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Table 1.

Test/Medium Organism Color of Color of inoculated medium Interpretation


uinoculated and colony
medium
Mannitol Salt Staphylococcus aureus Magenta/Red Yellow/Off white colonies and S. aureus is a positive mannitol fermenter
Agar (MSA) (mostly for pathogenic) yellowish zones on orange medium given the results of yellow colonies and
yellow zones on the medium.
Transparent colonies and red-
orange medium M. luteus is a negative mannitol
Micrococcus luteus Magenta/Red fermenter given the results of transparent
colonies and its retention of the mediums
redness.

MacConkey Klebsiella oxytoca Magenta Pink and white colonies, red violet K. Oxytoca is a lactose fermenter. Also a
Agar (MCA) medium gram-negative.

Pseudomonias aeroginosa Magenta Pink growth, orange medium Pseudomonias is a lactose fermenter.
Also a gram-negative.

Lysine Iron Agar Pseudomonias aeruginosa Violet Purple slant, purple deep Alkaline

Micrococcus luteus Violet Yellow deep, violet slant, white Acid


colony

Simmon citrate Enterococcus faecalis Green Green slant/deep, no growth E. faecalis is a negative .
agar
Green slant/deep, no growth
Micrococcus luteus Green M. luteus is a negative.
Blue slant/green deep, no growth

Serratia marcescens Green S. marcescens is possibly positive with


the given results of a blue slant, a change
in color of the medium.

Triple Sugar Iron Pseudomonias aeruginosa Red White colony, red slant, red deep The organisms can only utilize peptone
Agar (TSIA) aerobically.
Serratia marcescens Red White colony, red slant, yellow Organisms are non-lactose fermenters.
deep
Micrococcus luteus
Red White colony, red slant, yellow Organisms are non-lactose fermenters.
deep
Table 2.

Medium Organism Name of test Results Interpretation

NBG Escherichia coli Methyl Red (MR) Negative Both have no acid production
because of the yellow
NBG Serratia marcescens Methyl Red (MR) Negative colorization.

NBG Escherichia coli Voges-Proskauer (VP) Postive The organisms have


metabolized pyruvate further
NBG Serratia marcescens Voges-Proskauer (VP) Positive to neutral compounds.

Table 3.

Medium Organism Property being tested Results Interpretation

Starch agar Escherichia coli Starch hydrolysis Formation of blue black The starch hasnt been
complex. Negative degraded enzymatically.

Sugar fermentation broth Escherichia coli Acid and gas production Medium color is yellow, air Acid and gas production
bubbles trapped inside positive.
Sugar fermentation broth Micrococcus luteus Acid and gas production inverted tube

Tryptone broth Serratia marcescens Indole production Pink to red rings Positive for the presence of
indole.
Tryptone broth Entercoccus faecalis Indole production No visible change Negative for the presence of
indole.

Table 4.

Medium Organism Property being tested Results Interpretation


Nutrient broth Escherichia coli Reduction of product of nitrate Negative Nitrate was absent from the
medium and was further
reduced.
Nutrient broth B. Subtilis Reduction of product of nitrate Positive Nitrate was present.

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