Creative Biolabs Launches Membrane Protein Antibody Discovery Service

Growing interest is focusing on the potential of membrane protein as a breaking point of

antibody-based therapy and diagnosis for various diseases. However, there are many drawbacks in
the expression of membrane protein which serves as the antigen, including misfolding, low
expression level, difficult extraction, heterogeneous production, incomplete modification and
conformational change. These drawbacks result in low efficiency of corresponding antibody
production and hinder the progress of research project.

Many researchers know that research methods and professional practices of membrane protein
antibodies are constantly changing. Only by acquiring the latest techniques, can we achieve
success in this field.

With experience in custom membrane protein antibody research, Creative Biolabs provides
world-leading production service of antibody targeting membrane protein. It has developed
MemProArt platform to meet the increasing needs from customers. The principle is to directly
express and present target antigen of native conformation on the cell membrane, proteoliposome
or lipoparticles.

Obtaining immunoreactivity from membrane protein with native conformation, this platform has
perfectly conquered the shortcomings of traditional methods and has been proven to be of high
efficiency in generating hundreds of antibodies.

MemProArt platform has developed Phage Display Library Screening and hybridoma technique
for monoclonal antibody screening and production respectively.

Phage Display Library Screening

A large library of phage-displayed human antibodies is incubated with mammalian cells

presenting target membrane protein for antigen-antibody interaction. Phages with inert and
low-affinity antibody can be washed away, while phages with high-affinity antibody are selected
and propagated. Creative Biolabs has featured technique to greatly increase the affinity during
panning procedure, and to select the most effective phage clone for amplification and subsequent
application.

Mouse/Rat Hybridoma Production

Creative Biolabs is able to stably over-express target membrane proteins on the surface of
commercially available cell lines, such as HEK293, CHOK1, NIH3T3 and Sf9. The cells are then
used to immunize mice/rats, followed by the isolation of splenic cells. Owing to their unique
technique, Creative Biolabs could generate both rat and mouse hybridoma for rat and mouse
monoclonal antibody production.

NativeTM Antibody Discovery Platform

Backed up by antigen-specific B lymphocytes cytometry technology, the Native Antibody
Discovery Platform can screen single antigen-specific plasma cells to retrieve native antibodies
with extremely superior in vivo properties. Antibody binders directly isolated from plasma cells
not only ensures native-paired VH-VL, but also can benefit from in vivo selection and affinity
maturation.

You may be interested in Creative Biolabs. Creative Biolabs is a biotech company located in New
York. Since the establishment in 2005, it has been providing custom biotechnology and
pharmaceutical services that cover the full scope of biotechnology needs of early drug discovery
and development.

Source:
http://www.bio-itworld.com/Press-Release/Creative-Biolabs-Launches-Membrane-Protein-Antibo
dy-Discovery-Service
https://medium.com/@candyjkswift/the-latest-technique-used-in-producing-membrane-protein-ant
ibodies-d25549eabf18