1.

DNA DNA
2.
3.
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DNA : (Restriction endonuclease)(
)DNA (Plasmid)DNA (Ligase)
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mTOR :
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:DNA:RNA
primer

Denaturation() (,95)Annealing DNA

(,55)
Extension(,75)

PCR DNA
PCR

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transfection (phospholipids)

liposome

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