World Journal of Microbiology

Vol. 3(2), pp. 115-119, September, 2017. www.premierpublishers.org. ISSN: XXXX-XXXX

WJM

Research Article

Mycological Quality Assessment of Groundnut Products

Commonly Consumed in Sokoto State, Nigeria
*1Salau IA, 2Shehu K, 3Kasarawa AB
*1
Department of Biology, Shehu Shagari College of Education, Sokoto Nigeria
2Department of Biology, Federal University, Birnin-Kebbi, Kebbi State Nigeria
3Department of Science Laboratory Technology, Umaru Ali Shinkafi Polytechnic, Sokoto Nigeria

Groundnut (Arachis hypogaea L.) is an economically important crop grown in many parts of the
world including Nigeria. However, there is paucity information with regards to the mycological
quality of groundnut products across the state. The fungal population identified to be associated
with groundnut products in three Agricultural zones of Sokoto state were isolated and
characterized using standard mycological techniques. Total fungal count (TFC) ranged from 1.2
103 to 4.7 104 CFU/g with groundnut cake from Sokoto being the most contaminated (4.7104).
Aspergillus flavus was found to be the most frequently isolated fungal species with 28.1%
followed by A. niger (20.8%) and A. fumigatus (15.4%). The lessfrequentlyencountered were
species of Penicillium notatum (1.4%). The findings from this study suggest that groundnut
products obtained from two Agricultural zones are substantially contaminated by fungi
responsible for producing Aflatoxin and Ochratoxin which could be unsafe as food or feed
ingredients. It was recommended to store groundnut with very low moisture content, evolve other
means of processing and use of varieties resistant to fungi and encourage food quality control
agencies to take up their responsibilities.

KEY WORDS: Groundnut, Fungi, Quality Assessment, Contamination and Sokoto.

INTRODUCTION

Groundnut production and trade is faced with numerous
challenges and one of the major constraint reported by
Mutegi et al. (2009), Soler et al. (2010) and Chang et al.
(2013) was fungal invasion especially by mycotoxin
producing fungi leading to loss of quality and market value
of the agricultural products. In Nigeria, Groundnut is
traditionally consumed in its boiled, roasted or other
processed form to improve its aroma, flavour and texture.
Fungi are ubiquitous and can infect groundnut at all levels
from production to processing and the supply chains
(Murphy et al., 2006). A significant level of groundnut-
mould infection occurs in the field being encouraged by
damp conditions at the time of harvest, insect infestations,
delayed harvesting as well as improper post-harvest
handling, transporting, drying, storage system and
processing methods (Reddy et al.,2013; Tiffany, 2013).
Mycological Quality Assessment of Groundnut Products Commonly Consumed in Sokoto State, Nigeria
Mold growth can results in reduced crop yields and
counterproductive reduced livestock productivity.
Mycotoxins are toxins produced by fungi belonging to the
genus Aspergillus. They are typically highly resistant and
they can be a hurdle to international trade, with increased
regulation of foods and feeds and market removal of
commodities not meeting regulatory limits.
*Corresponding author: Ibrahim A. Salau, Department of
Biology, Shehu Shagari College of Education, Sokoto,
Nigeria. Email: ibrahimasalau@gmail.com Tel:
+234(0)8053569551
Salau et al. 116
The effects of their toxic secondary metabolites, when
contaminating foods in sufficiently high levels, range from
acute to chronic, mutagenic, and teratogenic. Symptoms
of mycotoxicoses range from skin irritation to
immunosuppression, birth defects, neurotoxicity, and
death (ICMSF, 1996).
The death of some children who consumed mouldy
Groundnut cake in Ibadan was linked to fungi (Ikeorah and
Okoye, 2005). Such fungi are also gaining credibility as
sources of health hazards in houses. Furthermore, in the
current state of world concerns with bio safety and
bioterrorism, the use of mycology as weapons cannot be
ignored possibly in relation to food and/or water supplies.
Indeed, the unambiguous identification of fungi remains
the most critical area of mycological taxonomic research
because of the importance of mycology and the somewhat
confused state of the systematics (Salau et al., 2017a).
In essence, this research aims at evaluating groundnut
products samples obtained from various markets across
the three Agricultural zones in Sokoto state for mycological
contamination. This will help to determine the fungal load
of fungicontaminating groundnut products in three
Agricultural zones of Sokoto State Assessing the quality of
the products and possible public health risks posed by the
consumption of these products.
MATERIALS AND METHODS
Study Area
The study was conducted in Sokoto State, which is located
in the extreme North-western part of Nigeria. The State
geographically lies along longitude 11o 301 to 13o 501 East
and latitudes 4o to 61 North and covers a total land mass
of 26,648.48 square kilometres. Sokoto State shares
boundary with Kebbi State to the south, Zamfara State to
the east and the Republic of Niger to the north. The State
has an estimated population of about 4,742,459 people as
of 2015 with 95.9 persons per square kilometre, and 3%
growth rate annually based on 2006 population census
(NPC, 2007). Occupation of city inhabitants includes
farming, trading, commerce, with a reasonable proportion
of the population working in private and public sectors
(MOI, 2008). The soil is predominantly ferruginous tropical
type, texturally sandy and pH of the soil ranges between 6
and 7. Rainfall starts late in June and ends early, in
September but, sometimes extend to October. The
average annual rainfall is between 550 - 1300 mm with
peak in the month of August and a relative humidity of less
than 20%. The highest temperatures of 45C during the
hot season are experienced in the months of March and
April. Harmattan, (a dry cold and dusty) condition is
experienced between the months of November and
February (Salauetal., 2016b). Modern Sokoto city is a
major commercial centre in leather crafts and agricultural
products (MOI, 2008). There are 23 local government
Mycological Quality Assessment of Groundnut Products Commonly Consumed in Sokoto State, Nigeria
councils (LGC) in the State with Sokoto as the capital. The
state is divided into four (4) agricultural zones namely,
Sokoto, Gwadabawa, Isa and Tambuwal.
Collection of Samples
A total of one hundred and eighty-nine (189) samples of
Groundnut products were obtained from markets and
stores. The samples (63 each) which included groundnut
kernels, cake (kulikuli) and oil were collected at different
selling shades, stores and centres respectively. Twenty-
one (21) samples were collected from each Agricultural
zone, approximately 200g representative sample was
collected in small polyethylene packs in accordance with
Whitaker techniques (2003).
Fungal culture and isolation
The isolation of fungi was carried out using agar dilution
method as described by Pitt et al., 1992 and Salau et al.,
2017b. Ten (10) grams from each groundnut kernel and
cake powder sample were homogenized with 90 ml of
buffer peptone water and serial decimal dilutions (10-1 to
10-4) were performed. Suspected samples of fungi were
cultured onto the Potato dextrose agar. The medium was
poured into sterile Petri dish and 0.1 ml of each sample
suspension was spread-plated onto the PDA media. The
plates were incubated for 5 to 7 days at 25C. Identification
of fungal Genera and the determination of each species of
fungi were done using the keys of Klich (2002) for
Aspergillus spp. and Pitt and Hocking (1997) for
Penicillium sp. This was done by observing both
microscopic characteristics and morphology of the
colonies on PDA and SDA medium. The total fungal count
for each plate was expressed as colony-forming units per
gram of sample (CFU/g). Each genus or specie identified
was then expressed as percentage (%) of the total isolated
fungi.
The total colonies of fungi were enumerated and results
were reported in mean and average fungal counts as
described by Pitt and Hocking, (1997) and Dachoupakan
et al. (2009)
Data Analysis
The SPSS 21.0 (Windows version, IL, USA) was used for
data analysis. One-way ANOVA test was used for
comparison of means of TFC across Groundnut products
and overall (%) for fungal species. The means were
separated for test of significance by the Duncans Multiple
Range Test.
RESULTS
All groundnut product samples analyzed in this study had
fungal contamination at varying levels. A total of 355 fungal
 World J. Microbiol. 117

Table 1: Distribution of fungi contaminating Groundnut productsin three (3) Agricultural zones of Sokoto state, Nigeria
Percentage (%) of fungal species occurring in Groundnut products samples
Products Zone *TFC Aspergillus Aspergillus Aspergillus Fusarium Aspergillus Penicillium Rhizopus Mucur
(cfu/g) flavus niger fumigates solani parasiticum notatum stolonifer racemosus
Sokoto 2.6104 23.0 14.8 14.8 13.1 9.8 ----- 14.8 11.5
Groundnut
Isa 1.7103 19.7 19.7 13.1 4.9 13.1 ---- 3.3 9.8
Kernel
Tambuwal 2.8104 26.2 18.0 14.8 18.0 6.6 ----- 18.0 16.4
Mean 2.1104
Sokoto 4.7104 36.9 28.2 23.1 5.1 ---- ---- 5.1 2.6
Groundnut
Isa 1.5104 31.0 14.3 16.6 12.0 ---- 7.1 14.3 4.8
cakes
Tambuwal 3.8104 34.4 25.0 12.5 9.4 ---- 6.3 12.5 ----
Mean 3.2104
Sokoto 1.2103 40.0 26.7 33.3 ---- ---- ---- ---- ----
Groundnut
Isa 1.2103 27.8 33.3 ---- ---- ---- ---- ---- 38.9
oil
Tambuwal 1.3103 37.5 29.2 16.7 ---- ---- ---- ---- 16.7
Mean 1.2103
Overall (%) 28.1a 20.8b 15.4bc 9.0c 5.1c 1.4d 9.8c 10.4c
*TFC (cfu/g): Total fungal count in colony forming units per gram
Overall (%). Mean with different letters in the same row are statistically different (p < 0.05) according to Duncans test.

isolates belonging to 8 identified species (Aspergillus
niger, A. flavus, A. fumigatus, Fusarium, Penicillium,
Rhizopus Curvaleria and Mucur) were recovered from the
analyzed samples. Groundnut kernel samples from
Tambuwal zone had the highest total fungal count of
2.8104cfu/g while those from Isa zone had the least
fungal load, 1.7103 cfu/g (Table 1). The mean fungal load
of groundnut cake samples from Sokoto zone
(4.7104cfu/g) was higher than those in other Agricultural
Zones, groundnut oil fungal load was higher in Tambuwal
zone (1.3103cfu/g) than others with 1.3103cfu/g both of
them, although there was no significant (P>0.05)
difference in the fungal loads across all Agricultural Zones.
Aspergillus species were recovered from samples in all
locations and account for 64.3% while Fusarium species
were isolated from samples except groundnut oil samples
and at all locations. Penicillium species were present in
only groundnut cake samples from all zones except in
Sokoto. While Rhizopus species occurred only in
groundnut kernel and cakes samples from all zones.
Curvaleria was present in groundnut kernel samples, on
the overall, the incidence of Aspergillus flavus was high
(28.17%) being significantly (P<0.05) much higher than the
proportion of all other fungal isolates. Penicillium species
were the least encountered (1.4%) although its incidence
was significantly (P<0.05) different than the incidence of
Rhizopus and curvaleria lunata. The results are presented
in Table 1.
DISCUSSION
were not recovered or could not be identify in this study.
Gachomo et al. (2004) Jimoh and Kolapo (2008) reported
these fungi together with Fusarium to be the major
contaminating fungi of groundnut in storage. Therefore,
their occurrence in this food product might have originated
from the raw groundnut used in the individual groundnut
cake processing as well as the postproduction exposure of
this marketed snacks to fungal spore resident in the air.
The earlier may be a minor contributor as compared to the
latter (exposure of snacks in markets to air-borne fungal
spores) since the fungal count was higher in markets
located in the Tambuwal Agricultural Zone than Sokoto
and Isa zones locations in the state.
The presence of Aspergillus species such as A. flavus and
A. niger, Fusarium species, Penicillium species and
Rhizopus in the Groundnut cake samples is inconsonance
to the findings of many researchers including Akano and
Atanda, 1990; Jimoh and Kolapo, 2008; Makun et al.,
2010; Salau et al., 2017a), and further concluded that such
isolates may pose a toxicological threat to the consumers
since majority of the isolates of these fungal species are
toxigenic. Rhizopus is known to liberate a metabolite
rhizonin A (Wilson et al., 1984) while aflatoxins,
ochratoxins, fumonisins, trichothecenes, citrinin and
patulin are well produced during metabolism by the above
mentioned fungi. Akano and Atanda(1990) reported the
presence of fungi and aflatoxins in Groundnut cake from
Ibadan, Oyo state, Nigeria after the incidence of deaths
resulting from consumption of aflatoxin-contaminated
foods in Nigeria.

The population and type of fungi recovered from the
groundnut product samples in this study was an insight to
serious public health hazard since some of the species are
notable toxin producers while others were saprophytes;
inciting deterioration of the food material in their bid to
adapt and survive in the microenvironment. The species of
Aspergillus, Rhizopus and Penicillium isolated from
groundnut products samples corroborate to the findings of
Adebesin et al. (2001) who reported similar fungi as
contaminants of Groundnut cake alongside others which
Stored groundnuts represent a complex ecosystem in
which seed spoilage by fungi is determined by a range of
factors which can be classified into four groups; intrinsic
nutritional factors, and extrinsic, processing and implicit
factors (Magan et al., 2004). Alone or in combination
among them, these factors affect the composition of the
fungal population, inducing changes throughout the
storage period. The recovery of propagules of fungal
species in all groundnut samples and the significantly
higher incidence of A. flavus species compared to other

Mycological Quality Assessment of Groundnut Products Commonly Consumed in Sokoto State, Nigeria
Salau et al. 118
isolates, irrespective of processing method used, supports
previous studies showed that groundnut is highly
susceptible to contamination from Aspergillus species
(Soler et al., 2010; Sultan and Magan 2010;
Nyirahakizimana et al., 2013). The recovery of three
species of Aspergillus species, from the samples in this
study, agrees well with the findings of Salau et al. (2017b).
CONCLUSION
This study recovered high incidence of mycological
contamination by species of A. niger and A. flavus (66.7%)
in three Groundnut products of three Agricultural zones in
Sokoto state, Nigeria. The findings in this research
suggested that groundnut products could be contaminated
with high levels fungi (A. niger and A.flavus), which were
suspected of Aflatoxin, ochratoxin A and other mycotoxins
producing fungal species thereby making several products
of groundnut samples unsafe for human consumption and
as animal feeds ingredients for feed formulation. The
contamination of groundnut products value chain by mold
is closely related to agricultural practices in the state such
as the use of fungicide, organic manuring and un-removal
of groundnut kernel (sorting) contaminated with mold
before harvest, poor handling, storage and processing
methods after harvest among others.
Based on these findings in this study, it was recommended
that:
There is the need to enhance awareness among farmers,
processors, sellers and consumers to develop strong and
appropriate protocols for proper handling of groundnut
products from production to storage sites and to ensure
safety ofthe products for both humans and animals
consumption.
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Accepted 11 August 2017
Citation: Salau IA, Shehu K, Kasarawa AB (2017).
Mycological Quality Assessment of Groundnut Products
Commonly Consumed in Sokoto State, Nigeria. World
Journal of Microbiology, 3(2): 115-119.
Copyright: 2017 Salau et al. This is an open-access
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