NASC 9

MICROBIAL CULTURE

The method propagating microbial organisms by letting them reproduce in a certain

culture media is called a microbial culture. Microbial cultures are used to identify the organisms
living on a sample and its abundance in the sample being tested. A culture medium is a nutritive
substance, such as an agar gel or liquid medium, in which cultures of different kinds of
microorganisms are grown and propagated.

The three types of culture media based on their consistency are the solid, semi-solid and
liquid medium. A solid medium contains 2% agar where colony morphology, pigmentation and
hemolysis can be appreciated. A semi-solid medium contains 0.5% agar and a liquid medium
has no agar and is often used for inoculum preparation, blood culture, and for isolating
pathogens from a mixture.

For our group’s microbial growth, we used gelatin added with milk as our culture
medium. Three samples were used for microbial growth. We wiped one end of a cotton bud on
a remote, laptop, and door knob and incorporated it into the jelly. The culture was then left to
thrive.
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