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The medical field uses a variety of rapid diagnostic tests, including tests for urine, premature
rupturing of membranes, and occult blood in stools. These test all rely on simply chemical
reactions that produce a visible change in color. Many of these tests can be purchased by anyone
without a prescription, such as pregnancy urine tests.
This type of rapid testing is generally qualitative, which give a visible positive or negative result,
but without a specific quantity. For example, a pregnancy urine test can be positive due to the
presence of the hCG hormone, but it does not indicate anything about how much hormone is
present. A blood hCG test can give a specific concentration of hCG, or a quantitative result, but it
requires more time, and often more sophisticated instrumentation.
In some cases, qualitative tests are looking for a positive or negative result. For example, an occult
blood test will be positive if there is blood in the stool, but negative in any other case. How much
blood is not important at that point. The same argument can be made for a pregnancy test.
However, in many cases, qualitative tests require a standard to compare to. One form of a standard
is a standard curve, which was used during the CAD experiment. Another can be a pre-printed
standard, such as those used for urine tests. This standard can provide an approximate
concentration of a substrate to provide additional diagnostic information.
No matter which qualitative test is performed, it is essential to know what a positive and negative
result looks like. Imagine giving a pregnancy test and not knowing what a positive result looked
like—the data is useless! In this experiment you will be identifying an unknown salt solution. As
a comparison, you will first need to test known compounds to recognize a positive test when you
see it. Since salts are a mix of a cation and an anion, it will be essential to identify both parts
independently, looking for both positive and negative results.
Positive results can be in the form of gas evolution (bubbles), precipitates, color changes, or
temperature changes. Negative results are the lack of a visual change (which does not mean nothing
is happening, just nothing is happening visually). It is also important to pay close attention to the
speed of a reaction—some happen very quickly, and some will take a few minutes. Negative results
are just as important as positive results, as false positives happen, and additional data helps support
the correct answer.
To further avoid false positives (or false negatives), it is important to understand the predictive
nature of chemistry. When reactivity rules or trends are understood, the observations (and,
subsequently, the chemistry) makes sense. In that vein, this experiment also requires an
understanding of what is going on. Why does a solid form? Because the product is insoluble. Why
do bubbles appear? Because there is a reaction that forms a gas. Understanding the chemistry
greatly reduces false positives or false negatives, because understanding eliminates guess-work.
These patterns can be found in solubility tables, reactivity tables, and other tables that have been
printed in textbooks for generations.
For this lab, you will be given one of the following unknowns. By identifying both the cation and
anion portion (by comparison to known compounds), it is possible to identify the unknown. The
potential cations are aluminum, ammonium, barium, calcium, iron, magnesium, potassium,
sodium, or zinc, and the potential anions are bromide, carbonate, chloride, hydroxide, iodide,
oxide, phosphate, or sulfate.
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2 | Qualitative Analysis
Use pictures to illustrate the procedure required for this experiment in the space below.
4 | Qualitative Analysis
CHEMICALS
• 0.1 M aluminum nitrate, Al(NO3)3 • 0.1 M sodium phosphate, Na3PO4
• 0.1 M barium nitrate, Ba(NO3)2 • 6 M nitric acid, HNO3
• 0.1 M calcium nitrate, Ca(NO3)2 • 0.5 M ammonium molybdate,
• 0.1 M ferric nitrate, Fe(NO3)3 (NH4)2MoO4
• 0.3 M potassium nitrate, KNO3 • 0.5 M sodium sulfate, Na2SO4
• 0.1 M magnesium nitrate, Mg(NO3)2 • 0.1 M sodium iodide, NaI
• 0.1 M sodium nitrate, NaNO3 • 0.1 M sodium bromide, NaBr
• 0.1 M ammonium nitrate, NH4NO3 • 6 M acetic acid, CH3COOH
• 0.1 M zinc nitrate, Zn(NO3)2 • cyclohexane
• 6 M sodium hydroxide, NaOH • 0.5 M sodium hypochlorite, NaOCl
• 1 M sodium carbonate, Na2CO3 • 0.1 M sodium chloride, NaCl
• 6 M hydrochloric acid, HCl • 0.1 M silver nitrate, AgNO3
HAZARDS
Acids and bases can be corrosive and cause severe burns. Gloves, goggles, and proper clothing
are required. In case of contact, affected clothing should be removed immediately and skin rinsed
with abundant amounts of water until medical help arrives. Use the safety shower if necessary.
Other solutions may be toxic or corrosive. Silver nitrate will stain skin and clothes. Refer to the
applicable SDS for additional information regarding the chemicals used in this experiment.
TECHNIQUES
This experiment requires the use of a variety of techniques, which are explained below. Please
refer to these instructions before attempting this experiment.
Heating
Your TA will set up a hot water bath with a large beaker at the end of the bench near the windows
or in a fume hood. Make sure the beaker does not boil to dryness—alert your TA if the water gets
low. Use the bath carefully as needed.
Mixing
Small amounts in a test tube may be mixed by carefully tapping a finger against the side of the
tube near the bottom. Never point the open mouth of a test tube toward yourself or others.
Indicator/Litmus Paper
Use a small amount of solution on the end of a glass stirring rod and touch it to the paper. Do not
dip the paper into your test tube.
Al(NO3)3 –
Ba(NO3)2 –
Ca(NO3)2 –
Fe(NO3)3 –
KNO3 –
Mg(NO3)2 –
NaNO3 –
NH4NO3
Zn(NO3)2 –
Unknown
Qualitative Analysis | 7
Question 1. Why might phosphate or carbonate show a positive result in the hydroxide
test? Include appropriate chemical reactions in your explanation.
8 | Qualitative Analysis
Carbonate
Phosphate
Sulfate
Hydroxide
Iodide
Bromide
Chloride
Oxide
Question 2. Why would the presence of bromide or iodide show up as a false positive
in the test for chloride? Include appropriate chemical reactions in your explanation.
QUALITATIVE ANALYSIS
Name:________________________________
Section:_______________________________
Date:_________________________________
2. Using your data, solubility tables, and your understanding of chemical reactivity, write the
products and the state of matter for the products (solid, aqueous, or gas) that form when the
following compounds are mixed with aqueous NaOH.
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10 | Qualitative Analysis
3. Write balanced net ionic equations (remember states) for the each of the reactions below.
Na2CO3 + HCl
Na2SO4 + BaCl2
NaCl + AgNO3
4. Based on your experimental results, write a conclusion for this experiment. This should be in
paragraph format and include the purpose, your results, and the identification of your unknown.
Support your claims with experimental evidence (but do not restate the procedure). Be sure to
include an any errors that may have affected your experimentation.
Upload all procedure and data pages (pages 3, 6–10) as a single PDF document to Canvas within 24 hours from
the end of lab. A PDF image of each page can be obtained using an app on your phone, a scanner at the library,
or another method of your choice.