ANALYTICAL INSTRUMENTATION TECHNIQUES

UNIT-1: ELECTRO ANALYTICAL METHODS 9

Potentiometric methods: Electrode potential - reference electrodes -indicator electrodes -
types: hydrogen, calomel, glass, Ag-AgCl, membrane electrodes - its structure, performance
and its limitations. Measurement of pH - Potentiometeric titrations - reodox, precipitation
titrations. Conductometry: principles and method. - Conductance measurement - titrations.
Coulometry: constant current coulometry - potentiostatic coulometry - instrumentation and
applications.
UNIT-2: SEPARATION TECHNIQUES 9
Chromatographic methods: theory of chromatography - classification of chromatographic
separations - application of adsorption, partition chromatography. LC,GC and HPLC -
applications.
UNIT-3: ELECTROPHORESIS 9
Overview of electrophoresis - types - basis for electrophorotic separations - various types of
detection in capillary electrophoresis - applications to biomolecules.
UNIT-4: SPECTROSCOPY I 9
Basic principles of IR spectroscopy: - Modes of vibration of molecule - selection rule for IR
absorption - Functional group identification - Application.
MMR Spectroscopy: Basics of proton nuclear magnetic absorption - Origins of magnetic
resonance - Chemical shift - Splitting - Coupling constant - application
UNIT-5: SPECTROSCOPY II 9
Mass spectroscopy - Basic principle - instrumentation -molecular ion, meta stable ion -
recognition of molecules - Fragmentation pattern - general rule, Nitrogen rule . Explanation
of simple molecule.
TEXT BOOKS
1. Skoog, Holler and Nieman, Instrumental analysis, 5th edition, Har Court College
Publishers

2. Banwell C N Fundamentals of molecular spectroscopy, 5th ed. McGraw Hill, New Delhi
http://www.scribd.com/doc/49435881/THE-FUNDAMENTAL-OF-MOLECULAR-
SPECTROSCOPY-CN-BANWELL

2. G.Arul Das, Molecular structure and Spectroscopy, Prentice - Hall, 2002

http://upload.thishack.com/ebook%20id-8655.html
http://filesgo.org/WnknfEsz.html

4. Virtual bio instrumentation by J. B. Olensen &E.Roscow, Prentice hall, Upper Saddle

River, New jerky

REFERENCE BOOKS
1. B.Sivasankar, Bio separation, Prentice - Hall, 2003
2. http://books.google.co.in/books?id=zzfEpw65uCcC&printsec=frontcover#v=onepage
&q&f=false
3.
2. Skoog, West, D.M., Holler, F.J., Fundamentals Analytical Chemistry, 7th Edition,
Harcourt College publishers
3. G.Chatwall and S.Anand, Instrumental Methods of Chemical Analysis, Prentice Hall of
India Pvt. Ltd., New Delhi
4.Willard, Merrit, Dean and Settle, Instrumental Methods of Analysis, CBS Publishers, 1986
TEXT BOOKS
1. Skoog, Holler and Nieman, Instrumental analysis, 5th edition, Har Court College
Publishers
2. Banwell C N Fundamentals of molecular spectroscopy, 5th ed. McGraw Hill, New
Delhi
3. G.Arul Das, Molecular structure and Spectroscopy, Prentice - Hall, 2002
4. Virtual bio instrumentation by J. B. Olensen &E.Roscow, Prentice hall, Upper Saddle
River, New jerky

REFERENCE BOOKS
1. B.Sivasankar, Bio separation, Prentice - Hall, 2003
2. Skoog, West, D.M., Holler, F.J., Fundamentals Analytical Chemistry, 7th Edition,
Harcourt College publishers
3. G.Chatwall and S.Anand, Instrumental Methods of Chemical Analysis, Prentice Hall
of India Pvt. Ltd., New Delhi
4. Willard, Merrit, Dean and Settle, Instrumental Methods of Analysis, CBS Publishers,
1986