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Apparatus :- i) 250 conical flask with cork ii) Burette (50 ml) iii) Pipette iv) Titrated flask v) Stand vi) Measuring
cylinder vii) Beaker vii) White paper.
REAGENT :- i) Manganus sulphate solution ii) Alkalined potassium iodied iii) N?80 sodium thiosulphate iv) Concentrated
H2SO4 v) Stretch solution
PROCEDURE :-
i) The 250 ml bottle was filled with reserve tank water
ii) The bottle was fixed with 2 ml of MnSO4 & 2 ml of alkaline potassium iodide in the place where the water was fied.
iii) Fixed 2 ml of conc. H2SO4. Before adding H2SO4 the bottle was shaken and allowed it to stand for 5 minutes to complex
salt.
iv) Free iodine will be liberated and deep golden brown solution resolves.
v) Then 50 ml of treated sample was taken for titration against N/80 Na 2S2 O3 until the pale straw colour appear.
vi) Then 2 drops starch solution was added to the sample, a pale brown colour appears.
vii) It is then titrated against N/80 Na2S2O3 until the colour was completely disappeared.
viii) Total volume of Na2S2O3 was recorded.
ix) Then the mean of the records was taken.
RESULT :-
No of Observation Sample Buratte Reading (ml) Differences Mean
volume(ml) Initial Final (ml) (ml)
1. 50 0 1.4 1.4
2. 50 1.4 2.6 1.2 1.26
3. 50 2.6 3.8 1.2
COMMENT : -
Dissolved O2 plays the same role in both aquatic & terrestrial system in fact it is it s important for respiration of the aquatic
animals and plants.
According to swingle (1947) effect of dissolved O 2 level on fish population are stated below.
Dissolved O2 ( ppm ) Effects.
<1 Lethal on exposure for more than few hours.
15 Fish will survive, but reproduction and growth be poor.
>5 Normal growth and reproduction.
5 15 Good for drinking purpose.
Therefore, from this result we can conclude that this water is not suitable for fish culture. Fish will survive, but the rate growth
and reproduction will be poor.