Show pH
(off) on
off

.. (Setup conductivity temperature compensation)

1. Set Parameters OK
Set Parameters

2. Setup and Calibration OK

Setup and Calibration

3. Setup Cond OK
Setup Cond

4. Set Cond Temp Comp OK.

off.0%9.9%
Set Cond Temp Comp
(0.0%)

5. OK
Set Cond Temp Comp
(0,0%)
0.0

.. (Setup conductivity reference temperature)

1. Set Parameters OK
Set Parameters

2. Setup and Calibration OK

Setup and Calibration

3. Setup Cond OK
Setup Cond

4. Set Cond Ref Temp OK

0.00~99. 9
Set Cond Temp Comp
(25.0)

5. OK
Set Cond Temp Comp
132
 (25.0 )
25.0

.. (Setup adjust conductivity)

Set Cond Temp Comp 0(

..)..

1. 1 .00 NaCl 58.44g/L

2. ml
3. -
4.

1.00M NaCL 20-30C

(C)
(mS/cm)

133


5. Set Parameters OK
Set Parameters
6. Set and Calibration OK
Setup and Calibration
7. Setup Cond OK
Setup Cond
8. Set Adjust Cond OK
Set Adjust Cond
(80.32mS/cm)
9. OK
Warning! This will change cell calibr.
10. OK
.000-999.9ms/cm
Set Adjust Cond
(80.32 mS/cm)
83.55

.. (Setup adjust cell constant)

1. Set Parameters OK
Set Parameters
2. Setup and Calibration OK
Setup and Calibration
3. Setup Cond OK
Setup Cond
4. Set Adj Cell Cont OK

Set Adj Cell Const

-1
(83.56 cm )

5. OK
Warning! This will change cell calibr.

Set Adj Cell Const

-1
(83.56 cm )
83.55
OK
-1
0.1-00.0cm

C.. (Setup show conductivity)

Set Parameters OK

134
 Set Parameters

Setup and Calibration OK

Setup and calibration

Setup Cond OK
Setup Cond

Set Show Cond. on,

off OK
Set Show Cond
(on)

OK
Set Show Cond
(on) on
off

..1 (Setup UV averaging filter constant)

1.3

Set Parameters OK
Set Parameters

Setup and Calibration OK

Setup and calibration

Setup UV OK
Setup UV

Set Averaging OK
Set Averaging
(1.3s)

OK 0.020.040.080.160.320.641.3
2.65 10s
Set Averaging
(1.3s)
0.64

135
 () ()

() ()

C. 2 . 14 (Setup lamp run time)

Lamp Run Time ()
Set Parameters OK
Set Parameters
Setup and Calibration OK
Setup and calibration
Setup UV OK
Setup UV
Set Lamp Run Time OK
Set Lamp Run Time
Hg(2000 h)
Lamp Run Time() OK
Set Lamp Run Time
Hg(2000 h)
000

C. 2. 15 (Setup show UV)

Set Parameters OK
Set Parameters
Setup and Calibration OK
Setup and calibration
Setup UV OK
Setup UV
Set Show UV on
off OK
Set Show UV
(on)
OK

136
 Set Show UV
(on) on
off

C. 2. 16 (Setup adjust temperature)

1
15
2
3 Set Parameters OK
Set Parameters
4 Setup and Calibration OK
Setup and calibration
5 Setup Temp OK
Setup Temp
6 Set Adjust Temp.. OK
Set Adjust Temp
(25.0C)
7 OK
Warning!Temp calibr will be changed
8 OK
Set Adjust Temp
(25.0C)
25.0

C. 2. 17 (Setup show temperature)

Set Parameters OK
Set Parameters
Setup and Calibration OK
Setup and calibration
Setup Temp OK
Setup Temp
Set Show Temp on
off
OK
Set Show Temp
(on)
OK

Set Show UV
(on) on
off

137
C. 2. 18 (Setup mixer chamber volume)
2.0ml
Set Parameters OK
Set Parameters
Setup and Calibration OK
Setup and calibration
Set Mix chamber Vol. OK
Set Mix Chamber Vol
(2ml)

4 OK 0.62.05.0 12.0ml
Set Mix Chamber Vol
(2ml)
2.0

C. 2. 19 (Setup Delay UV to Frac)

(l )

UV 0.75mm 44.2
l / 10 cm
Set Parameters OK
Set Parameters

Setup and Calibration OK

Setup and calibration

Set Delay UV to Frac. OK

Set Delay UV to Frac
(380l)

4 OK
Set Delay UV to Frac
(380l)
380

C. 2. 20 l ( Calibrating the flow rate)

Set Parameters OK
Set Parameters

Setup and Calibration Start Pump Calibration OK

Start Pump Calibr.
800 pulses

3 Set Flow Rate OK

138
 Set Flow Rate
(10ml/min)
2.0

5 Enter Collected Volume OK

Enter Collected
Volume(ml)
2.05
6 Pump Calibrated Ok OK

C.2.21 (Calibrating the pressure offset)

2 Setup and calibration Change Press Offset, OK

Change Press Offset
(1005 mV)

3 Set zero pressure to calib. OK

Set zero pressure to calib
Press OK

4 Calibration Offset Donr OK

C. 3 (Menu overview)

139
140
 2 ()[Menu overview(cont.)2]

141
 3 ()[Menu overview(cont.)3]

142
 4()[Menu overview(cont.)4]

143
144
10

D. (Technical specifications)

D.1 (Operating date)

(System pump)
UV (UV measurement)
UV (UV flow cell,2mm)
UV (UV flow cell,5mm)
(Conductivity measurement)
(Conductivity flow cell)
(Fraction collection)
pH (pH measurement)
pH (pH cell)

D.2 (Physical data)

D.3 KTAprime ( KTAprime componet materials)

E . (Chemical resistance guide and chemical compatibility)

F. (Accessories and consumables)

(Optical unit)
pH (pH electrode)
(Mixer)
(Fraction collector)
10ml, 50ml (Superloop 10ml, 50ml)
150ml (Superloop 150ml)
(Cables)
(Connectors and unions)
(Tubing)
(Miscellaneous)
(User Documentation)

G. (Index)

( )

145