Professional Documents
Culture Documents
Fig. 1. The clinical signs of white patches, redness and pale hepatopancreas in shrimp showing IMNV
infection (Nagapattinam, TN).
M S N P
st
1 step PCR
1000 bp
500 bp
328 bp
100 bp
M S N P
Nested PCR
1000 bp
500 bp
139 bp
100 bp
Fig. 2. Detection of infectious myonecrosis (IMNV) by RT-PCR using OIE primers (Farm 1,
Nagapattinam, TN). [Lanes S - Shrimp samples; N - Negative control and P - Positive control. Note that
the sample is positive by I step PCR]
PCR result for the screening of IMNV
M 1 2 3 N P M 1 2 3 N P
1 kb
500 bp
328 bp
139 bp
M 1 2 3 4 N P
1000 bp
500 bp
328 bp
100 bp
Nested PCR
M 1 2 3 4 N P
1000 bp
500 bp
139 bp
100 bp
Fig. 4. Electrophoresis in 2% agarose gel of products of IMNV by RT-PCR using OIE primers. Lanes 1-
4 Shrimp samples; N - Negative control and P - Positive control. Note that the sample number 1 and 4 are
positive by nested PCR.
Fig. 5. Histological section of shrimp muscle from abdominal segments showing coagulative necrosis and
haemocyte infiltration (40 x and 100 x, H&E stain)
K. P. Jithendran
February, 2017 CIBA, Chennai