PGlo Lab Report

Purpose:
The purpose of this is lab is to Understand what a bacterial plasmid is and how it is
used in biotechnology, and . Understand how genes are used to make protein in a cell.

Hypothesis:
If E. Coli are to accept the pGlo then they should resist the antibiotic and glow under the
UV light.

Procedure:
All procedure is referred to the pGlo lab under BABEC.
Observation:
● I noticed that adding the materials with the pipet is really difficult because risk of
contamination.
● During the lab the room that we stayed in was really warm and smelly.
● I noticed that we were adding very small amounts of materials by using the
micropipette.

● Does not include Antibiotic ● Does not include antibiotic resistant gene
resistant Gene ● LB and AMP present
● LB only

● Included antibiotic resistant gene ● Included antibiotic resistant gene

● LB only ● LB and AMP present
 ● Included Antibiotic resistant
gene
● LB, AMP, pGlo present

Analysis:

● DNA+, LB
○ It should show growth.
○ It should not glow because
there is no pGlo in the
bacteria.
● DNA-, LB
○ It should show growth.
○ It should not glow because
there is no pGlo in the
bacteria.

● DNA+, LB, AMP ● DNA-, LB, AMP

○ It should show growth ○ It should not show growth
because we inserted a because there is no
antibiotic resistant gene into antibiotic resistant gene in
the bacteria. the bacteria.
○ It should not glow because ○ It should not glow because
there is no pGlo in the there is no pGlo in the
bacteria. bacteria.

● DNA+, LB, AMP, pGlo

○ It should show growth
because there is antibiotic
resistant gene in the
bacteria.
○ It should glow because
there is pGlo inserted in the
bacteria.
Based on the results shown on the observation section once you add the pGlo to the
bacteria it was shown to glo under the UV light and shown growth it shows that my
hypothesis was correct. Also from this lab it is shown there is a lot of chance to have
errors for example we can have a pipet tip that is that is contaminated so the results will
be different; also we can added a different amount of materials by the micropipet so this
will result in the result to be inconsistent. Also shown in this lab, this lab can be
improved in many ways for example we can improve the lab for example we can have
more precision equipments like a timer that times to stop the heatshocking process so
we can get more consistent results. From this experiment it can also lead to the
research of what kind of organism we can insert the pGlo gene into so they can glow
under the UV light.

Conclusion:
Base on this lab I found out that if you add the pGlo into a bacteria it will glow under a
UV light. This lab was designed to help the student to understand what a bacterial
plasmid is, and how is it used in biotechnology. In addition to understand how genes
are used to make protein in a cell. I found out that pGlo can make a bacteria glow
because clearly based on the experiment when I added the pGlo to the E.Coli the end
result was the bacteria to glow under the UV light.