Forensic Science International: Genetics Supplement Series 2 (2009) 384–385

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Forensic Science International: Genetics Supplement Series

journal homepage: www.elsevier.com/locate/FSIGSS

Research article

Population data about the distribution of 15 autosomal STRs and 17 Y-STRs in

South of Italy (Calabria)
A. Barbaro a,*, P. Cormaci a, A. La Marca b, S. Votano a, A. Barbaro c
a
Department Forensic Genetics, SIMEF, Reggio Calabria, Italy
b
Department Haematology, SIMEF, Reggio Calabria, Italy
c
SIMEF Director, SIMEF, Reggio Calabria, Italy

A R T I C L E I N F O A B S T R A C T

Article history: In the present study we investigated the distribution of 15 autosomal STRs loci and 17 Y-STRs loci in a
Received 11 August 2009 population from Southern Italy (Calabria). Samples for the study were obtained form more than 300
Accepted 14 August 2009 unrelated healthy individuals belonging to the analysed population since at least three generations.
Different bio-statistical values of forensic interest were calculated for the loci examined in the present
Keywords: study. The test for Hardy–Weinberg equilibrium showed that the genotype distribution was
STRs correspondent with the expected.
Frequencies
ß 2009 Elsevier Ireland Ltd. All rights reserved.
Calabria

1. Introduction DNA was extracted from donors blood/saliva samples using

In the present study we investigated the distribution of 15
autosomal STRs loci (D19S433, D3S1358, D5S8118, D8S1179, vWA,
TH01, D13S317, D21S11, TPOX, FGA, D7S820, D16S539, D18S51,
CSF1PO, D2S1338) and 17 Y-STRs loci (DYS456, DYS389I, DYS390,
DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439,
DYS635, DYS392, YGATAH4, DYS437, DYS438, DYS635, DYS448) in
a population from Southern Italy (Calabria).
Samples for the study were obtained form more than 300
unrelated healthy individuals belonging to the analysed popula-
tion since at least three generations.
2. Materials and methods
In order to minimize the possibility of contamination, all
extractions were set up in a laminar flow cabinet in a dedicated
extraction laboratory.
the IstaGene Matrix System (Biorad). DNA extracted from
samples above was quantified by the QuantifilerTM Human
and DNA and QuantifilerTM Y Human Male Quantification Kit
using a 7300 Real Time System kit following the manufacturer
protocol [1,2].
Amplification was carried out in a laboratory different from the
one dedicated to the extraction, so that amplified products never
entered the extraction laboratory. STRs amplification was per-
formed according to the AmpFlSTRs IdentifilerTM and YfilerTM PCR
Amplification kit protocols using GeneAmp PCR Systems
9700,2400,2720 thermal cyclers Female and Male Positive controls
and negative controls were used during all amplification steps.
Amplified products were analysed by capillary electrophoresis by
an ABI PRISM 3130 Genetic Analyzer employing GeneMapper 3.2
software. For samples sizing, the AmpFlSTRs IdentifilerTM and
YfilerTM Ladders and the internal lane DNA standard LIZ 500 were
used [3,4].

Table 1
Statistical parameters of forensic interest for Identifiler loci.

D19S433 D3S1358 D5S820 D8S1179 vWA TH01 D21S11 TPOX FGA D13S317 D16S539 D7S820 D18S51 D2S1338 CSF1PO

PIC 0.6820 0.7600 0.7432 0.8493 0.7532 0.8094 0.8365 0.7441 0.6918 0.7764 0.7815 0.8444 0.7700 0.5987 0.7802
HET 0.7308 0.7892 0.7764 0.8639 0.7826 0.8299 0.8507 0.7798 0.7358 0.8050 0.8066 0.8602 0.8003 0.6478 0.8067
PD 0.8313 0.8960 0.8832 0.9590 0.8876 0.9337 0.9474 0.8904 0.8346 0.9146 0.9130 0.9571 0.9107 0.6771 0.9143
MEC 0.4859 0.5956 0.5707 0.7276 0.5900 0.6672 0.7120 0.5654 0.5022 0.6124 0.6262 0.7184 0.6018 0.4074 0.6219

PIC = polymorphic information content; HET = heterozygote; PD = discrimination power; MEC = mean exclusion chance.

* Corresponding author.
E-mail address: simef_dna@tiscali.it (A. Barbaro).

1875-1768/$ – see front matter ß 2009 Elsevier Ireland Ltd. All rights reserved.
doi:10.1016/j.fsigss.2009.08.066
 A. Barbaro et al. / Forensic Science International: Genetics Supplement Series 2 (2009) 384–385 385

Table 2
Statistical parameters of forensic interest for YFiler loci.

DYS456 DYS389I DYS390 DYS389II DYS458 DYS19 DYS385a/b DYS393 DYS391 DYS439 DYS635 DYS392 YGATAH4 DYS437 DYS438 DYS448

GD 0.6173 0.6408 0.7339 0.7750 0.7807 0.7392 0.9525 0.6577 0.7453 0.6439 0.7726 0.3709 0.7448 0.6616 0.7513 0.7247
PD 0.6091 0.6344 0.7266 0.7669 0.7697 0.7316 0.9430 0.6508 0.7378 0.6373 0.7622 0.3664 0.7349 0.655 0.7435 0.715

PD = discrimination power; GD = genetic diversity.

3. Results and discussion
Different bio-statistical values of forensic interest were
calculated for the loci examined in the present study as shown
in Tables 1 and 2. The test for Hardy–Weinberg equilibrium
showed that the genotype distribution was correspondent with the
expected.
The distribution of the allele frequency in the analysed
population sample was in accordance with published data about
general Italian population [5,6].
4. Conclusions
Data from Calabria obtained in this study showed that all of the
loci have high power of discrimination and exclusion; this confirms
their utility for individual identification, paternity test and
genetics purposes.
Conflict of interest statement
None.
References
[1] InstaGene Matrix Protocol, User manual, Biorad, 2002.
[2] Quantifiler DNA Quantification kit, User Manual, Applied Biosystems, 2005.
[3] AmpFlSTRs Identifiler PCR Amplification kit, User Manual, Applied Biosystems,
2001.
[4] AmpFlSTR YfilerTM PCR Amplification kit, User Manual, Applied Biosystems, 2004.
[5] S. Presciuttini, et al., Validation of a large Italian Database of 15 STR loci, FSI 156
(2006) 266–268.
[6] S. Pelotti, et al., Microgeographic variation of Y-chromosome haplotypes in Italy,
FSI: Genet. Suppl. Ser. 1 (2008) 239–241.