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Sweeteners
II. Objective:
One goal of this lab is to determine the concentration of aspartame using a
sweetener solutions.
III. Data:
Standard
Absorbance
solution Absorbance A1 Absorbance A2 Absorbance A3
average
(mg/mL)
0.50 .112 .111 .109 .111
1.00 .215 .212 .219 .215
1.50 .319 .315 .315 .316
2.00 .418 .417 .420 .418
2.50 .499 .510 .510 .506
Equal 0.4002 g
Absorbance
0.5
0.4
0.3
0.2
0.1
0
0 0.5 1 1.5 2 2.5 3
Concentration (mg/mL)
Figure 1. Graph of Absorbance vs. concentration for aspartame-biuret stock solutions;
Samantha Wargo & Kristin Coleman. 11-6-14
IV. Calculations:
Use the equation from the trend line to find the concentration of sweetener
solutions
: = 0.2069
9.76
: = 24.4
0.4002
11.8
: = 29.4
0.4007
Use experimental amount and true amount to find percent error
| |
% = 100%
4
|24.4 26.9|
( ): 100 = 9.29%
26.9
|24.4 33|
( ): 100 = 26%
33
|29.4 30.5|
( ): 100 = 3.60%
30.5
|29.4 33|
( ): 100 = 11%
33
V. Questions:
1. Artificial sweeteners are claimed to contain zero calories. List the
sweeteners are so sweet, only a small amount is needed for one packet.
Since only a small amount is needed, the amount of calories is so low that
calculation)
carbohydrates for one packet are less than 1 gram. So the energy content