THE AMERICAN COLLEGE, MADURAI

PGB 4419 BIOLOGICAL TECHNIQUES

Section A (20 x 1 = 20)
Answer all questions.
1. Dark field and bright field microscopy.
2. Resolving power
3. Aperture and Shutter speed
4. Photomultiplier
5. Quartz cuvette
6. Rotor types
7. Rf value
8. Half -life of isotopes
9. Autoradiography
10. Toluene
11. pKa value
12. Buffers
13. Isoelectric point
14. Molecular sieving
15. Paper electrophoresis
16. Restriction enzymes
17. Ligases
18. Vectors
19. Protein data base
20. Bioinformatics
Section B (5 x 7 = 35)
Answer all questions, choosing either (a) or (b). Each answer not to exceed 1.5 pages
21. a) Explain the sample preparation required for transmission electron microscopy.
(Or)
b)