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ABSTRACT

In this Lab 7: Determination of Mass Transfer Coefficient (kLa) in bioreactor, the lab is
divided into three different experiments which are effect of aeration, temperature and
agitation to the Po2 reading. The objective of the lab is to measure the volumetric mass
transfer coefficient (kLa) of a stirred tank reactor with bubble aeration. For every experiment,
we manipulated the values of the aeration rate, temperature and the agitation rate and later
observed the changes of those parameters toward kLa value. To measure the kLa, we use static
gassing- out method where with a plot of ln (C*-CL) versus time, we can obtain kLa from the
slope of the graph. The C* is the saturated dissolved oxygen concentration where we set it as
100% as we also have calibrated the reactor to 100% when the oxygen is saturated in the
reactor. The CL is the dissolved oxygen concentration (DO) in %. We first need to calibrate
the reactors with two points (0% and 100%). Next set the operating conditions such as the
aeration rate, agitation rate and the temperature. Note that for each experiment we only
manipulated one parameter while the other two need to be kept constant. The reading of the
DO is taken once the air is supplied into the reactor at interval of five minutes. Make sure to
do the general start up first before starting the experiment. General shut down also need to be
done. The results are tabulated and the value of kLa is calculated using the static gassing- out
method. Graph of kLa versus aeration, temperature and agitation are plotted. It is observed
that for both aeration and agitation, when we increase those two parameters, the kLa will
increase as well. Direct correlation cannot be obtain for the temperature since from the plot,
the graph gives off inconstant trends thus resulting to difficulty in making conclusion on the
experiment.