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Environmental Engineering ScienceAhead of PrintOriginal Article

Improved Temperature
Compensation for In SituHumic-Like
and Tryptophan-Like Fluorescence
Acquisition in Diverse Water Types
Wasswa Joseph
and
Mladenov Natalie
Published Online:5 Apr 2018https://doi.org/10.1089/ees.2017.0315

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Abstract
The use of in situ fluorescence sensors is gaining popularity in water and wastewater
treatment and water reuse industries. Tryptophan-like (TRP-like) fluorescence is
particularly well suited to tracking fluorescent compounds derived from bacteria as well as a
wide range of chemicals of concern for drinking water. Despite the merits of fluorescence
sensors, they are affected by environmental factors such as temperature, which can
influence photophysical properties of fluorescence. Although temperature correction factors
have been well established for humic-like fluorescent dissolved organic matter (fDOM)
sensors, there is a need to assess the suitability of existing temperature compensation
models in diverse water types and derive new corrections that apply specifically to TRP-like
fluorescence. Temperature experiments were conducted using a submersible fluorometer
and a range of water types, including creek water, water from different treatment stages at
a water reuse facility, synthetic wastewater, and prepared samples containing TRP and
fulvic acids. Results from this study revealed that at low fluorescence intensities, no
 temperature corrections were needed for TRP-like and fDOM fluorescence sensors. For
most water types, a published temperature compensation constant for fDOM fluorescence
produced a fairly good fit to the reference temperature, but had limited applicability for TRP-
like fluorescence. The best fit to the reference temperature for TRP-like fluorescence was
achieved using a method to minimize the root mean square error (RMSE) between
modeled and measured TRP-like fluorescence. The application of temperature corrections
resulted in a difference of as much as 2500 RFU in water types with high DOC
concentrations at high (>30°C) and low (<10°C) temperatures, which has important
implications for the use of fluorescence sensors for source water protection and other
monitoring applications in these conditions. For TRP-like fluorescence, empirical
relationships and temperature compensation constants were also derived and
recommended for samples with varying tryptophan and organic carbon concentrations.

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Copyright 2018, Mary Ann Liebert, Inc.

To cite this article:

Wasswa Joseph and Mladenov Natalie.Environmental Engineering Science.http://doi.org/10.1089/ees.2017.0315

o Online Ahead of Print:April 5, 2018

Keywords

o C3 submersible fluorometer
o fDOM
o in situ fluorescence sensors
o temperature correction
o tryptophan like

Publication History

Received 1 August 2017

Accepted 27 February 2018
Published online 5 April 2018
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