Professional Documents
Culture Documents
Expriment 2
AIM
Playing an integral role in the structure and function of proteins, amino acids are perhaps one of the
most important biomolecules as proteins themselves participate in nearly every physiological event
within a cell. In order to understand the acid-base properties of proteins and their resulting behavior
as polyionic macromolecules, we will begin by investigating the properties of their constituent amino
acids.
Protein
6
3)You will be titrating with 0.10 M NaOH, 0
which will gradually increase the pH of your 4
solution. 2
REFERENCE
www.chem.fsu.edu
vlab.amrita.edu
Albert and E.P Serjeant The determination of
Ionization Constants, Chapman and Hall, Ltd.,
London(1971). This reference gives details of
research methods for determining pKas.
Greenstein, J.P. and M. Winitz, Chemistry of
the Amino Acids, vol I, Wiley and Sons, New
York (1961) pp482-491. The method for
determining pI is explained here.
West, E.S and W.R. Todd. Textbook of
Biochemistry, Macmillan, NY, 1955, p. 311.
Datain the table are from this reference.