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Infrared Spectrometry
the study of interaction of infrared light with matter, which can be used to identify unknown
materials, examine the quality of a sample or determine the amount of components in a
mixture.
Mid-IR
- absorption, reflection, and emission spectra are employed for both qualitative and
quantitative analysis.
Near-IR
- also finds considerable use for the routine quantitative determination of certain species,
such as water, carbon dioxide, sulfur and many other simple compounds of interest in
agriculture and industry.
Far-IR
- (15 to 1000 m)
- primary use is for the determination of the structures of inorganic and metal-organic
species based on absorption measurements.
major tool for determining the structure of organic and biochemical species.
Sample Handling
- frequently the most difficult and time- consuming part of an IR spectrometric analysis.
- samples of different phases must be treated differently.
- the only common point to the sampling of different phases is that the material
containing t he sample must be transparent to IR radiation.
Gases
- the spectrum of a low boiling point liquid or gas can be obtained by permitting the
sample to expand into an evacuated cylindrical cell equipped with suitable windows.
Solutions
Solvents
shows absorptions though it is less symmetric molecule than carbon tetrachloride and
carbon disulphide.
Cells
- because of the tendency for solvents to absorb IR radiation, IR liquid cells are
ordinarily much narrower (0.01 to 1 mm) than those employed in the ultraviolet and visible
regions.
- liquid cells are frequently designed for easy disassembly and use Teflon spacers to allow
variation of path length .
Liquids
- when the amount of liquid sample is small or when a suitable solvent is unavailable, it is
common practice to obtain spectra on the pure (neat) liquid.
For Solids
- the solid sample must be ground until its particle size is less than the wavelength of the
radiation to avoid the effects of scattered radiation.
Pelleting
- in this technique a small amount of finely ground solid sample is intimately mixed with
about 100 times its weight of powdered KBr, in a vibrating ball mill.
- one of the most popular techniques for handling solid samples has been KBr.
- with many compounds, KBr pelleting produces excellent spectra that appear in many
spectral libraries.
Mull
- in this technique a small quantity of sample is thoroughly ground in a clean mortar until
the powder is very fine.
- mulls are formed by grinding 2 to 5 mg of the finely powdered sample in the presence of
one or two drops of a heavy hydrocarbon oil (Nujol).
- the IR behavior of solids can also be obtained by reflectance techniques and by the
photoacoustic method.
Qualitative Analysis
Group Frequencies
- are seldom totally invariant because of interactions with other vibrations associated
with one or both of the atoms composing the group.
- interaction effects are ordinarily small; as a result, a range of frequencies can be
assigned within which it is highly probable that the absorption maximum for a given
functional group will be found.
Qualitative Analysis
- most single bonds give rise to absorption bands at these frequencies; because their
energies are about the same, strong interaction occurs between neighboring bands.
Qualitative Analysis
Qualitative Analysis
Collections of Spectra
Qualitative Analysis
- computer matches profiles and prints a list of compounds having spectra similar to that of
the analyte.
Quantitative Applications
Quantitative data obtained with older dispersive IR instruments were generally significantly
inferior in quality to data obtained with UV visible spectrophotometers.
Quantitative Applications
- the richness of spectral features enhances the probability of overlapping absorption bands.
- the narrow path length cells required for many analyses are inconvenient to use and may
lead to significant analytical uncertainties.
- the analytical errors associated with a quantitative IR analysis often cannot be reduced to
the level associated with ultraviolet and visible methods, even with considerable care and effort.
IR reflection spectrometry has found a number of applications, particularly for dealing with
solid samples that are difficult to handle, such as polymer films and fibers, foods, rubbers,
agriculture products and many others.
Reflectance spectra can be used for both qualitative and quantitative analysis.
Types of Reflection
Specular Reflection
Diffuse Reflection
Internal Reflection
- interaction of the electromagnetic radiation on the interface between the sample and a
medium with ahigher refraction index is studied.
External Reflection
Diffuse-Reflectance Spectrometry
A complex process that occurs when a beam of radiation strikes the surface of a finely
divided powder.
ATR Spectrometry
Internal reflection spectroscopy is a technique for obtaining IR spectra of samples that are
difficult to deal with, such as solids of limited solubility, films, threads, pastes, adhesives and
powders.
ATR Spectra
Similar but not identical to ordinary absorption spectra. In general, although the same bands
are observed, their relative intensities differ.
One of major advantages of ATR spectroscopy is that the absorption spectra are readily
obtainable on a wide variety of sample types with a minimum of preparation.