SENIOR INTER BOTANY ASSINGMENT - 2

91.The term Biotechnology was given by

a)Craig Ventre b)Robert Edward c)Karl Ericay d)Temin and Baltimore

92.EFB stands for

a)European Federation of Biotechnology
b)Eurasian Federation of Biotechnology
c)East Asia Federation of Biotechnology
d)Ethiopian Federation of Biotechnology

93.The first recombinant DNA was constructed by

a)Stanley Cohen b)Herber Boyer c)Both a and b d)Temin and Baltimore

94.___ deals with microorganisms, plants or animal cells or their enzymes to produce products and
processes useful to human. The most appropriate choice to fill the blanks is
a)Microbiology b)Biotechnology c)Molecular biology d)Biochemistry

95.Manipulation of gene and genetic material by man is fast emerging branch of science which started
with the formation of recombinant DNA molecules. This branch of science is named as
a)Recombinant DNA technology b)genetic engineering c)DNA manipulation biotechnology
d)All of these

96.The controlled use of biological agents, such as microorganisms, plants or animals for beneficial
use is called
a)biochemistry b)molecular biology c)biotechnology d)microbiology

97.The experimental manipulation of DNA of different species, producing recombinant DNA is known
as
a)gel electrophoresis b)transformation c)genetic engineering d)replication technology

98.A kind of biotechnology involving manipulation of DNA is

a)DNA replication b)genetic engineering c)denaturation d)renaturation

99.The structure involved in genetic engineering is

a)Codon b)anticodon c)RNA d)plasmid

100.Plasmid is
a)an autonomously replicating circular extrachromosomal DNA
b)an autonomously replicating circular extrachromosomal RNA
c)an circular protein molecules
d)an autonomously replicating chromosomal DNA

101.Which of the following is known as molecular scissors of DNA?

a)Ligase b)polymerase c)restriction endonucleases d)transcriptase

102.Cutting of a piece of DNA from a plasmid was done with the help of __A__ enzymes, popularly
known as __B__. Here A and B can be
a)A-Tu ligases B-Molecular glu
b)A-restriction enzyme B-Molecular scissors
c)A-joining enzymes B-Molecular glu
d)A-DNA polymerases B-Synthesising enzymes

103.Recombinant DNA has integrated fragment of

a)antibiotic resistance b)disease resistant gene c)allergy resistant gene d)all of these

104.The first restriction endonucleases type II __A__ was isolated by Smith, Wolcox and Kellery from
__B__bacterium. It was formed to cut DNA molecules at a particular point by recognizing a specific
sequence of six base pairs, known as the __C__. Here A,B and C can be
a)A-Eco-RI B-E.coli C-Restriction sequence
b)A-Eco RII B-E.coli R245 C-Recognition sequence
c)A-Hind II B-Haemohilus influenzae C-Recognition sequence
d)Bam HI B-Bacillus amyloliquefaciens C-Restriction sequence

105.In the naming of restriction enzymes the first letter is derived from __A__ name and next two
letters from the __B__and fourth letter from __C__ of ___D___ where the enzymes are extracted. A to D
the statement can be
a)A-genus B-Species C-strain D-bacteria
b)A-species B-genus C-strain D-bacteria
c)A-genus B-species C-variety D-eukaryote
d)A-species B-genus C-variety D-eukaryote

106.Match the following

COLUMN I COLUMN II
A.Eco RI 1.E.coli
B.Hind III 2.Bacillus amyloliquifaecians
C.Bam HI 3.Haemophilus influenza
D.Eco RII 4.Escherichia coli RY13
a)A-1 B-2 C-3 D-4 b)A-3 B-2 C-1 D-4 c)A-4 B-3 C-2 D-1 d)A-4 B-2 C-3 D-1

107.Special sequence in the DNA recognized by restriction endonucleases is called

a)restriction nucleotide sequence b)palindromic nucleotide sequence
c)recognition nucleotide sequence d)All of these

108.The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called
a)cloning vector b)vehicle DNA c)gene carrier d)all of these

109.There is a restriction endonuclease called Eco RI. What does co part in it stands for?
a)Coelom b)Strain of bacterium c)Coli d)Colon

110.Restriction enzymes are used to cut

a)single stranded RNA b)double stranded DNA
c)Single stranded DNA d)double stranded RNA

111.Restriction endonucleases are enzymes which

a)make cuts at specific positions within the DNA molecule
b)recognize a specific nucleotide sequence for binding of DNA ligase
c)restrict the action of the enzyme DNA polymerase
d)remove nucleotides from the ends of the DNA molecule

112.In a recombinant DNA technique, the vector refers to a

a)donor DNA, it is identified and picked up through electrophoresis
b)plasmid, transfers DNA into living cell
c)collection of entire genome in form of plasmid
d)enzyme, cuts the DNA at specific sites

113.GAATTC is the recognition site for the restriction endonuclease

a)Eco RI b)Hind II c)Eco RII d)Bam HI
114.Who discovered that restriction enzymes have the capability of cutting DNA strands in a
particular fashion, which left what has became known as sticky ends on the strands?
a)Ramdeo Mishra b)Stanley Cohen c)Herbert Boyer d)James d Watson

115.Which of the following is used in recombinant DNA technique?

a)Cell wall of virus b)Gene which produces capsid of virus
c)virus d)capsid of virus

116.The linking of antibiotic resistance gene with the plasmid vector became possible with
a)DNA ligase b)RNA ligase c)DNA polymerase d)RNA polymerase

117.The construction of the first recombinant DNA was done by using the native plasmid of
a)E.coli b)Salmonella typhimureum c)Bacillus thuringiensis d)Yeast

118.Which of the following is used in genetic engineering?

a)Plastid b)Plasmid c)Mitochondria d)ER

119.Transfer of any gene into a completely different organism can be done through
a)genetic engineering b)tissue culture c)transformation d)none of these

120.Who discovered recombinant DNA technology

a)Har Gobind Khorana b)James D Watson
c)Stanley Cohen and Herbert Boyer d)Walter Sutton and Avery

121.F.Miescher isolated DNA from

a)Bacterial cells b)pus cells c)RBC d)Virus

122.Choose the True statement

a)DNA is the largest biomicromolecule
b)The length of DNA is directly proportional to the number of nucleotides
c)The number of nucleotides increases width of the DNA decreases
d)The amount of DNA in a living is equal to the haploid content of the living organism

123.The back bone of a polynucleotide chain shows

a)Glycosidic bonds b)Hydrogen bonds c)Phosphodiester bonds d)All of these

124.The number of nucleotides in a polynucleotide chains are equal to the

a)number of nitrogen bases in that chain
b)number of ester bonds in a chain
c)Number of hydrogen bonds in a chain
d)all of these

125.Central dogma of molecular biology reveals

a)formation of Proteins from
b)Formation of RNA from DNA
c)Formation of DNA from DNA
d)All of these

126.Thymine differs from uracil

a)by having one addition methyl group
b)by lacking one addition methyl group
c)by having OH group
d)by lacking OH group
127.Choose the true statement
a)Molar amount of adenine is always equal to the molar amount of thymine
b)Molar concentration of guanine is equalied by molar concentration of cytosine
c)In DNA, sugar and phosphate occur in equimolar proportions
d)All of these

128.Which one of the following is not correct in concerned with Watson and Crick double helical
model?
a)A spiral of DNa with a length of 3.4nm show 10 base pairs
b)The distance between the adjacent base pairs is 34oA.
c)Two chains in dsDNA run parallel with one another in opposite directions
d)nitrogen bases of two chains are connect with one another by hydrogen bonds

129.Which one of the following shows less number of base pairs in a spiral
a)A-DNA b)B-DNA c)C-DNA d)Z-DNA

130.Which one of the following is most stable form of DNA

a)A-DNA b)B-DNA c)C-DNA d)Z-DNA

131.Genetical recombination of bacteria involves only one bacterium

a)Binary fission b)Transformation c)Transduction d)Conjugation

132.Genetical recombination involves transfer of genome of one bacterium into another bacterium
a)Binary fission b)Transformation c)Transduction d)Conjugation

133.Prophage means
a)first phase of bacteriophage replication
b)DNA which infect the bacterium during lysogenic cycle
c)First formed bacteriophage
d)First progeny of bacteriophage

134.During the replication of bacteriophage, the following one is synthesized in the host but not
undergoing replication
a)DNA b)Capsid c)both DNA and Capsid d)Cell wall

135.A cross is made between two plants. In the next generation 50% tall and 50% dwarf. How many
progeny of next generation are in heterozygous condition
a)All b)75% c)50% d)25%

SENIOR INTER BOTANY ASSINGMENT 2_KEY SHEET

91.C 92.A 93.C 94.B 95.D 96.C 97.C 98.B 99.D 100.A
101.C 102.B 103.D 104.C 105.A 106.C 107.B 108.D 109.C 110.B
111.A 112.B 113.A 114.C 115.C 116.A 117.A 118.B 119.A 120.C
121.B 122.B 123.C 124.A 125.D 126.A 127.D 128.B 129.C 130.B
131.B 132.C 133.B 134.B 135.C