Creative Biolabs Announced Antibody Affinity Maturation Service

With the common effort of its scientists, Creative Biolabs successfully developed
antibody affinity maturation service for antibody affinity maturation improvement.

Antibodies are used extensively in diagnostics and as therapeutic agents. Achieving

high-affinity binding is crucial for expanding detection limits, extending dissociation
half-times, decreasing drug dosages and increasing drug efficacy. In order to conquer
the shortages of common antibodies, Creative Biolabs developed affinity maturation
of antibodies service.

Weve gained extensive experience in antibody affinity maturation over the years.
We use scFv as the antibody format and monovalent display phagemid as the system
to reduce the avidity effects during antigen-binding screening, said Dr. Monika,
scientific officer of Creative Biolabs.

As Dr. Monika introduced, untargeted mutagenesis and oligonucleotide-directed

mutagenesis are utilized to construct random or defined sub-libraries to introduce a
large number of mutants of the original antibody. Antibody binders of higher affinity
are then selected by increasing the screening stringency. By constructing a series of
sub-libraries of a scFv/Fab antibody, its protocol allows increase of the affinity of the
scFv antibodies from 10 -8 to 10-9.

Phage Display Antibody Library Screening

Subsequent library screening will fish out the antibody mutants that have high affinity.
Two library screening strategies are available, "surface-panning" strategy and
"solution-sorting" strategy. In the first "surface-panning" strategy, decreasing
concentrations of antigen is surface immobilized. In the second "solution-sorting"
strategy, we have two approaches, selection based on the equilibrium constant (Kd)
and selection based on binding kinetics.

Untargeted Mutagenesis

Creative Biolabs uses an error-prone PCR integrated DNA-shuffling approach to

mutate main CDR regions during sub-library construction. If the potential of
introducing immunogenic mutations to framework positions is not a concern, they
usually use this approach to create mutations at completely random positions across
the entire VH and VL fragments.

Oligonucleotide-directed Mutagenesis

If the structure of the antibody/antigen complex is available or modeling the structure

of the antibody/antigen is possible, certain positions can be randomized at a defined
diversity to improve the affinity. Creative Biolabs is able to create any sub-libraries to
incorporate the defined mutations utilizing trimer codon technology.

In addition to the three introduced above, Creative Biolabs has other approaches used,
such as antibody affinity measurement and peptide affinity maturation.

About Creative Biolabs

Creative Biolabs is specialized in providing custom biotechnology and

pharmaceutical services that cover the full scope of biotechnology needs of early drug
discovery and drug development, such as antibody sequencing, antibody engineering,
etc.

https://news.usaonline.us/Press-Releases/Creative-Biolabs-Announced-Antibody-Affi
nity-Maturation-Service-124178