The title of the journal, Improving expansion of

pluripotent human embryonic stem cells in perfused

bioreactors through oxygen control , does not clearly
indicate that the main purpose of the study was to
industrialize human embryonic stem cell production by
introducing a method that increased the yield of the
production by 12-fold.

Unfortunately, in this journals case, the author did not

provide a list of keywords to clarify the definitions of vague or
uncommon terminologies, and jargon. Thus, I had to search
for the definitions of some terminologies used in the article
that I am not familiar of.

Upon reading the abstract, Ive already understood the

scheme. It was clear, brief, precise and written in a simple,
understandable manner, just like what an abstract should be.
However, the purpose or significance, the method used, and
experimentation part was not emphasized in the abstract
which for me is the most interesting par t. But in general, the
abstract is good.

The introduction gave an insight to the main significance

and purpose of the paper regarding stem cell technology,
specifically, greatly improving the fields of regenerative
medicine, in vitro toxicology, and drug screening. It also
introduced the new methods together with improvisation s,
accompanying the scaling-up of the process into bio
reactors, to produce better quality human embryonic stem
cells.

Ideally all researches should allow themselves to be

emulated, meaning, someone should be able to repeat their
procedures, either to confirm or debunk their findings. In this
articles case, complicated and expensive materials and
equipment were used which is somehow understandable
because they are dealing with sensitive subjects that requires
very specific optimal conditions. If a researc her wants to
investigate on this matter, the only way for him or her is
probably to seek help or assistance from a laboratory that
has all the equipment and materials required.

The main aim of the study is to lay out a foundation to

industrialize human embryonic stem cell culturing. One may
question why not just upscale the existing method used in the
laboratory, but in reality, not all laboratory set ups and
methods work on an industrial scale. Its the very reason why
we have chemical engineers and chemists.

In the study, the researchers investigated the optimal

conditions for propagation of pluripotent human embryonic
stem cells in controlled stirred tank bioreactors. Different
inoculation conditions were firstly evaluated in spinner flasks.
The best conditions to promote human embryonic stem cells
attachment were achieved by combining matrigel-coated. In
general, the result were clearly stated and discussed.
Illustrations, graphs, and images of the actual cells are
provided giving the discussion a comp lete sense along with
all the numerical findings.

The conclusion was brief and concise, answering the

problem directly, they clearly stated the increase in
production (12-fold) and the factors that contributed to its
success, primarily the control of oxyg en, and secondarily the
improved equipment and media used to attach and grow the
human embryonic cells into. The last part of the conclusion
stated that the technology developed, thus, can be
translated to clinical and industrial settings, assuring the
scalable production of cell-based products in a robust,
controlled and automated manner