WR are ae cettta Amino Acids and Proteins Pe aan ER 2 et ge en ee, See ee ee TS ee wre ames eS Sige fz See re nat ee NB ie we pce eal a Sau art eee ce at en fen wr cae ee Senonieem cease re RU se wos acme nats oon ee AA TTS TSF Fe BE 82 ott ae Amino acids 8.2.9 START are Br (What are amino acid?) AS centers a tr aes, ages tee ot FOG WAR APS eae OF EYE FEF Ger SPOR, ce BETS as a eS OANA BTS OR FT TRS BIST Ee FAME hee SRREL Gots zea) SoReal Se re sIofes sre FSCS STEREH ofromg 7 a, B, y Ren cae FREE 1 COR ; , YBa CH:CH,COOH CH,CH.CHCOOH ee ve ‘ = f drs TARRCARICO re airs CTR ATRET AS PTS (aa peor fs) (498 yee ars) (eo q-emratte are)ove wou sie Feito Frey ~ gration ating wie oct canitonnye wengte ect OUNEE sate firs core | ei id motte ernie ARWES MIET AN. LT MTT EC ) R cn \ On Nu, oe saTeteat APB) cows, 8 aC, Mam aT tr Bla yw erento re, wOTATEN DS 1 CROTATATTCRS py ROU RAY 8.2.9.9 BOAT aPCTT AUT (Number of amino acids) § canoer AFA te 0: AC ETAT aN TMT TH 1 ATH TAS AD WT I ary, Reet TUT ate (csTaTat afirw) | four yO REM CATIA (proline) Gat wefan, Gydronyprotine) Tat ERAT APNG eaeAa | STATE Ae AH ATT ofS ee CATOTRT (essential) % are STATE AE | 8.2.9.2 SPERM (Nomenclature) & are 7 ont afroce Ber sear fore ects fSferw mTMTATETCS ASAT FA CE | CORA COR epee catia ATT RA (NH,CH,COOH) OTM RATE a FAR UTC WAT (FHF Glykys = felt) RBIS, TRA eH FreTT wa Rca CoP WE CECH (FF Leucos = AMT); BIRCAAAR “CER oME NOM TH (WE Tyros = A); HODRA yaar CH thom UTM (IF Kyscis = HUTA) Tene | eorates SPOTS HTUTATT © (FHA) Tea TH HC ROTA CNT | CAA MAAS Gly eae Corea Ala RAR GIT BL wT Rrerwera Sony oy, eTefOs LoTR CPOE BT a FH ACR a ereican oem AE Uy MET aR GOTT CoENT D-wTRTeCT aPrompeE ersten eran wa oat OTR CNT RT COTA Ala ET LSTA OA ala WM D-CHTHAA 1 TUPAC RfSTE HTN SLT IA CoMVss aoa CATER COTATECAT UTES fe aE Sa GR- UTMTAAT (alanine)-%_ TUPAC AM wT aerate | Oe (CH, CH COOH) | NE. 8.2.2 TRA MPCET SAE (Classification of ami " ; ee 7B) UTR eC WTR PBBTT ¢ 4 a7: ao 34 7a SICA FSRAOE eT afrETRR a, B, 1 TH DHE Pisce ea FURRCE 2 AT Frere raha Refers aor fey 1 coats ersten oP co AP iy. MMS mee co eo wot oP BCA in, eatin ny (ii) RBTCATOE SORTA ATE 2 COTA senfry (Pro), eee wee My (Hvyn\ *got TAT VET Ra oPicea mec aAETE Cora Pete Sea MOTTE ctr A SS ay ORT et ATTA IMCS FTES aS Fe NA ce anc se We HS qtr, TT TOTMTR OtTRCa Beene acento tee cans Ae TS, ATTU 6 TAT od aT Lartrm amie afer a cerita Sort STAI O96 TE wa at a ee an A A 0.25 ~ 1.50 FI CARY ARICA TTR MOTH I FATS BT | ATTA Re oF eC | aad AIG OFT A eer aor raat ers arcane ; (9) SeFR Crh), (a) Sia (Van, (0) BERR 1 are ALS HAG SOSA SALT | WAT 8 afaent®a (Met), (4) (Leu), (8) WROTE (lle), (¢) ATER (Lys), @) ’ RETOTTETAT (Phe) @ (te) GABAA (Trp) | SLMRTGS SH CATE TA AT (9) safer (Arg) @ (30) RFRA (His) | (i) AP PST SCTE START APE 2 COTS TARA afr fay A FIST aT ST cam CUM ACE LES TA FEA TAA AACA AT OTT SOTATRTAT PTE AH | OTE ST mean C,H,.0 © NFB TTI CM ces a ae wR eT 1 BS Ct, ATT STF vere Aree CSTR PH GR Ae ee cs Ts HE Te eT (arse BEI AACA GTR ALCAT SCAT AA | IRRCATR WA I AACA enratteat are Cefe B 1 TeT s (9) STAIR (Ala), aR (aly), (0) BRA Cyn, (8) caffid (Ser), (@) SHFMTTATER (Asn), (%) Cen (Pro), (8) FOR (Cys), (v) BHAA Gin), (®) epratatte as (Asp) ¢ O°) pie fre Glu) | aera erence ¢ ts rst Se UE SISRET © arti acer Heer FORA TT OH TAHT eae ee eh nt Er) ACR | AAT STAT TS SCR [AI-8- : cay, eae (Ala), SRA (Wad RHE! ay erp erate fre # SC ace Ren rn ADD WS TET seer aa or OT iy wera rae afr # 208 | ua aTeATA (Lys): opraigeat af eLERara Prdifoe PTT de core bl ST-8,9 2 Fafa sacra orator PFE 1 aoe Tee } ae eee Tae arate art I senate ats [MSC ame mee -— passa H- CH- COOH aT Gy aaeren NH, | (Glycine) = CH; —- CH ~ COOH So Ala] ocerarorartnchre Pre ie | (Alanine) (A) = BEN cu -cH-coon Bit Val OL SSTATRCATT RATE TONAL cH cH | (Valine) | afrs HC NH; | 3, Rea Leu | oaamrattcaitren ative HOS cH cH, - GH- Coon (Leucine) © | abr HCA NH, oo Tet ie) Sate ea CH)~ CH: ~ CH CH - COOH (soleucine) @ on orette ie CH; NH, oF = OneTTaTRT CH; ~ CH, — CH, - CH — CH - COOH (Norleucine) naa aS NH, a. BRT Tyr CSTR Bo SBT) |G O>- cH: ~ CH- coon (Tyrosine) | cacti fis < = 2 |v. Rea Phe O-STTRCAT-B eae . Cat Ser C-SRC BRE. HO -CH,- CH Coon (Serine) (s) center fing | = NH, 0. FoR Cys OTT RT-B Hs— CHT (Cysteine) © TTT otis ing as fe Se a te i os at (G-OrNTecatcenP hee . os os ©S~CH,-cH—cOom, (Cystine oF) B-oRmetay I a3. Renee Met O-SoTaTRCT- er Rerare HyC_5 a iH (Methionine) M) ond > S~ CH) ~ CH, — CH COO! (M) NUTR fing t S de. Behar Thr oR Bay : (Threonine) (1) RC Re ua CHs~ fH ~ CH ~ COOH OH NH, 38, Trp O-aTTCAT_B_ = Cement) Fwy Ueomnatichy ‘rs ne CHa CH-COOH ne H NH, a aSURG aig — cae |e a | 7 (Proline) (Py ee “CCT S OBiig vo, wa Pyp pee zenfra ARG etal (uydroxypr- “ORE fig woBie oline) a a 2 Wa ‘aire yo, cairatoe “AD Petes HRA aE af (D) (Aspertic 3 hoy. Sa rs (Glutamic (l) 0, Peieghay Wife afro 9, dir (Glutamine) s nafeta (Arginine) é! Xe. miei (Lysine) q Fafa (Histidine) j2G fafa (Omithiney ww) ‘Git (8) Ate (R) Lys (kK) hie Ww Foote CSR as crc a evs Solan vwrtcenfty I ufc faral iin at pot ct- } ooe- cn, gue cool = fl coott NU = CU CH, yu Coon” Nu OWL NHS WELCH, = CH COOL NU N chy en \ pewstentae caverns CHL ROTELPore aoa MABE FOTPTR 8.20 cenit RCE woTaTReaT PTAA Prem s yest A (rotation ang separation or analysis of amino acids from proteins) 8 cero Corea re we Prem fe em Aare TRC APS Get eH | Reape eo com aie stba wrbtem iat wer aca FAcaed AB set FMA FAT RH | eg >. cenftrra eect 1s 2. Beoy Cora oBromyR fies cars qersteat | >. cebera wIFIreed (Hydrolysis of proteins) ¢ cenfoerrace “iter OH, HE wey ABST (proteolytic) arBTserA SayErE MAAC FA AT | ) wate SATs (TTT) (Acidic hydrolysis) 8 GADTA SEIN GIANT ON-HCI Bet H,50, WRT 12 CC 96 WHT ARCH (110°C) MTT Fa A AUG CHATS THT CHS VATS ofa, Sora Fs TRATES Beng eT | wey whe eee Fog ATA HT ROT | co. 208 HoT cons TH ze CaPRrnSIN coRCM RRA AIS eat reer Tea BPE eH 8) Fra ATTA (Alkaline hydrolysis) 8 cetfoova WAN TAR ABTS 6N-NaOH Bea Ba(OH), Val THAT Fal V1 4 srefSre wT oMMCNTTA TAPTEPTTR TA Fal CCS AEA wry f B cea— RT ORC Goffe At Bert aT (ate ITEC aS CLT ws ER STG FFA ATTY) ae centber Fara BRERA, wTaTBEA @ TRA coTS aT | 1) GSR WaT SAAN (Enzymatic hydrolysis) # SVAN aaa aforrwow, Boy celta Bie, TFG 6 Ort AE ae carbonate amerto MTT center TAS WMP FY BAT 1 arartam egfer fSfere wel FARE pH a 37°C a AN oH aa a ORCET BH RH | ew RRecarerr ae caPraT RCo TOE AT | TT MTS HARTA SHAPE ACHTR | Tal : (>) 2 effirer ys Brana @ wed ade (2) BAHIA 1 HAT OIG FA SEATG | & Berg START afro Ret cae qedteat s ceifiera oer we faiey RTT Sara sorte aPicer seth Fle shen am TATRA MBE 6 cHABRCER we Ub wETETE TET THFETS YAS Sal Fae BST | STNTRCAT oTER Relat qestemIa Sy Somer ely CEI eT eae | Rica Scrat rahe wero at VA ) wae fafewm carTATCH INA (lon exchange chromatography) 8 Fixit BCS STIR oro gehen TT a oo Bor Tale 14 erafoce aa Gorge wTMR FARM eC (CaN BIOTA Fata afer CAS sea) OTC aPCwA Fe eco FAA eT | aT RAR MTATe Bre cart ae FAAS MR SIGE BT ee a WA BH TS (band) IS BT 1 wT Cw @ aTEUeNTe fee RA Beco aw aT RH CREPE ITH) 1 FR FER CHER AT AT HN qe HEARN | TATA AR GR CmeR aac ae woe FReIace amt Reet Yee at A am Aeletce ora OTA FAR CorATEB anes ATT AE STATA APTS YAH Ta VA | wear wprateeat afTe fara (analyzer) ATT ae Sacra wafer wre faftan sey (column) MSR ATH | AR RICHTER COTTRCAT APS Re FSH STAC ePITS sete ACE oc ae aA AOE afore rors offre fifa wea were coetien FotH wea aD Rcwe EA wolf eprTecat ATS Fate Figs Raceret at ers SACS TH | dag2 6 FA SR OI Ae (BIAS A ae oan ae - D8 FC eas CAR pH CIMA pH = 5.5) « fAfER ee TE PRE Rea Bi 4 wea aps aiae Teac ate Sens ca where BIR ATA i Ui afice, “2676 crea fefen, mai, safe Reon THNICS yore SNCS SpE arabe ae yan aT aS (a) Sage orn yh (1) Fags vier tea forn-B.> 2 aration fre firey, gether Boregrecabrr Hale | ASG CAG Ree qabrrare wre ecutaN | 1) CAATA GORTAICBUNAE (Paper chromatogeaphy) & td ATR ary a onal FARR sAtacNA erAT cel cel aren Picea aif erate, Ry (relative flow) We fied MCR | wie WIA eMC TS Guance fan Af We o Sow tora mace mm (moving) tla HAT FTEet aay eL wMET eestteTt atest (hoy arr af (firt@eranentie, aieonteohr Benth) orn qracna ef ona crnlieR sfc aire (ofbitavas waforw) aera exh carafe coer wae yore er UTM slap orgs ——af fiveo1a winter out em RL yung fiveycets arya roi on) cairsonye, e AAT WI fame (#) wy oom sass sus tatt wofrywe Tar qeehnt | cavtn eae eet way STROM ‘ coven Kiwi arcs sont tet fe J 4 mateqe tpeiia arte afaeDns 6 ! se sich "He sie at ey ange eemncatl 1 TT woryer ats nee C8 5 ante yar we cu gat Fe aa a ii cutee core fsa tet wa) wx TIE. fon a # onal298 tera arenas reife fewmTR Ga fen tr are ro wre eters AAR Wr ore = WADE OTE EH | sé SEW Sa ee eee eee a wae incor Cora fefem eI woraTee freA Am a cae ate HIN AB eH [fox-8.s (~)) , SURRTT aPTE @ tae Tacos fear HAGA MANOS Ry TH AH | SCTE AEM hw wee Se TE SURTECA ATE Ee 6 TATE FHI KU! FF (Colum «age connchinfes refers arate ore Bim! ATE Yaw e MATS Tal FES | 3) Nn wEfafe 4 wear atten een sereeeTa BAT TART CoM egy Ra SCH coe afacen wie oe feoTe TAT CE (peak) SHE ae we Ferons cbarge 6 TAAL cr (peak) a | NMR acs af STATI PEER eae a SIRE (5-31, ppm) Ce Sees wan Zo ¢ CH, ~ CH - Coo™ CH; ~ COO™ 1.85 (4) f 4.46 (bm) | 4.20(g) “NH “NH 7.48 (b) 7.94 (b, m) 8) WRC sTefS (isotope dilution method) ¢ facia Safes a wafers fms ee fee SOME PE EWTN es en we aM cea ea | COM BRE WAY CO TRON ag Fats FFE ae UN ito a ata He em frees Fen eee "N aRBw a Fen ie) ee 1 am Fa ere oe aR gee we “Tefee MN smtfim « wiRomNe miefrera sees woe Fran Farrar eta femne ea en a | 3) apiraite afe (Chemical methods) 3 (0 Brkt esate rece (we ferna) ars wer marta Bittner areca wa afm ee WA | a oo afer a aTTeoE HClO,-«a =re fafa wfefae <1 wae HCIO,-43 ff514 CH\COONa wan Bec TNITCA Gata sorareen fates seme cea ea ETH | R- (HCOOH + HCI0, —— B= CH-CoOH cio, NH “NHy O-erarc tre ra Frater R-FH-COOH + HNO; ——+ R_ cy ~ COOH +N.7 | NA, OH (ld ComTete (Kjeldahl a & come Hafere SUPT Pay Femrare cas areas SUR 2a arr ears HS. AI FEAT NHL So, a sean Fa Be | Berg (NHISOFy SUPT OTS a cent ae ott FRAT SICA ARS Bie BUTE Newt fame caret erate AICS ee Rea a R-CH-cooy 80nd I ——— oso, NH; O- STRAT fins (NH,):80, + 2Na 4 + 2NaOH ——+ Na,S0, + 2NHy? +2H;,0 3.2.8 SURAT APS io a (Synthesis of o.- 4) SOOT WIT 8 SFSre ) ae aPrTes Fara ref ANT SG oC Al c.-GATCAT “en nied ere = ‘aroma 50°C wremrara Bas ace c-sorateeA ICeR MOTTA at STH ITT SU Pacerae aA o.-VyTTTCAT PAS Ae AT | PX, RCH,COOH + X, —+ RCHXCOOH + HX amino acids) & R 50°C ‘CHXCOOH + 3NH; ———> Re + NHX NH |e H,0 REHCOOH NH; OTR APTS ca race Ree ARP, STN, CRA, RIOATEA, oi, ebm @ Aa-Fre ham eee A FR | CTA (i) TFTA (Glycine) 8 CHyCOOH +Ch——> CICH,COOH’ + HCI ofits Ri X o ao hth CHOCOCH, Ho CH - coon (ii) PROTA (Cystine) ¢ Nh cai (P10) 9 ll oS r _N-cHtcoogy, ONO ay tr Gi) CoHsCH;SCH, Cl _-C(COOED: ° fF CHSCH:CaHs (i) NaOH - C GCI CeSCH:SCHSCHCOOH Ray;g np, HSCH:CHCOOH NH, NH; aa xa SCH;CH(NH;)COOH * SCH,CH(NH,)COOH (4)-fPBrRA (Cys. Cys) 8, ATHIA FeCPat (Strecker synthesis) § 4 sefere TRA cit we as ards Bile AT COONRCAT aATE Feared ar RH | CoraAwRPE aI FACET NHAC @ KCN fret ecard Bas TACT eat FRET Beets A | ATH AACA -SUTATRTAY AFT CANS a A | CTA (i) MAFF Glycine) 8 : NH,CI-+KCI = NHs + HCN + KCI 0 OH i I NH H-C-H+H-CNH-C-H Cito) cN ararcrTer aT "1 H,0. H-CH-CN Ty” Age Coon Nib NH sf26 (Gly) SUTRAsw pore ce FEARS POHPTAR Gi) SUT (Alanine) & NH . NEL cuy-CH-CN cry —U- H+ HCN CHs-C-H CHO) "? a — oe aPRAT ATRNT H'/H0, CH; -CH- COOH CNY NH “open (Ala) Gity PRfRETEA (Methionine) 8 7 HCI CH, =CH-CHO + CH;SH ~ CH;SCH,CH,CHO “yp, confer fee erent ni: ‘Hy CH - COOH CHS - CH.CH:CH-C =N — CN) CHSCH,CH, fe 01 a Aes AafeATEH (Met) @. REIT ASuaKCoTT (Hoffmann degradation) 8 4 THOS WINS TA ATALG AT we a Fa SOMA afte eww Ta Fea | CTA 7 COOEt Br KOH 7 COCR W710, = - R BN cont, SRR RCH Sioned fi a 2 Wrens ara STATES ow f 1, HRAATAETA ACAI (Erlenmeyer synthesis) & SITATCABS wHTATECAT aE eryfeR BAT at ae Seqe otefS | EARS STARRETT « CHER OTE Bateors emrcaNe oTMBES Coreen Prema Far OTTER Berg wT ace NRE 1% NaOH-aa Free fafa, Nok ‘oat Freran @ meer acm cera as Berg eH 1g rafee Dikcatiiora mecHEe RAR Ac,0- AcONa Ho—O)—cuo + yg - coon SS AEN o—-{O) cn = ¢-f0 1% NaOH NH asf arereratbats . N a \ cocati No crvenfina a | Cis ‘soreenchra (HI " HoXO)—cH= gcoon SEs #0—crh- -coon —H10, NHCOC«Hs NHCOGH, HoO)—cu, - (H~ COOH + CaH,COOH NH, (4)-BRRcaPA Tyr)Mt -C-~coon —H/ Pa im ‘aan ap ee -CH - COOH Bea, Nao CM I NH, ‘arent (Alu) vy, SRR ‘pl “nt “Aydantoin synthesis method) # ate etetcn ewctcatter aptefeaee ‘ Condensation) Fat WH | ene Beers Na-Hg ¥tat frente aa facet A SITAR PTS Beoty ey | PM, BACT. 3-oTTERRS cece ChoreBICA mec Pra 8 OT MENS Ac,0 ~ AcONa Cao —SaAON 77 CH, a O=C—nu~ Onem se, 3. ROG a C=0 O=C——nuw” RL aaron were a Reicn FATE BAT BT | SGTSTR SOMTRAT erect HB MMH | fog FE SAT Few | STATA, erm, ofa, SR, RRaFRLA, ABR, eer FR agen BPH ane aE © ecm foe Sea oe aBremacea Fors Cara ae GR | 9) Retiree at GRE AAT Dipolar fon or zwitter ion) ¢ SIA at, Rea COTTA (amine) eq Fie Eh aréferfere aire crter eMfafire aficer emcPAT wat Berg a | as ER Ts Caw cae STATA TE ake eH | aR BoM HAE CNH, © — COOH) MER SEES sce Fee erat weTOR caR AATEC MTR eH en oN eoRRE w wep SOR RR ea Roig eter ae TOTEM Mae, RETA SIA ERD NE ac | ° I pi -6 Ba R-FH-C- 0 : “NH, H)N? fora ortive IPR we Laddeste tt stro EE ACT ty faconona arma a1 erent ae F708 EF wecuit end) | CRAM TRPIAS Tee, soe arr ee wets ew AI EY NT ay | 7, ee rs Goon ara ane rH ez TENS ATATe EM © Bah 1) Beetrer (smnhunere naare) § STH SOT TT a gai aang sree etic an en STE SE ET a ae AT ag Sermx mex fafarm ara 1 wenperce aon ara, Facer —— Re aa fare | OTR FOR tg celoa aes ware oma wae aeoerhh TAC O S (amphoteric) epfea | eforeacn (writs Bat) f a f ii st -C-O+H,0 R-CH-C-0 40H —* R-FH-C i f wh NH2 wr wiseat™ (OSI AAT) t oO ° ncaa —— R-CH-C-OH “NH “NH; al - ) erecrreemets a aatenptee fer soclectric point) # SIRT ETH, COR TRI BE FoR ere wen ATM CROMRLGPAAA) STATA (ATES TTA) FA SITCATCER HUH ae STOR (ER carrm) mage sorcerer ee we | TRAE a SL ASAT OITA I TENTS CF exten wars crater rere etiam Ree TT ae FRR ATR IAT ATH SITCRICE FS win ga o8 afromme ayer fore ene wm STATE fice afew we fase (a) fee el EB pis 6 rarer ror Farce Racor TR REALS RICE Re COR ROR leroy % ayrare) 22 Fare wa AT 92 AE pH-e mm 2 aaa ofirea weet mataayfoe Fey | whe on AE pre arate afareR ace aratires com Bre amc At AE OR COTITOCA CRE TH A 7B pH OM A ae crows BTROTaTTe AS AT! I! (fee-6.0 (7) (#) wie gaa a 7 ; writ fa.04 xB ¢ wb ersten ofr sen fermen 7a STE |- SOMA APTS ae conta see PGC pla eM em may eR eee awe Fee! TT SIATLET pi fea Fox Fra 8.9 Ba] Race, eget wae HAT STTATRCT bresncen pl 7 ore 55, 3 (RA) MR 10 (FA) om are aca Fnzce ra rere! STE Eg HEN BETS CE ES RE a REAP HTS BY oe THAD COTTA oPicoR TRS Fg | earns. fr: OOOO””S~S aera vo ear aa ‘srr wo fei on cafest @.4 Fee es Qohr ew carr wo rr aa APS 9.0 epPaeranfert ee sits afis OR Tea 8 a ral fai Fra dob are pb ‘fetofea aw ) wehifertatee CaPaBT 8 TRATATECS STATA APTEATR 3100 - 3000 Xe 1500 — 1480 em! NH, arrra CaheBy BA ITS Cofa Sea | ARTGT COO” HLF FAT 1600 - 1500 em"! 4 eS BTS (band) teh ara NMR ates NH, caidrraR BeacUsTETt FACFERA (down field) fice A ICR (€15 8.00 ppm, a1 eOTarRCAT =e Foret eC ae UH | 5) are aren ae HARPER ¢ mB TST a THES STATA SPITE SAAT eo (ehiraly WTA ATC! | AN AT HTT SAG STATA (el / +) A ATATAS (1 —) BEE ACH | TORR nea crFTG, C2 At o--ATER BEAT LOTT AT D SAE L PORTA RET ARTE AE | “aio ert orca fae Greta A OTA Ny FEVER GIA CRD ae ATT AR SRetcmeT eaShe tere orca Pde PrP ergios wratten aren aaa L FARE | Lar oe mere RT er Graton ca eagle pteroma atte BA L | a AAA " * Fafa rae Seca ECHL CNET AOA Fafcrat eat» ¢ coon HO-C-H ey coon -ots:S | pm eo H- ¢ -Br Latte atrs CH eae coon coon , H Fong Nj-C-H ave 2aTarenehe are I CH; CH; L-(+)-sorenr SIG, DETEATNKe aS Be aee Kee Reem cb aris ATE @ ore Berg a tke Bee aang aioe eattentcas BM L Or emenicra AeA ETE L 1 Faferat erat-2 3 coon fooMe { wo ‘MeOH/ Ht udpon (PCs + aeRcerat ‘Con CH,OH L--)-crfia OOH HDN-C-H _. |cooH atic OL | HNIC a . eT TALS Wether aq A iH .- OH | CA TIES wefan até CH; L-C)-GistiaSe ea we Tae 74 APM] (absolute configuration) fe (W)C IV) UT ret ry VW amen (Co rage ots (Ir) wR exe EH D-wTATR 4 ; TAH § GAR | - | re b WN-C-H = Lie { Hib Ni = +. ® G CHR c (1) (11), (5) (i) (IV), (R) 8.2.4 SIRT SPCER APTA Ff (Chemical Properties of amino acids) & sorrel afore Sex wer sree errant yorces tatty afer Pte ewe aca | a B2ois STR FROTE aTeTE eora wificeter Fem seas aa mT eTdfare fre © TEE ‘iam We WOR +s) aria yocea fafararrgg ¢ 2 RIG =e FaferaT s CONTRA are =e era ame fftem sca equ aad Benq wa wa H; N — CH, —COO™ + Na&QH——> HN - CH, - COO" Na+ HO ‘RP (Gly) fe fi CH, —CH - COO" + NaOH —+ bin fin -cOo" Ne + #20 | ‘NH NH; egrnfea (Ala) cnfeare o-snfiercent tetas ++ Rett Mnt* Renih wiht CH;CONH - CH:COCH; ahr Rafe >, ACACIA Saat FRAT (Sorensen's formal synthesis) ¢ G32 SERS oars SES PE wea cote oPrcwa area rae Sa Uae fires Fe we we FRU ee Te A IE amt cPres Hatt Siete afta ene warmfats Renn eeiars tit See =F om Bar ef afore I CRS TASH PAS FS Ta RS Fa R-CH-COO+ 2HCHO— R-CH -COOH *NHs N(CH-OH); tel oe SRE TE SS (aera SA) 9) SoeTaea areas PAPREP RR 5. Praca sree Fafieat 3 STs are seer whee WES aire ox aot yes ag afees ee eat e. sare «Ge a a a (ae eTRGUS tog ag QUEERS Ss SEE cases AEE R-CH- s = (COOH + HNO, ——- R- CH- COOH + Ne + HO - oa oe ae ae N: Tt rw are oe artes 7S NH, Ce Se Ss HE wes und; < CHCOOH FS EM o-NE, 28 3s on es peg Nore SNE TEE FE game . ae Se TEs a ede Sag Be we PES wet HX — Cll; - COOH + HONO —* HO - CH, - COOH + N:7 + #0 r= Gis) paves as CH, CH COOH + HONO—+ CH - CH - COOH +N: + 29 warte Hanes ( err’ wifes PCTS ASS te om eH erica PTS . fe HN -CH- COOH GO Co) mu CHS 028-0 caine ewer L Nu- CH COOH eprrten FARE CHs ween at IMT ATE STIMASRTRTSS senitt's base) # vy, rey ITS tsa (Formation oF :shy nora aria Rive Po ‘OH eiged Ph -CH 2 N - CH ~ COONe Ph CH= 0+ HAN CH COO Ns HOV i mfocite R am : Bry wee 1) BSN YF (COOH 48 -NEH) 4 ying Fafarurrye, t orcas iMate AAUNRE FEET ea 9, SICA STF (Effect of heat) 4 AITATETAT @ arta ° ae fh, 779) G-AIRTET SiA See fog area Gera ores rare Fg Fey am aI 1 Mie CIS ETS Or, Seay OT | a-arTeen afr 2 rea SIC ae TM a saregen wratetbrtee rat TET UL STRATES A TA | vagrant wife Peerage (Sm MRT eET er ° Cl; — Cx ° HN OH A + NH, AN, NH + 2H,0 HO, 2 ‘Cc — CH, o > oO ° 2 s-aefetmaor arta (2-car) pero aire x Beeman arog wre erra Soremfm (B-FeR) TAT c, BAAS afie Sey Fa | oI CH, ~ CH - COOH CH) = CH - COOH + NH3 I | mrenteare fas Mh (2.caiPratire re) pest 4 CHs oa fe COOH ———+ CH; - CH = CH - COOH + NH corte afrs whe (Rafi ars) P-sormreenfaeTretre afr 7 Santen aPe 3 4 aera Mee aPTR wr fem Tesensfse (intramolecular) fextapeiera ager Bei STATES AI ATTA (lactam) A A | GTA CH: “N CH, - CH, -CH,-C = A AO Cils~ Cs ~ CH coo A+ inc cu AO HN-H OH / amen RaSbrefie re NH — c=0 y-RevbreearenaraRon afrs tad A, apTerPTR MoT ayy “ nits i ra 4 tN Lc cH = N / — HC cH, f. c=0 L x N=— mate Non an STATA AD aH TS Hm aI wre Ber mam ofA ‘eeu HN ~ (CH2),~ COOH + HN - (CH.),~ COOH—A-+ H,N4-(CHt),~ CO-NHH, COOH 3, WRCTACIRA MF (Formation of hydantoins) $ o-SorArRat remy Fraa SCATTER re FenTeP ROR are (areas) a a a HCI ACER are BS AL ACATTEN BSM a UREATEIA crnTAT STATA aire eaferw TITS wT (8.2.8 (b) BPA] | HCI Ph -N= C= 04 HN ~CH-COOH—= Ph -NHCO-NH-FH= COOH 3 x R RT eH és o=G ee N | Ph ere eee none SATE TT i, ct COOH —* Ph~NHCS-NH=FH= COOH in ph-N=C=S+HN-§ q i raptor aCOREDTRI PIE np o=C czSs ~r ect i eptatacat APTS, er Perec afar (Resetoh een ne re a ant ae a Oe v 3e8 sry era OA AT AN SOT samen (eacor123 ae) ata atdro Dee winM-aa fAdife FAPHIR q ° © Pint & t 7 on A ¢ 2 ox Me svoprecoorcter OLS v-L 10) > oO R meen ° ° fant erect APTS mp Arerad SeekGs oat xfer wae) CR a Te ah cat FAferut | FARR ofp, regrerecat afbrers FCbT apices sfaere wea mm wret RAB Ron sTeeyE fearerarrss afaee SA o- € CO; FS FA 1 STE Fae Rfn Rice RG ATEA A fare ean 1a BaeACEAoN were Farag, NH; @ NH3-93 AL TF Frere A TOR BEA | OTS coarferar @ eremnfaretnferest G.-NH; S61 AT TET an fg eafernra Frreeeigcra mee KE 4 Seed STA ' ° ° OH : A OH " Cun + HN -CH -coot*-OI i + CO; + NH; +RCHO R : ° : ° frontier (95 GPS) aarcentia O-NHY o ° ° ' HO. oO 3 oH Ome +NH3+ — C=N-C. a a HO ° oO oO ° mfg frees (29 TH) sy tered it 8.0 CANDTROATR Peptides eaten 05 | SRF wgratacal afirced SF Pafers oary aoe eferareeacta anaes cael ene ASTRO SISTA egret OPT SH! AQ ATOR TST cogferarrerast exferure: cantern fare cg oferta UTE Fog ries wae mi 8.0.) CAABIRS THA (Peptide bond or linkage) = afb Gad ROR STAT GSS STARE Re TER | SST AF WIESATANS (interme wary NH; SFo1 SHARE SCTE FT erie a4 GSE ST fq Goa OR ERAT eRe O-STER ees STATS erate 354 YR SE Erin ATS YS ERR EAT CHMTTRE we— cent dod -CH- | COON Hal CH C NI cit -COOIH + 1.0 RX SITET Sry R cables ae (428 coesits) gente fafaeurn & rae 8 uae RET GER CHES 1 CHMPRRTE fe egraitem wfrere wre FerrTA ee rua ae CARS sibcra ony efets ammieca fret OH ms ie 6 SEE ts SRE fre a a TACT ae TATRA idue) ea ecionea tas Te Xai tee Sa CHT UM cH, aoa STATES ara ae oe Gee TER (1.47A)-08 Br oma ard area area TAT CBI (1.32A) Perey BTAet aT 7 re = Baron wen waeftre fewen felae (am sow) [foa-8.8] 1 OH CAABIRS THA Apa THA GA ATO TE wn’ SR fomea.e 2 credits wrath wate ener Face wien eat re Catfee otfext + 8.0.9 fafeq ACT CANDIES (Different types of peptides) & comprar fe BITE faayarn SITET fre wari nee fefere eure fafeq Marre ee cn abies 28 a fen wee At ree CG ATE UIT TH STRLAABTES (dipeptide) ofrs cakara canirees arate afrs Sars “retcat efired ART SRA > 1 HABITS (tripeptide), sra® crere GETLAATTES (tetrapeptide) HA | COTA aaa 3 Maret Arey ae RT TF afreeTTtABTES (oligopeptide) 7 | axa wats alee 4 (AaDES Me SAA TF meats (Safe SF 90,909 64 FR) wea canta (aria eg ey em 1 ONT BENT A, TT rote, oes TIE eTEPAAT HR seg oes Hae *F rare at ore Fire Rt ° ° ? . W 1. : Htcrt, tf - NH CH.COO pi CH: C- NH- CH -C -NH ~ CHCOO SCH, - C- NH CHS ; Pee aa SRIATES cH CHCA, es GIS Gly - Gt ane o (Gly - Ala ~ Phe) c tog - by, sH-CHCOO _ beacere FAH Pdifow eR wearers aT HFSS HEAR CRTCATATAA (homomeric) CAABRE ayy none oe Bele VAY At fier tse wrera CRLOTATATAS (heteromeric) CAT Ry mm, ee ee (homodetic) GR CHADRG TAR FY GAT watHa THA (CAH ae RUTATTCATS (heterodetic) CHAE TA | GIA WHTATAT are wacNTeA PenTOrA we CADIS fag fog OTA TS OTT | CE WHR MBIRCS OOTATRCA APE MACHT THAT FETT FEA | COIR (Gly) ¢ SHH (Ala) PEA YS RH VRAATG Gly - Ala 4% Ala - Gly OA FACS AS 1 SR, feat comiteat afte eEe eT dara HRATRU I FET TA ARPT Gly), GMAT (Ala) g RenRersurenFR (Phe) BAIA YE za HADES Gly — Ala - Phe, Ala ~ Gly ~ Phe, Ala - Phe ~ Gi, Phe ~ Ala ~ Gly, Phe ~ Gly - Ala @ Gly ~ Phe ~ Ala 18% PaCS ACH | EIA CHABRUER OTETE afy TC AA TS AAT GAPIIT Ia US TTT | dag 8.0.9 N-A1fSs @ C-aifes STA aw WAC FA (N-terminal and C-terminal amino acid residue) 8 CHABIRS AI caiiera emg CARE] BUR ara aTeATA DAE AA AE COTA ais fica eat COMRS sit wea war eeu wre eR ace RETR OOTNTRCAT te FIC CABINS wT AR | aera BE aE OTT are RTA are eowE TT TS RN | a Fea ae eT af TS UR ATCT STRICT OPIS AERA NH, eel aiae MEI BaCRtIbA COOH eRe® FP TS aes | CORRES Co SOTA SAICCS NH, TAD YE Ae OLS Net STTATRAT aS TET TT CrreiRS a ACME Prova eH ee GR 1 TARE, CHIBRCE cy ett aces COOH FRE AS Ice One Cane TARR AAS MATRA EM et Te CoeORS TE ESTA A BE | HB BRADRTOA N- wae CATS ACG CARICA RCT g ° 9° Wey i HN-CHh CNH CH-C-LNH_cH-€_ on Cae wacEe Bite ge ee ENRREEH GRE a eee ere cen Nw Came YR TO 1 8.0.8 HABE at ATNFII (Nomenclature of peptides or polypeptides) # PIE ACRE Nets cae SOTA ‘yr oe , ste witcn ek ‘yr SST FS Sea asa Sa GT Remrterarrenting. cca ay, 1c wPttebiRcen oem so atoteemeeaiertmersty ;awe ¢ HWN~@ i wN chy NH- cH i NICH. Coo ‘ CH Wye i 1 ata 1) aie wm Siren ere fis wait ANON MY wr CoeMOeW AsCM merIO, Gly Ala GA, Gly Ali Pho MG A~ p ona 8.9. etrepfions COT Rw ory (Naturally occurring peptides) $ foe ay wits (-) THOM RATER eH | can ergo Fahey Be Com amweCM cHHDeY stem ry Laon cafirmreite Define Gary mun arm fiewe mrfce aBeTeN CAR CIN Cerfiew ey Brae wR er y 7HHf4-8.0 6 afte wary MoMA coef UME, Lean? cnetbtes Rmeee ai tes org >. wont ont my wart 2, cai 2 cafbarcatinn fecncn are i | ©, ostaratetticnta(ore eacwin (ACN) oh ani ais te Grae 2. teem » ace a) fh CA | v7 castvarraivw fin vere cA 4, aufatafon A e ctr Frome %. aoifaea MSH) w BU CRT Tet Braere faTA 4, Fuentes Brera WATT ( We FTA | : iden) & (Structure of peptides or polypepti 8.0.6 cnet tenw a fier OH Menan § PNR aI uN Se *) crbrecwa erffacant 5 70 1M enefincec COATS OM WAI Coa tem 20 Yr wrt frame FAB | NEED A Nr he BRN BH | OT306 tan aR - BASS IE ° 6 i 1 aa Hitt - CH-C a aaa 110°C, 22 i Gea Ala~ Val Hii - CH - COOH +H CH; apmntaa (Ala) Mrs oT ame aa ara at Ha MATE AH TT ER SLOTS OTE aticea Fans ¢ Remy daa em we (cots TEE, 62.2 BT . §) cebitre arate afremqcr ET (Sequence) CAT + 7H Sia aE ye CnMBRere Farr apa aE AEEF (sequence) MT FS) PGE EE omy Ut ae 9a a ere are em eon os ORT aE RT obi WARS NOTE NH, ote TAPAS COTE - COOH 27 TS ee On oy ras AE cae Sifters N. 7 Cam one oom aie wa 9a 92 Tee SEER TS TE Be Se ee, Te NE aeUaTR BIUA AE Te ELE ER Sores eee oe a Es Nay SR CI ERA RO TRE SRG TER heaAe SOTETRR BOS TE Sa EM >. N-enfes FACENS (N-Terminal analysis or degradation) 2 °°SEIR NaS cee aa ag WHER San 3 wa wim ae or UM OTe Nees ROT =m | Nokes Soe ECE Pt ; ) Crm 7a (Sanger's method) t N-Efe% AER ere weER Sie Oy Bae wo WEE | | FUR A CEREETAAE (FDNB) tem wietes ae OTE FS SE 7K or wz 2 HR SERED CARS Cine DNP-MS ES zane ee EEE NO, No, on 1%, t i v ; VF + HNC. corte — ow. N-C-ceits FDNB ro ® _ ue : DNP cre H HHO ‘ \ } —_ oxt Sy [oF COOH + crete R (93 spt Gps ane DNP aration ates (ema) a oe Zee FRE DNP-LNA RS city we aeeg mie OF Mae (OTe am eT cee, ay a, “OP feearache wae cat me sn TARR EAE Dans method) 4 a res con aie gat» SN TEE (ot re AMEN RS) CR Mee PRT a! cr CC BRL car ee Nc Far wort fred eee ST gh TT TARTAR TLC RPSL oH a TH . hui) NMez (C10) 10g bcnn OO R (- HCl) ° oaprO (unit cH e-errbiee Prom gene WM hk 6M HCI 100°C SO.NH - CH - COOH + (ambtanen ae Se N-aifee were I (STAR SPER) aPrrSa STR BES (i) GSU FafS (Eaman's method) ¢ 4 safer came (a aenAtee) SeaRersROMTETTATERS (CiHly— N= C= 8, SUT Reece) as aes Ew Bes Feige Fa secs CanBPACER ES SORE AC TC TT Ree Fee ReereneaTers CS (rc-crB7eE) sibs a afew eared Nie SOTA Pe FeaiRneTeMTeTRTETETER enya fafoen GT ase crABIRS Pract eat SET oPrrea FRO AA Aa | PTH GLC eho Hee CMT RTE AATF RE 1 STR TS RT RTT ZT aA noes Rrra FARE HRA STATA APTS MAT aT He TR ST SACHS HT A CHs-N=C=S + HN CH-CO-NH- GH -CO~ BRE 7LN2OH, featerenzonercamrenns Ry R, s CATES i] seer catty -NH-C- NH CH-CO-NH- FH-CO- RRS a_a_— R R: Rw PTC-ABRE ‘CH—NH 9 » tl O=c c=S 4 HN - CHC CABS I Nee R | coibiaces ePIC CoHs (a8 SACHA FA)™= Yao tare ain waet freifowe Parry iv) GRR THAT (Knaymatio method) 8 FGF STINRCRTEM MBER MMH MIETN HMRG (y fA RG)-c8 CY eHICS TR SUTURE she} ICH (HAI N-ATS) einen ccm CMTS AH FHP apg RC Bee TARR RAD pn pe | RD ee saFRC aT ges OAT AH SHTAT SPH ON etigg CURT! aE @ wHRTeN aT FANE eH wet Bee we Ser Naim aT fare arReeH Hef BAT UTA | X C-NBS eget (CeTerminal analysis or degradation) # cotiiaw (a1 ste AMDTOE).og ard ay ces THA GAD Waa ae SUTNTRCAT afte YO Ce MIAO ere RH Tw wilt Coearfigg Peseta | CHART MUTATE BRE TN MdtmTA Csi facaNCsTA mefweucen wert & (i Arata etefH (Reductive method) ¢ cAAbrecwA wATaie etic arta afawtice etary crercenrgcerst ce FUT aPBIeR AAA AT Nae tea Fafa waren RFEWTEW (LIATH,) RATT AIATRAGTRY (LIBH,) Wat Faenftts wea eaLGTaCeTeT BACH C. aie wyrrtecar afref erate recerees FRR FRAN TOUTE CHMOD REE AD ora eR eTATRCAT TE Fafoes cA MOF RTRRATIS UCT ORIN Faefy Say RT | COA HAN CH CONH ~ CH-CONH = CH= COOH + ROW RD” Ri Ry Ry (aa TRAE), | H.N-CH-CONH-CH-CONH-CH-COOR’ HEHE, UN-CH-CONH-CH-CONH-CH-CHLOH R R: R R, Ry Ry crs area SEE ne CH CONH- CH - COOH + HN ~ CH ~CHOH R Ry Ry (comibrRowa ae Be) RRA STH (i) OFF @ FE TUS (Schlack and Kumf method) 4 TVG cHABFowr YS ATARI AS GearenRSAIS (benzoylation) AGTH GFF (protect) FA VW 1 AW ATH NH,NCS-8 Fee Bas Wa SETS FH CATT START FTE ATCMTRROSTTRA ae ABR AT 1 TCTARCONTRT YF BS Ba(OH),-A3 ALL HA GT OHTA APTCAG TS BN | AA H:N-CH-CONH-CH-CONH-CH-CooH _CoHsCOCI ! 1 1 " CoHSCONH-CH-CONH-CH-CONH-CH-COOH | R, R; Rs : Ri Re Ry (asB RRIABRS) wuunes ° NATTA BSE * GeHsCONH ~ CH ~CONH - CH-CO-N—CH-R, —N2OH ae 1 * (aufacresy R, R, Ss Ys =0 HOTR PTS rae cent CeSCONH ~ CH ~ CONH yao R, ~GH-COOH + HN —CH-Rs (crettecen weit) = fe0 H Caer corre aPICER ATTRA | Ba(OH), HN- CH COOH Rs COTA ANGRCEA C-21S BS wa ee Aenea el aa TRC are IS SCH ATS FH BH AA ATT 0 (iydrazinolysis) + cate (a1 aforebie) cm eres ePRRTTA 7A sore 4 eam C-efere CRC are eorBhe are eoeCA rw era RRETOTES FCAT sos, a 1 IT FAH TET rn) RTA ce Cae OTR SHTER NS wae OA | NH; - NH HN ~ EH CONH - FH CONH - = COOH a R Re Rs (qe BRAS) HN- a ~ CONHNH; + H:N - a ~ CONHNH: + HIN - CH= cooH R, R, Rs c-xnfire erat PE MOTE ATE TETRA canbrerea TS O- eae TATRA facts yao HA \ waft ara ata ara ep eat CAAT HIER GN COA SE TY eure AEG SA A ARTA CAATTRE, sna apres cit 107A IN si. ree om eH STE FONTS Preemra wercareart No CGE AD | RRS ORE ATTsbi tea soit a Prato PraeryR 9, SOPHTRCA ste SATPAA (Protection of the amino group) & 7 ITH CAMBS Fecae tm A ofan CAT om wR wet fice eI) eIrCR CoP ATES SARAH SH crsterstgerdie (artrmarteata), .frsbrerstererd (BOC), HOR Greer Fey °ABA (phihaloyl) wae Bard (p-Beerrerceider) Boke 1 FT (mild) Ferma «OS @ a Cores rel STATO oP IARI BA AA AAR AAT TTE CAAA TH COREA tH HAR wey Sr ena 2. Sra af GARETAd (Protection of the carboxyl group) & If FIST Bary tty oar Coles Tay areraets rae oF as FAA ICT BA A 1 SH CHET SPA BTA spars fara, 2arka, comenite, ABBR ar ARDC a OTA OR SA A | ©. STARE aPreea anf Fret saararat ¢ eT aPICER Ind Pret afteH a4 cae. aac, eréfe, serrate, emia Toh ae area Sore eras aM aoa aa ea 8, wrdiaer yrs ~fsereAt (Activation of carboxyl gruop) & SMR a4 wae Mey frosa eam spore a aie Crab Tar Cefia er afd wat aT | erdhaee aes Fig ooTTRRRaes, brs carats = Fea ea arbre 4 afiasera meee afte Fa | oT EuN P. HN - CH) - COOH CICOOGH; P. HN - CH; ~ CO- 0 - COOC3Hs - fcr = ait ee ee (he conta ¢. TAA 414 (Coupling step) § 4 HRS THE CHATS THA ISS BI ATS STTIRTAT Bae ‘SMR aPrea afer arom arena erie erdera Ae ITC AE OT (CHAE GTA) TS Sora arora Paehgehiere Fefeeara CaaS THR AE TH | P. HN ~ CH, -CO-O- COOCHs + HyN-CH— COOCH, ——+ ion arom cit t (P= cramer see) Te Soren at ye asi P. HN - CH; ~ CO- NH- fu ~ COOCH + CO; + CHOH R eB CRATES &, Saree a4 Goat ¢ ema ST CABS was othe ag We RATA OFA (protecting Sroup) SPAR TTR TREES Bee (cHAS1RS) stew at 1 8.0.4.2 CHIBRE HeCTITIA Fafey raf ¢ >. AIGA af (Sheehan's method) & SRR afoSa BTR arena aa (4 F FT at FA (AE SoreRGRUE 8) Tal ere arr Se SINTRA Picea sree Ps Ea