ABSTRACT

Human Mesenchymal Stem Cell (h-MSC) has three special character. Each can
be differentiate into several particular lineages, it has immunomodulating ability and
ability to produce tropical factors. This trait is very useful in the medical field.
Efficiency in h-MSC cultures is influenced by environmental condition, precision in
determining pasase time and treatment, this has a significant impact on the quality of
culture, which can be measured from CD73, CD90, and CD105 expression. This
studied about the effect of the 3rd and 5th phases of MSC on expression of CD105 to
find the best phase in harvesting h-MSC cell culture.
The experimental method was iv vitro, using post test only with control group
at two time phase h-MSC (third and fifth phase groups), three replication groups. The
measurement of CD105 expression can be read using Flowcitometry. The results of
the research data were tested using Independent sample test.
The results of the study showed the percentage of expression of CD105 at 3rd
phase (56,83% ± 0,89%), 5th phase (67% ± 0,83%). The results of the Independent
sample test showed a significant difference p = 0.001 (p <0.05).
This study shows that the phases time in the culture harvesting has an effect
on the expression of CD105 h-MSC.
Keywords: Mesenchymal Stem Cell, cell culture, the cell phase.