TORCHAntigensSampleKit(Catalog9281)

containsantigenstodevelopahighsensitivityTORCHpanelassay

MeridianLifeScience,Inc.(MLS)hasdevelopedaconvenientTORCHsamplekitthatincludescommerciallyimportant
antigenswhichareusedinapproveddiagnosticassaysaroundtheworld.Theseantigensareexclusivelymanufactured
byMLSusingproprietarymethodsforviralextractionandpurification.TheTORCHAntigenSampleKit(Catalog9281)
containsaselectionofpopularantigenswhichareusedbytheworldstopdiagnosticcompanies.Inaddition,MLS
providesvariationsoftheseantigensandantibodiessuitableforarangeofapplications.

INTRODUCTION

TORCHisanacronymforagroupofinfectionsthatcan whichisthemostcommoncauseofcongenital
causesignificantbirthdefectsandevenfetaldeath. infectiousdisease,hasamuchhigherrateof
Toxoplasmosis transmission(10%vs.1%)formotherswithaprimary
Other:syphilis,hepatitisB,Coxsackievirus,Epstein infectioncomparedtoareactivation.
Barrvirus(EBV),humanparvovirus,andvaricellazoster
Rubella FormostTORCHorganisms,theinitialscreeningtestis
Cytomegalovirus(CMV) basedondetectionofantibodiestotheorganism.
Herpessimplexvirus(HSV) Assaysarecommerciallyavailableforthedetectionof
IgGantibodies,IgMantibodies,orbothIgGandIgM
Between23%ofallcongenitalanomaliesareattributed antibodies.Inmostcases,IgGreactivityintheabsence
toperinatalinfections,ofwhichtheTORCHagentsare ofIgMreactivityisindicativeofapastinfection,while
themostcommoncauseofinfection.TheTORCH IgMreactivityintheabsenceofIgGreactivityindicates
organismstypicallyareasymptomaticorcauseonly acurrentinfection.However,fortoxoplasmosisand
mildinfectionsinthemother;however,therearemuch CMVinfections,IgGavidityhasrecentlybeenfoundto
moreseriousconsequencesforinfectiontothefetus.In beusefulforidentifyingprimaryinfections.SinceIgM
addition,TORCHinfectionsposeagreaterrisktothe antibodiescanbeproducedfollowingreactivationorre
fetusandneonateifthemotherisactivelyinfected infectioninsomeindividuals,apositiveIgMisnot
duringpregnancy.Primaryinfections(newlyacquired necessarilyindicativeofaprimary,activeinfection.In
duringpregnancy)aregenerallymoredamagingthan addition,ithasbeendemonstratedthatIgGantibody
secondaryorreactivatedinfections.Forexampleinthe producedinthefirstfewmonthsfollowinganinitial
caseofRubella,infectiontothemotherduringthefirst infectionhasaloweraviditythanIgGantibody
12weeksofgestationleadstocongenitalrubella producedseveralmonthsoryearslater;consequently,
syndromein80%offetusandpooroutcome;however, lowavidityantibodycanbeusedtospecificallyidentify
congenitalinfectionduringthesecondhalfof highriskmotherswithaprimaryinfection.Overallto
pregnancyhasminimalrisktothefetus.ForT.gondii., protectafetusfromTORCHinfection,earlydiagnosis
althoughtheriskofmaternaltransmissionislowestin throughfirsttrimesterscreeningiscritical.MostTORCH
thefirsttrimester,congenitaldiseaseisthemostsevere infectionsareasymptomaticforthemotherbutcan
duringthisphaseandoftenresultsinfetaldeath.CMV, leadtoseriousbirthdefectsordeathforthefetusifleft
untreated.

KITCOMPONENTS

TOXOPLASMAGONDII
ToxoplasmosisiscausedbytheprotozoanparasiteToxoplasmagondii.TransmissionofT.gondiitothefetuscanresultin
serioushealthproblems,includingmentalretardation,seizures,blindness,anddeath.Theriskofcongenitaldiseaseis
lowest(1025%)whenmaternalinfectionoccursduringthefirsttrimesterandhighest(6090%)whenmaternalinfection
occursduringthethirdtrimester.However,congenitaldiseaseismoreseverewheninfectionisacquiredinthefirst
trimester.TheoverallriskofcongenitalinfectionfromacuteT.gondiiinfectionduringpregnancyrangesfrom
approximately2050%.Toxoplasmosisusuallyisdiagnosedonthebasisofantibodydetectionandinacuteinfections,as
IgGandIgMantibodylevelsgenerallyrisewithin12weeksofinfection.Specifically,thedetectionofT.gondiispecificIgM
antibodieshasbeenusedasanaidindeterminingthetimeofinfection:anegativeIgMtestresultwithapositiveIgGresult

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generallyindicatesapastinfection(sixmonthsormore).Itisimportanttonotehowever,thattheinterpretationofT.
gondiispecificIgMpositiveresultsiscomplicatedbythepersistenceofIgMantibodiesupto18monthsafterinfectionand
byfalsepositivereactionsincommercialtests.

MeridianLifeSciencemanufacturesavarietyofToxoplasmaantigensindifferentpuritiesandincommercialquantities.All
ToxoantigensarepropagatedinHeLacellsanddisplaybothIgGandIgMimmunoreactivity.Allantigensareinactivatedand
testedforinfectivitypriortofinalqualityrelease.Dependingontheantigengrade,purifiedtoxoplasmaantigensvaryfrom
>6090%Tachyzoiteprotein.

ToxoplasmagondiiAntigenGradeII
Catalog:EV9519
NativeAntigen
1vial=0.2mL
Proteinconcentration:0.81.2mg/mLbyBCA
Buffer:Phosphatebufferedsaline.
0.15MNaCl,0.05%NP40,pH7.50.2
IdealforIgMassaydevelopment
PartiallypurifiedantigencontainingNP40forgood
dispersionofantigens.
Inactivatedandtestedforinfectivitypriortofinal
qualityrelease
NOTE:Sonicatefor1second,3timesat10%amplitudebeforeuse.Avoidrepeatedfreezethaws.Storeat65oC.
ToxoplasmagondiiAntigengradeIII
Catalog:EV8131
NativeAntigen
>90%viralprotein
1vial=0.2mL
Proteinconcentration:0.5mg/mLbyBCA
Buffer:Phosphatebufferedsaline.
0.15MNaCl,pH7.50.2
IdealforIgMassaydevelopment
Highlyenrichedtoxoplasmaantigen.Purifiedby
membraneenrichmentandextraction.
Inactivatedandtestedforinfectivitypriortofinal
qualityrelease

RUBELLAVIRUS
RubellaisaviralillnesscausedbyatogavirusofthegenusRubivirusandischaracterizedbyamild,maculopapularrash.
Rubellainfectioninthefirsttrimesterofpregnancyoftenresultsinspontaneousabortionorseverefetaldefectsassociated
withCongenitalRubellaSyndrome,includingheart,brain,earoreyemalformations,deafness,microcephaly,andmental
retardation.Aggressiveimmunizationofpreschoolchildrenhasgreatlyreducedtheincidenceofrubellaepidemics,buta
needstillexistsforaccuratemonitoringoftheimmunestatus,especiallyforwomenofchildbearingage.Rubellaspecific
IgMcanusuallybedetected430daysafteronsetofillness.However,falsepositiveserumrubellaIgMtestscanoccurdue
tothepresenceofrheumatoidfactors,crossreactingIgM,orinfectionwithothervirusessuchashumanparvovirus.

MeridiansRubellavirusantigenispreparedbytheconcentrationofcellculturefluidfromcellsinfectedwithRubellavirus
strainHPV77.Therubellavirusisthenpurifiedbyaproprietarymethod.Thisantigenisverysensitivewithlow
backgroundsinELISAandothersolidphaseimmunoassays.

RubellavirusAntigenGradeIV
Catalog:6076
NativeAntigen
1vial=0.2mL
Proteinconcentration:0.5mg/mLbyOD260/280nm
>90%pure,highlypurifiedantigen
IdealpreparationforIgMantibodytesting
Buffer:10mMTris,150mMNaCl,1.0mMEDTA,pH8.08.4
Inactivatedandtestedforinfectivitypriortofinalqualityrelease

NOTE:Sonicatefor1second,3timesat10%amplitudebeforeuse.
Avoidrepeatedfreezethaws.Storeat65oC.

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CYTOMEGALOVIRUS
Cytomegalovirus(CMV)isoneoftheherpesviruses.Thisgroupofvirusesincludestheherpessimplexviruses,varicella
zostervirus,andEpsteinBarrvirus.CMVistheleadingcauseofcongenitalviralinfectionandtheviruscanbetransmitted
tothefetusduringpregnancyaftermaternalprimaryinfectionoreventuallytherecurrenceofalatentvirus.Transmission
generallyresultsinseverecomplications(hepatosplenomegaly,microcephaly,hydrocephaly,lowbirthweight,prematurity,
andmentalretardation)and1ofevery5childrenbornwithcongenitalCMVinfectionwilldeveloppermanentproblems
(suchashearinglossordevelopmentaldisabilities).

MeridianLifeScience,Inc.manufacturesavarietyofCytomegalovirus(CMV)antigenswithkeyproteinmixtures,
concentrations,andpurities.CMVantigensarepropagatedinhumanfibroblastcellsandinfectedwiththesameviral
strain,AD169.Atleast30proteinsformthecompleteinfectiousparticleofCMV.KeyimmunodiagnosticantigensofCMV
infectioninclude:pp65&p52(IgMassociated),glycoproteingB,pp150(IgM&IgGassociated),andothers.

CMVIgMConcentrateAntigen
Catalog:7511
NativeAntigen
1vial=0.2mL
Proteinconcentration:0.8mg/mLbyOD260/280nm
Buffer:0.1Mglycinebuffer,pH9.39.7
Strain:AD169
PartiallyPurified
IdealpreparationforIgGandIgMantibodytesting
Processextractsantigensprimarilyfromthenucleusandendoplasmic
reticulumoftheinfectedcellstoenhancetheamountofantigensreactingwiththeearlyIgMantibody
Inactivatedandtestedforinfectivitypriortofinalqualityrelease
NOTE:Sonicatefor1second,3timesat10%amplitudebeforeuse.Avoidrepeatedfreezethaws.Storeat65oC.

HERPESSIMPLEXVIRUS
Herpessimplexvirus(HSV)types1and2aremembersoftheHerpesviridaefamily
andproduceinfectionsthatrangefrommildstomatitistodisseminatedandfatal
disease.NeonatesareparticularlyvulnerabletoHSV1andHSV2infectionsandin
mostcases,theinfectionistheresultofverticaltransmissionduringtheperipartum
period,andmorerarelyinuteroorpostpartum.Womenwithprimaryinfections
are10to20timesmorelikelytotransmitthevirustotheirinfantsthanwomen
withrecurrentinfections.Withoutpromptinitiationoftreatment,morethan70%
ofinfectedinfantswillprogresstodisseminatedorcentralnervoussystem
diseases.SerologicalassaysthatareabletodifferentiatebetweenHSV1andHSV2areusefulforHSVdiagnosisand
usuallyrequiredetectionoftheIgGclassantibodiestotypespecificHSVglycoproteinG(gG).

HSV1Antigen HSV2Antigen
Catalog:7305 Catalog:7705
Nativeantigen Nativeantigen
1vial=0.4mL 1vial=0.4mL
Partiallypurifiedwholecelllysate Partiallypurifiedwholecelllysate
Proteinconcentration:0.250.35mg/mL Proteinconcentration:0.20.4mg/mL
byOD260/280nm byOD260/280nm
Buffer:0.1Mglycinebuffer,pH9.39.7 Buffer:0.1Mglycinebuffer,pH9.39.7
Inactivatedandtestedforinfectivitypriortofinal Inactivatedandtestedforinfectivitypriortofinal
qualityrelease qualityrelease
NOTE:Sonicatefor1second,3timesat10%amplitudebeforeuse.Avoidrepeatedfreezethaws.Storeat65oC.

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HSV1gGAntigen HSV2gGAntigen
CatalogVTI520 CatalogVTI530
Recombinantantigen Recombinantantigen
ProducedinSaccharomycescerevisae producedinSaccharomycescerevisae
1vial=0.2mL 1vial=0.2mL
Proteinconcentration:1.7mg/mLbyBCA Proteinconcentration:1.03.0mg/mLbyBCA
Buffer:0.5MMalonate,6MUrea,pH5.20.2 Buffer:50mMSodiumPhosphate,160nMKCl,
DetectstheglycoproteinGNterminalregion 5mMDTT,pH7.00.1
(aminoacids1190). DetectstheglyoproteingGspecificandunique
sequences(aminoacidsLeu343Asp649),not
presentinHSV1
NOTE:Sonicatefor1second,3timesat10%amplitudebeforeuse.Avoidrepeatedfreezethaws.Storeat65oC.

OTHERANTIGENSFORTHETORCHPANELAREAVAILABLEINADDITIONTOSAMPLESINCLUDEDINTHISKIT.
PLEASEVISITUSATWWW.MERIDIANLIFESCIENCE.COMTOLEARNABOUTOTHERPRODUCTS.

SHIPPING&STORAGE
Shipindryice,Storeat65oC

References:
CentersforDiseaseControlandPrevention(2013):
ParasitesToxoplasmosis(Toxoplasmainfection).Retrievedfrom:http://www.cdc.gov/parasites/toxoplasmosis/disease.html
Cytomegalovirus(CMV)andCongenitalCMVInfection.Retrievedfrom:http://www.cdc.gov/cmv/testingdiagnosis.html
Nahmias,AJ,WallsKW,StewartJA,etal.TheToRCHcomplexperinatalinfectionsassociatedwithtoxoplasmaandrubella,cytomegaloandherpessimplexviruses.
PediatrRes.1971;5:405406

FORRESEARCHORFURTHERMANUFACTURINGUSE

MeridianLifeScience,Inc.
5171WilfongRd./Memphis,TN38134
www.meridianlifescience.com
Orders/Inquiries:info@meridianlifescience.com
Tel:901.382.8716/Fax:901.333.8223


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